Preliminary identification and characterisation of leptin gene polymorphism in Indian goats

Maitra, A.; Sharma, Rekha; Pandey, Ashok; Singh, L. P.; Mandakmale, S. D.; Mishra, Bina

doi:10.1080/09712119.2013.795895

Cited by 14 publications

(8 citation statements)

References 11 publications

(9 reference statements)

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“…Several authors have reported about the candidate genes responsible for the growth and milk production traits and suggested their use as molecular markers related to milk production and meat quality in goats (Maitra et al 2013). Being the important components of somatotropic axis, insulin-like growth factor 1 (IGF-1), insulin-like growth factor binding protein-3 (IGFBP-3), and growth hormone receptor (GHR) are believed as main regulators of postnatal somatic growth, stimulating anabolic processes such as cell division, skeletal growth, and protein synthesis (Clemmons et al 1987).…”

Section: Introductionmentioning

confidence: 99%

Sequence characterization and genetic variability analysis of GHR, IGF1, and IGFBP-3 genes in nine Indian goat breeds

Sharma¹,

Dutt²,

Jayakumar³

et al. 2014

Journal of Applied Animal Research

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The genes that are part of the somatotropic axis play a crucial role in the regulation of growth and development of goat and other livestock animals. The identification of genetic polymorphisms in these genes will enable us to evaluate the biological relevance of such polymorphisms and to gain a better understanding of quantitative traits like growth. The present study was undertaken with the objective to analyze the caprine growth hormone receptor (GHR), insulin-like growth factor 1 (IGF1), and insulin-like growth factor binding protein 3 (IGFBP-3) genes to gain insight into the polymorphisms in these genes across different indigenous goat breeds in a panel of 80 animals. The comparative gene sequence analysis in all Capra hircus breeds under study revealed 31 single nucleotide polymorphisms (SNPs) across different loci of GHR, IGF1, and IGFBP-3 genes. Out of 31 SNPs detected, 18, 8, and 5 SNPs were present in IGF-1, IGFBP-3, and GHR genes, respectively. All the mutations pointed out in the GHR and IGF1 genes were found to be synonymous and 6 of 8 SNPs in IGFBP-3 were found to be nonsynonymous. All the detected mutations were reported here for the first time. The frequencies of the SNPs were assessed in nine different goat breeds. Across breeds, the average frequency of the least frequent alleles ranged from 0.02 to 0.17.

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Section: Introductionmentioning

confidence: 99%

Sequence characterization and genetic variability analysis of GHR, IGF1, and IGFBP-3 genes in nine Indian goat breeds

Sharma¹,

Dutt²,

Jayakumar³

et al. 2014

Journal of Applied Animal Research

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“…Further, presence of only a single genotype in the fragment of LEP gene under study may be an indication that the genotype present might be the favourable genotype without any mutation. However, Maitra et al, (2014) conducted a study for preliminary identification and characterisation of leptin gene polymorphism in Indian goats and discovered seven novel SNPs. Wang et al, (2015) detected six novel SNPs in 411 individuals from five Chinese goat breeds using DNA sequencing.…”

Section: Resultsmentioning

confidence: 99%

Analysis of Leptin Gene Polymorphism in Assam Hill Goat and their Association with Growth

Sarmah¹,

Laskar²,

Nahardeka³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

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“…For LEP gene polymorphism, exon 3 and intron 2 were studied in different research which had a significant association with productive and reproductive traits in ovine breeds (Liefers et al 2002 ; Abousoliman et al 2020 ; Singh et al 2009 ; Maitra et al 2014 ; Abousoliman et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Association of PRLR, IGF1, and LEP genes polymorphism with milk production and litter size in Egyptian Zaraibi goat

El-Shorbagy

Abdel-Aal

Mohamed

et al. 2022

Trop Anim Health Prod

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Studying variation in genes responsible for physiological characters is important to enhance goat productive and reproductive efficiency. This study aimed to detect specific nucleotide polymorphisms in prolactin receptor (PRLR), insulin-like growth factor (IGF1), and leptin (LEP) genes and their correlation with milk production (MP) and litter size (LS) traits in Zaraibi goat. PCR-SSCP products of different patterns of each gene were sequenced and aligned to reveal two mutations (T > C) and (G > A) in 3′UTR of PRLR gene and registered on NCBI with accession numbers OM418863 for TT and OM418864 for CT, while (G > A) variation was registered as OM418861 for GG and OM418862 for AG in exon 10. TT, CT, AG, and GG genotypes were distributed in the studied animals with frequencies 0.43, 0.57, 0.65, and 0.35, respectively. While alleles C, T, A, and G frequencies were 0.28, 0.72, 0.32, and 0.68, respectively. CT and AG genotypes associated significantly (P < 0.05) with higher MP and LS, respectively. By studying the haplotypes of PRLR, C-A and T-A were associated with the highest and the lowest level of MP, respectively. For LS, T-A and C-G showed significant correlation with the highest and the lowest rate, respectively. Regarding IGF1 gene, two polymorphisms were detected; T74C at exon 4 which registered on NCBI as OM418860, and combined mutations as ins. G470, A531G, and T534C (PP genotype) at 5′ flanking region that registered as OM418859. For LEP, only one polymorphism was found in intron 2 (G281A) which submitted to NCBI as OM418855. All detected polymorphisms have shown to be involved in regulating the MP or LS as reproductive traits in goat.

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Preliminary identification and characterisation of leptin gene polymorphism in Indian goats

Cited by 14 publications

References 11 publications

Sequence characterization and genetic variability analysis of GHR, IGF1, and IGFBP-3 genes in nine Indian goat breeds

Sequence characterization and genetic variability analysis of GHR, IGF1, and IGFBP-3 genes in nine Indian goat breeds

Analysis of Leptin Gene Polymorphism in Assam Hill Goat and their Association with Growth

Association of PRLR, IGF1, and LEP genes polymorphism with milk production and litter size in Egyptian Zaraibi goat

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