Plasma free amino acids and their metabolites in Taiwanese patients on hemodialysis and continuous ambulatory peritoneal dialysis

Chen, Chuang; Lin, Shuan‐Pei; Chen, Han‐Hsiang; Chen, Yi-Chou; Wang, Tuan-Jen; Shieh, Wen-Hsiang; Wu, Chih‐Jen

doi:10.1016/j.cccn.2005.07.001

Cited by 26 publications

(25 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pipecolic acid was the least abundant. These normal mean levels of AAs in plasma were similar to those values reported in the literature (Slocum and Cummings, 1991;Rashed et al, 2001;Chuang et al, 2006).…”

Section: Levels Of Amino Acids In Pooled Normal Plasmasupporting

confidence: 89%

Target metabolic profiling analysis of free amino acids in plasma as EOC/TBDMS derivatives by GC‐SIM‐MS

Paik

Moon

Kim

et al. 2007

Biomedical Chromatography

View full text Add to dashboard Cite

Metabolic profiling analysis of free amino acids (AAs) in plasma (20 microL) was performed by gas chromatography-mass spectrometry in selected ion monitoring mode after ethoxycarbonyl/tert-butyldimethylsilyl derivatives. Characteristic fragment ions, including [M - 57](+) ions, permitted sensitive and selective detection of most of the AAs in the presence of co-extracted carboxylic acids, including free fatty acids, at much higher levels. The overall method was linear (r > or = 0.9991), reproducible (relative standard deviation = 2.3-8.8%) and accurate (relative error = -7.3-7.7%) with detection limits of 0.01-1.9 ng/mL. A total of 18 AAs, 15 protein AAs and three nonprotein AAs were quantitatively screened in a normal human plasma sample. This selective and simple method using a minimal sample volume was effective for the quantitation of plasma free AAs.

show abstract

Section: Levels Of Amino Acids In Pooled Normal Plasmasupporting

confidence: 89%

Target metabolic profiling analysis of free amino acids in plasma as EOC/TBDMS derivatives by GC‐SIM‐MS

Paik

Moon

Kim

et al. 2007

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…The cellular and plasma amino acid profile in chronic renal insufficiency patients is altered, as a result of low-protein diet, distorted protein and amino acid catabolism, and disturbed interorgan transport [8,9]. Total content of all amino acids in plasma of ESRF patients is most often higher than in healthy persons.…”

Section: Discussionmentioning

confidence: 99%

Total, free, and protein-bound thiols in plasma of peritoneal dialysis and predialysis patients

Przemysław¹,

Ksiazek

Chwatko

et al. 2011

Int Urol Nephrol

View full text Add to dashboard Cite

Thiol compounds such as glutathione, homocysteine, and cysteinyl-glycine are the natural reservoir of reductive capacity of the cells. Chronic renal failure is accompanied by disturbances in redox status of plasma thiols. The aim of the present study was to compare the changes in concentrations of different forms of thiols in plasma of terminal renal failure patients, nondialyzed and on peritoneal dialysis. Total concentrations of different redox forms of thiols were determined by high performance liquid chromatography. We observed that total concentration of glutathione in terminal renal failure patients decreased and total concentration of the remaining thiols in these patients significantly increased. Continuous ambulatory peritoneal dialysis had the following features in comparison with nondialyzed patients: (1) glutathione and cysteine concentration was restored and (2) free fraction of thiols rose, while protein-bound fraction dropped (except for homocysteine). Continuous ambulatory peritoneal dialysis corrects total concentration of glutathione and cysteine, in comparison with nondialyzed patients.

show abstract

“…In recent years, the importance of nutritional status in patients with chronic renal or liver disease has been emphasized by studies that relate malnutrition with increased morbidity and mortality. Thus, nutritional status as assessed by plasma concentrations of amino acids in predialysis patients has been considered to be a predictor for prognosis in the first period of dialysis (8)(9)(10). Also, nutritional related imbalances of free plasma amino acids before transplant surgery in patients with chronic liver disease have been associated with a higher rate of complications and a decreased overall survival rate following transplantation (11,12).…”

Section: Introductionmentioning

confidence: 99%

Biological variation of free plasma amino acids in healthy individuals

Corte

Venta²

2009

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Background: Biological variation data for free plasma amino acids are lacking from the more comprehensive databases. Therefore, we determined the intra-and inter-individual components of variation in healthy subjects. These data were used to define desirable goals for imprecision, bias and total error, indices of individuality and reference change values. Methods: Plasma samples were collected from 11 volunteers at weekly intervals over 5 weeks. Free plasma amino acids were analyzed by reversed-phase HPLC and analytical and biological variation data were derived using ANOVA. Results: Intra-individual coefficients of variation ranged from 9.5% to 46.4%, with lower values among the essential amino acids. The mean inter-individual coefficient of variation was 46.6% and was higher than intra-individual values for all amino acids. Thus, indices of individuality were below 0.8. Reference change values ranged from 30.9% to 128.4% and total error values ranged from 15.2% to 61.0%. Conclusions: Plasma amino acids exhibit relatively low intra-individual coefficients of variation, with essential amino acids showing tighter homeostatic control. Analytical quality goals can be reasonably achieved with current methods. Reference intervals are of limited value in the detection of unusual results in an individual. Therefore, comparison of serial results by means of the reference change values is recommended. Clin Chem Lab Med 2010;48:99-104.

show abstract

Plasma free amino acids and their metabolites in Taiwanese patients on hemodialysis and continuous ambulatory peritoneal dialysis

Cited by 26 publications

References 33 publications

Target metabolic profiling analysis of free amino acids in plasma as EOC/TBDMS derivatives by GC‐SIM‐MS

Target metabolic profiling analysis of free amino acids in plasma as EOC/TBDMS derivatives by GC‐SIM‐MS

Total, free, and protein-bound thiols in plasma of peritoneal dialysis and predialysis patients

Biological variation of free plasma amino acids in healthy individuals

Contact Info

Product

Resources

About