Plasma induced DNA damage: Comparison with the effects of ionizing radiation

Lazović, Saša; Maletic, Dejan; Leskovac, Andreja; Filipović, Jelena; Puаč, Nevena; Malović, Gordana; Joksić, Gordana; Petrović, Zoran Lj.

doi:10.1063/1.4896626

Cited by 31 publications

(20 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, the combination settings increased p53 expression, which was possibly intertwined with increased γH2AX. Additionally, radiotherapy-induced DNA damage and subsequent misrepair may lead to the formation of complex chromosomal aberrations and micronuclei (MN) that contribute to cell death [42]. For the single treatment regimens (low 3 × 2 Gy fractionated radiotherapy; plasma treatment), a significant change in the number of micronuclei was not observed.…”

Section: Discussionmentioning

confidence: 99%

Combination of Gas Plasma and Radiotherapy Has Immunostimulatory Potential and Additive Toxicity in Murine Melanoma Cells in Vitro

Pasqual-Melo

Sagwal

Freund

et al. 2020

IJMS

View full text Add to dashboard Cite

Despite continuous advances in therapy, malignant melanoma is still among the deadliest types of cancer. At the same time, owing to its high plasticity and immunogenicity, melanoma is regarded as a model tumor entity when testing new treatment approaches. Cold physical plasma is a novel anticancer tool that utilizes a plethora of reactive oxygen species (ROS) being deposited on the target cells and tissues. To test whether plasma treatment would enhance the toxicity of an established antitumor therapy, ionizing radiation, we combined both physical treatment modalities targeting B16F10 murine melanoma cell in vitro. Repeated rather than single radiotherapy, in combination with gas plasma-introduced ROS, induced apoptosis and cell cycle arrest in an additive fashion. In tendency, gas plasma treatment sensitized the cells to subsequent radiotherapy rather than the other way around. This was concomitant with increased levels of TNFα, IL6, and GM-CSF in supernatants. Murine JAWS dendritic cells cultured in these supernatants showed an increased expression of cell surface activation markers, such as MHCII and CD83. For PD-L1 and PD-L2, increased expression was observed. Our results are the first to suggest an additive therapeutic effect of gas plasma and radiotherapy, and translational tumor models are needed to develop this concept further.

show abstract

Section: Discussionmentioning

confidence: 99%

Combination of Gas Plasma and Radiotherapy Has Immunostimulatory Potential and Additive Toxicity in Murine Melanoma Cells in Vitro

Pasqual-Melo

Sagwal

Freund

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Numerous in vitro studies have proven dose-dependent plasma effects on isolated and cellular DNA. Different research groups focused on complex pathophysiological effects of CAP on isolated [29][30][31][32] and cellular [32][33][34][35] DNA. These findings, which were mainly collected in vitro, have already been partially supported by clinical data.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Risk Assessment for Medical Application of Cold Atmospheric Pressure Plasma

et al. 2020

View full text Add to dashboard Cite

Despite increasing knowledge gained based on multidisciplinary research, plasma medicine still raises various questions regarding specific effects as well as potential risks. With regard to significant statements about in vivo applicability that cannot be prognosticated exclusively based on in vitro data, there is still a deficit of clinical data. This study included a clinical follow-up of five probands who had participated five years previously in a study on the influence of cold atmospheric pressure plasma (CAP) on the wound healing of CO2 laser-induced skin lesions. The follow-up included a complex imaging diagnostic involving dermatoscopy, confocal laser scanning microscopy (CLSM) and hyperspectral imaging (HSI). Hyperspectral analysis showed no relevant microcirculatory differences between plasma-treated and non-plasma-treated areas. In summary of all the findings, no malignant changes, inflammatory reactions or pathological changes in cell architecture could be detected in the plasma-treated areas. These unique in vivo long-term data contribute to a further increase in knowledge about important safety aspects in regenerative plasma medicine. However, to confirm these findings and secure indication-specific dose recommendations, further clinical studies are required.

show abstract

“…In contrast, Kalghatgi et al [ 7 ] and Lazovic et al [ 77 ] determined that plasma-induced phosphorylation of H2AX is ATR-dependent and not ATM-dependent. In their dose-dependent study of APP treatment of MCF10A human breast epithelial cells in vitro , Kalghatgi et al [ 7 ] observed cell proliferation at low doses and apoptosis at high doses.…”

Section: App Interactions With Cellular Dnamentioning

confidence: 99%

Effects of Atmospheric Pressure Plasmas on Isolated and Cellular DNA—A Review

Arjunan

Sharma

Ptasińska

2015

IJMS

153

111

View full text Add to dashboard Cite

Atmospheric Pressure Plasma (APP) is being used widely in a variety of biomedical applications. Extensive research in the field of plasma medicine has shown the induction of DNA damage by APP in a dose-dependent manner in both prokaryotic and eukaryotic systems. Recent evidence suggests that APP-induced DNA damage shows potential benefits in many applications, such as sterilization and cancer therapy. However, in several other applications, such as wound healing and dentistry, DNA damage can be detrimental. This review reports on the extensive investigations devoted to APP interactions with DNA, with an emphasis on the critical role of reactive species in plasma-induced damage to DNA. The review consists of three main sections dedicated to fundamental knowledge of the interactions of reactive oxygen species (ROS)/reactive nitrogen species (RNS) with DNA and its components, as well as the effects of APP on isolated and cellular DNA in prokaryotes and eukaryotes.

show abstract

Plasma induced DNA damage: Comparison with the effects of ionizing radiation

Cited by 31 publications

References 38 publications

Combination of Gas Plasma and Radiotherapy Has Immunostimulatory Potential and Additive Toxicity in Murine Melanoma Cells in Vitro

Combination of Gas Plasma and Radiotherapy Has Immunostimulatory Potential and Additive Toxicity in Murine Melanoma Cells in Vitro

Long-Term Risk Assessment for Medical Application of Cold Atmospheric Pressure Plasma

Effects of Atmospheric Pressure Plasmas on Isolated and Cellular DNA—A Review

Contact Info

Product

Resources

About