Plasma heat shock protein 27 is increased in renal dysfunction and habitual smoking in a Japanese general population

Nakayoshi, Takaharu; Adachi, Hisashi; Ohbu-Murayama, Kyoko; Enomono, Mika; Fukami, Ako; Kumagai, Eita; Nakamura, Sachiko; Nohara, Yume; Nakao, Erika; Sasaki, Ken-ichiro

doi:10.1016/j.jjcc.2015.04.005

Cited by 12 publications

(7 citation statements)

References 27 publications

(42 reference statements)

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“…Associations between antibodies, HSP, CVD risk and hypertension have been documented in several studies (Kocsis et al, 2002;Pockley et al, 2002;Zhang et al, 2008). However, biomarker studies in elderly patients are limited and mostly, non-exclusive (Bårdsen et al, 2016;Galović et al, 2016;Nakayoshi et al, 2016). In this present study, we were able to observe that anti-HSP60 plasmatic presences were associated with risk factors in the Framingham Score, one of the principal measurements for CVD in elderly patients.…”

Section: Discussionsupporting

confidence: 64%

Heat Shock Protein 60 Antibodies Are Associated With a Risk Factor for Cardiovascular Disease in Bedridden Elderly Patients

Filho

Freire

Nahás

et al. 2020

Front. Mol. Biosci.

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Frailty, in elderly people, represents multiple deficiencies in different organs and is characterized by decreased physiological reserves and greater vulnerability to stressors. Bedridden elderly, with cardiovascular disease (CVD), have a worse prognosis than nonbedridden patients. Heat-shock proteins (HSPs) are molecular chaperones that under physiological conditions facilitate the transport, folding and assembly of proteins. Serum HSP 60-kDa concentrations and their antibodies are increased, in response to nonphysiological conditions, suggesting the involvement of HSPs and their antibodies in the development of CVD. The aim of this work was to evaluate heat shock protein 60 and anti-HSP60 antibody levels, associated with a risk factor for cardiovascular disease, in bedridden elderly patients. Clinical, analytical and cross-sectional analyses were performed with 57 elderly (>65 years). HSP60 and anti-HSP60 plasma levels were measured by ELISA. Bivariate analysis using a linear regression model adjusted for risk factors used Framingham Score. Among the 57 elderly, with an average age of 69.89 years, 39% are bedridden; 26% with pre-existing cardiovascular disease and 44% are dyslipidemic. The relationship of risk factors in the Framingham Score was positive for the anti-HSP60 antibody (p = 0.042) measurement. Our data show a positive correlation among the elevation of the Framingham score and the profile of anti-HSP60 antibodies. These results suggest a greater immune activation that is associated with cardiovascular risk and bedridden fragility.

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Section: Discussionsupporting

confidence: 64%

Heat Shock Protein 60 Antibodies Are Associated With a Risk Factor for Cardiovascular Disease in Bedridden Elderly Patients

Filho

Freire

Nahás

et al. 2020

Front. Mol. Biosci.

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“…Regarding the relationship between HSP27 and smoking, it has recently been shown that the plasma levels of HSP27 are higher in habitual smokers than in non-smokers and that the levels of plasma HSP27 in former smokers are almost within the normal range (35). Based on these present and previous findings, it seems that the temporary enhancement of phosphorylated-HSP27 release from human platelets after smoking cessation might be implicated in the deterioration of atherosclerotic pathophysiology through modulating inflammation progress.…”

Section: Discussionsupporting

confidence: 60%

Cigarette Smoking Cessation Temporarily Enhances the Release of Phosphorylated-HSP27 from Human Platelets

et al. 2020

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Objective Cigarette smoking is a risk factor for arteriopathy, including acute coronary syndrome, stroke and peripheral vascular disease. Thus, cessation is strongly recommended in order to reduce these risks. We recently demonstrated that smoking cessation causes temporary hyper-aggregability of human platelets. We previously showed that heat shock protein 27 (HSP27) is released from human platelets stimulated by collagen, accompanied by its phosphorylation. Accumulating evidence indicates potent roles of extracellular HSP27 as a modulator of inflammation. In the present study, using the stored samples obtained in the previous study, we investigated the effect of cigarette smoking cessation on the release of phosphorylated-HSP27 from collagen-activated human platelets (n=15 patients). Methods We enrolled patients who visited smoking cessation outpatient services between January 2012 and November 2014. Platelet-rich plasma, chronologically obtained before and after the cessation, was stimulated by collagen using a PA-200 aggregometer in the previous study. The levels of phosphorylated-HSP27 released from platelets were determined by an enzyme-linked immunosorbent assay. The phosphorylation of HSP27 in platelets was evaluated by a Western blot analysis. Results Cessation of cigarette smoking significantly upregulated the levels of collagen-stimulated release of phosphorylated-HSP27 at four and eight weeks after quitting smoking compared to before cessation. However, there was no significant difference between the levels before cessation and those at 12 weeks after cessation. The levels of phosphorylated-HSP27 stimulated by collagen in the platelets at four weeks after smoking cessation were remarkably enhanced compared to before cessation. Conclusion Cigarette smoking cessation temporarily enhances the collagen-stimulated release of phosphorylated-HSP27 from human platelets in the short term.

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“…Cigarette smoking is one of the most important causes of cardiovascular diseases acting via oxidative stress, [12] in which it has been demonstrated that smoking increases arterial stiffness, independent of blood pressure (BP). [13,14] Interestingly, smoking cessation more than a decade has been reported to recover arterial stiffness parameters to nonsignificant levels, [13] because smoking cessation reduces the levels of inflammatory markers such as C-reactive protein and fibrinogen.…”

Section: Introductionmentioning

confidence: 99%

Augmentation index (AI) in a dose–response relationship with smoking habits in males

Tsuru

Adachi²,

Enomoto³

et al. 2016

Medicine

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We investigated the relationship between augmentation index (AI) and smoking habits in community-dwelling Japanese.This cross-sectional study enrolled 1926 subjects (769 males and 1157 females) aged 40 to 95 years who underwent a health check-up in a Japanese cohort of the Seven Countries Study, in Tanushimaru, a typical farming town in Kyushu Island in 2009. The subjects’ medical history, alcohol intake, smoking habit, and current medications for hypertension, dyslipidemia, and diabetes were ascertained by questionnaire. Radial arterial pressure wave analysis was used to obtain AI. We analyzed the data stratified by gender.Age-adjusted means of AI in males showed a clear dose–response relationship in 4 categories of smoking habits (P = 0.010). There was no significant relationship between AI and smoking habits in females (P = 0.127). The significant dose–response relationship (P = 0.036) in males between AI and 4 categories of smoking habits still remained even after adjustment for age, body mass index, systolic blood pressure, estimated glomerular filtration rate, glucose, hypertensive medication, and alcohol intake.The present study demonstrated that AI values were significantly associated with smoking habits in a dose-dependent manner in Japanese males.

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Plasma heat shock protein 27 is increased in renal dysfunction and habitual smoking in a Japanese general population

Abstract: The present study demonstrated that HSP 27 levels were strongly related to renal dysfunction and habitual smoking in a dose-response manner in a Japanese general population.

Cited by 12 publications

References 27 publications

Heat Shock Protein 60 Antibodies Are Associated With a Risk Factor for Cardiovascular Disease in Bedridden Elderly Patients

Heat Shock Protein 60 Antibodies Are Associated With a Risk Factor for Cardiovascular Disease in Bedridden Elderly Patients

Cigarette Smoking Cessation Temporarily Enhances the Release of Phosphorylated-HSP27 from Human Platelets

Augmentation index (AI) in a dose–response relationship with smoking habits in males

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