Plasma inorganic phosphorus concentration during eggshell formation. II. — Inverse relationships with intestinal calcium content and eggshell weight

Sauveur, Bernard; Mongin, Pierre; Antoine, Huguette

doi:10.1051/rnd:19830508

Cited by 17 publications

(9 citation statements)

References 5 publications

(5 reference statements)

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“…As the amount of dietary calcium in the eggshell increased, there was a corresponding decrease of calcium contributed from the skeleton and an improvement in shell quality based on total grams of shell. These data are also in agreement with Sauveur and Mongin (1983); their results, using variations in the concentration of plasma inorganic phosphorus during egg shell formation, also indicated an inverse relationship between bone mobilization and egg shell weight.…”

Section: Resultssupporting

confidence: 90%

Influence of Dietary Calcium on Bone Calcium Utilization

1986

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In Experiment 1, 10 microCi 45Ca/day were administered to 125 hens for 10 days. Hens were then allocated to five treatments with calcium levels ranging from .08 to 3.75% of the diet. In Experiment 2, hens with morning oviposition times were randomly allocated to 11 treatments that were periods of time postoviposition ranging from 6 hr to 24 hr, in 2-hr increments (Experiment 2). At the end of each 2-hr period, eggs from 25 hens were removed from the uterus. The 18-, 20-, and 22-hr treatments were replicated three times. In Experiment 3, hens were fed either ad libitum or feed was withheld the last 5 or 6 hr before oviposition. In Experiment 4, hens were fed 10 microCi of 45Ca for 15 days to label skeletal calcium. Hens were divided into two groups and fed a .08 or 3.75% calcium diet for 2 days. On the second day, 25 hens fed the 3.75% calcium diet were intubated with 7 g of the same diet containing .5 g calcium at 1700, 2100, 0100, 0500, and 0700 hr. The measurements used were egg weight, shell weight, and 45Ca content of the egg shell. Results indicated a significant linear or quadratic regression of dietary calcium levels on 45Ca accumulation in eggshells and eggshell weight (Experiment 1). As the calcium level of the diet increased, eggshell weight increased and 45Ca recovery decreased. Utilization of skeletal calcium for shell formation ranged from 28 to 96%. In Experiment 2, the rate of shell calcification was not constant throughout the calcification process but varied significantly.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Resultssupporting

confidence: 90%

Influence of Dietary Calcium on Bone Calcium Utilization

1986

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“…In the duodenum, CLDN2 expression increased with age. Both segments support the increase of intestinal Ca absorption during shell formation, when high contents of soluble Ca are present in the intestine (Hurwitz and Bar, 1965, 1969; Sauveur and Mongin, 1983; Guinotte et al, 1995). These segments are the main Ca absorption sites, in contrast to the ileum, and their high rate of Ca absorption may largely result from a non-saturable Ca transport, because CALB1 concentration does not vary during the daily ovulatory cycle (Hurwitz and Bar, 1966, 1968; Nys et al, 1992).…”

Section: Discussionmentioning

confidence: 76%

“…To meet this demand, the hen adapts its daily Ca intake (4.2 to 4.6 g/d) and retention, notably by optimizing its intestinal Ca absorption during the ovulatory cycle (Hurwitz and Bar, 1965; Bar, 2009). Before the dark period, laying hens exhibit a specific appetite for Ca and crop dilatation favors enhanced acid secretion in the proventriculus, increasing Ca solubilization (Mongin and Sauveur, 1974; Sauveur and Mongin, 1983; Guinotte et al, 1995). These mechanisms facilitate the intense Ca absorption during eggshell formation (Hurwitz and Bar, 1965, 1969).…”

Section: Introductionmentioning

confidence: 99%

“…Incorporation of coarse calcium carbonate into the diet can improve Ca retention by extending the supply of soluble Ca in the intestine during the dark period (Guinotte et al, 1995). When the content of soluble Ca in the intestine becomes limiting, Ca is mobilized from the medullary bone formed before the onset of laying which constitutes a labile source of Ca (Castillo et al, 1979; Sauveur and Mongin, 1983; Kerschnitzki et al, 2014). Any imbalance in Ca homeostasis impairs the quality of the eggshell or bone mineralization and strength, and Ca deficiency is the primary cause of osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

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Candidate genes of the transcellular and paracellular calcium absorption pathways in the small intestine of laying hens

et al. 2019

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To meet the high calcium (Ca) demand during eggshell biomineralization (2 g of Ca per egg), laying hens develop specific metabolic regulations to maintain Ca homeostasis. The intake of Ca, its solubilization, and absorption capacity are enhanced at sexual maturity (SM). A better knowledge of the intestinal Ca transporters involved in their variations at this stage could indicate new nutritional strategies to enhance Ca digestive utilization. Transcellular Ca absorption pathway and its major player calbindin-D 28 K (CALB1) mediate a saturable transport, which has been extensively described in this model. Conversely, a contribution by the paracellular pathway involving non-saturable Ca transport through intercellular tight junction has also been suggested. The aim of the present study was to identify candidate genes of these two pathways and their patterns of expression, in immature pullets (12, 15, and 17 wk old) and mature laying hens (23 wk old) in the duodenum, jejunum, and ileum. Using RT-qPCR, this study identifies 3 new candidate genes for transcellular, and 9 for paracellular Ca transport. A total of 5 candidates of the transcellular pathway, transient receptor potential cation channels subfamily C member 1 (TRPC1) and M member 7 (TRPM7); CALB1 and ATPase plasma membrane Ca2+ transporting 1 (ATP2B1) and ATPase plasma membrane Ca2+ transporting 2 (ATP2B2) were enhanced with age or after SM in the duodenum, the jejunum or all 3 segments. A total of 4 candidates of the paracellular pathway Claudin 2 (CLDN2) and tight junction proteins 1, 2, and 3 (TJP1, TJP2 and TJP3) increased in the small intestine after SM. Additionally, CALB1, ATP2B2, and CLDN2 were overexpressed in the duodenum or the jejunum or both segments after SM. The enhanced expression of candidate genes of the paracellular Ca pathway after SM, supports that the non-saturable transport could be a mechanism of great importance when high concentrations of soluble Ca are observed in the intestinal content during eggshell formation. Both pathways may work cooperatively in the duodenum and jejunum, the main sites of Ca absorption in laying hens.

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“…A separate provision of dietary Ca reduced this bone mobilisation at night. According to Sauveur and Mongin (1983), the higher the duodenal-soluble Ca content during egg-shell formation, the lower the bone mobilisation and phosphoraemia and the higher the eggshell deposition. These results confirmed the interest of separate Ca feeding and relatively low dietary P levels for eggshell formation.…”

Section: Calcium and Phosphorus Nutrition In Laying Hensmentioning

confidence: 99%