Plasma exosomes from endometrial cancer patients contain LGALS3BP to promote endometrial cancer progression

Song, Yiran; Wang, Mengfei; Tong, Huan; Tan, Yuan; Hu, Xiang; Wang, Kai; Wan, Xiaoping

doi:10.1038/s41388-020-01555-x

Cited by 60 publications

(46 citation statements)

References 33 publications

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“…Among the top 10 enriched proteins in exosomes (colored red in Figure 1C), we found five endosomal pathway regulators (PDCD6IP, SDCBP, ANXA11, TSG101, and VPS37B) which are pivotal for exosome biogenesis, and CD63, a widely known exosomal tetraspanin marker. LGALS3BP has been found in diverse cancer-derived exosomes (Escrevente et al, 2013;Ung et al, 2014;Dutta et al, 2015;Gomes et al, 2015;Song et al, 2021), and APOE has been found in exosomes released from macrophages (Zheng et al, 2018) and neurons (Nikitidou et al, 2017;Mathews and Levy, 2019;Peng et al, 2019). This comparative analysis shows that our classification pipeline can successfully categorize proteomic datasets and suggests that systematic analysis of published proteomic studies is a valuable approach for discovering novel markers for ectosome and exosomes.…”

Section: Small Extracellular Vesicle and Large Extracellular Vesicle Datasets Exhibit A Differential Separation Of Exosomal And Ectosomalmentioning

confidence: 77%

Extracellular Vesicle Proteomes Shed Light on the Evolutionary, Interactive, and Functional Divergence of Their Biogenesis Mechanisms

Lim

Yoon

Kim

et al. 2021

Front. Cell Dev. Biol.

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Extracellular vesicles (EVs) are membranous structures containing bioactive molecules, secreted by most cells into the extracellular environment. EVs are classified by their biogenesis mechanisms into two major subtypes: ectosomes (enriched in large EVs; lEVs), budding directly from the plasma membrane, which is common in both prokaryotes and eukaryotes, and exosomes (enriched in small EVs; sEVs) generated through the multivesicular bodies via the endomembrane system, which is unique to eukaryotes. Even though recent proteomic analyses have identified key proteins associated with EV subtypes, there has been no systematic analysis, thus far, to support the general validity and utility of current EV subtype separation methods, still largely dependent on physical properties, such as vesicular size and sedimentation. Here, we classified human EV proteomic datasets into two main categories based on distinct centrifugation protocols commonly used for isolating sEV or lEV fractions. We found characteristic, evolutionarily conserved profiles of sEV and lEV proteins linked to their respective biogenetic origins. This may suggest that the evolutionary trajectory of vesicular proteins may result in a membership bias toward specific EV subtypes. Protein–protein interaction (PPI) network analysis showed that vesicular proteins formed distinct clusters with proteins in the same EV fraction, providing evidence for the existence of EV subtype-specific protein recruiters. Moreover, we identified functional modules enriched in each fraction, including multivesicular body sorting for sEV, and mitochondria cellular respiration for lEV proteins. Our analysis successfully captured novel features of EVs embedded in heterogeneous proteomics studies and suggests specific protein markers and signatures to be used as quality controllers in the isolation procedure for subtype-enriched EV fractions.

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Section: Small Extracellular Vesicle and Large Extracellular Vesicle Datasets Exhibit A Differential Separation Of Exosomal And Ectosomalmentioning

confidence: 77%

Extracellular Vesicle Proteomes Shed Light on the Evolutionary, Interactive, and Functional Divergence of Their Biogenesis Mechanisms

Lim

Yoon

Kim

et al. 2021

Front. Cell Dev. Biol.

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“…Lectin Galactoside-Binding Soluble 3 Binding Protein Elevated levels of lectin galactoside-binding soluble 3 binding protein (LGALS3BP) in serum or tumor tissue are known to be associated with shorter survival in patients with breast, non-small cell lung, and endometrial cancers, lymphoma, and malignant pleural mesothelioma [59,60]. Song et al [60] recently found that LGALS3BP in plasma exosomes from patients with endometrial cancer induces tube formation and that exosomes containing LGALS3BP overexpressed in human embryonic kidney 293 cells upregulate VEGF expression, in human endothelial cell lines and primary endothelial cells.…”

Section: Galectin-1mentioning

confidence: 99%

VEGF-Independent Angiogenic Factors: Beyond VEGF/VEGFR2 Signaling

2022

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Tumors induce angiogenesis to acquire oxygen and nutrition from their adjacent microenvironment. Tumor angiogenesis has been believed to be induced primarily by the secretion of vascular endothelial growth factor-A (VEGF-A) from various tumors. VEGF-A binds to VEGF receptor 2 (VEGFR2), resulting in subsequent activation of cellular substances regulating cell proliferation, survival, and angiogenesis. Antiangiogenic therapies targeting the VEGF-A/VEGFR2 axis, including bevacizumab and ramucirumab, humanized monoclonal antibodies against VEGF-A and VEGFR2, respectively, have been proposed as a promising strategy aimed at preventing tumor growth, invasion, and metastasis. Phase III clinical trials using bevacizumab and ramucirumab have shown that not all tumor patients benefit from such antiangiogenic agents, and that some patients who initially benefit subsequently become less responsive to these antibodies, suggesting the possible existence of VEGF-independent angiogenic factors. In this review, we focus on VEGF-independent and VEGFR2-dependent tumor angiogenesis, as well as VEGFR2-independent tumor angiogenesis. Additionally, we discuss VEGF-independent angiogenic factors which have been reported in previous studies. Various molecular targeting drugs are currently being evaluated as potential antitumor therapies. We expect that precision medicine will permit the development of innovative antiangiogenic therapies targeting individual angiogenic factors selected on the basis of the genetic screening of tumors.

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“… 145 Exosomal lectin galactoside-binding soluble 3-binding protein (LGALS3BP) was detected in exosomes isolated from the plasma of endometrial cancer patients and was upregulated with endometrial cancer progression, promoted the growth of endometrial cancer cells and facilitated the angiogenesis of HUVECs. 146 The nucleic acids and proteins carried by exosomes may be involved in the progression of endometrial cancer.…”

Section: The Role Of Exosomes In Endometrial Cancermentioning

confidence: 99%

Nucleic acids and proteins carried by exosomes of different origins as potential biomarkers for gynecologic cancers

Wang

Pan

Zheng

et al. 2022

Molecular Therapy - Oncolytics

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Exosomes are extracellular vesicles with a diameter of 30–150 nm that function in mediating intercellular communication and intercellular material exchange. The liposomal membrane of exosomes protects the cargo carried by exosomes from degradation and assists in transporting cargo to recipient cells to regulate a variety of physiological and pathological processes. The incidence of gynecologic cancers is increasing annually, which is extremely harmful to the lives and health of women because such cancers are challenging to detect at the early stage. Recently, exosomes have emerged as novel biomarkers for diagnosing and predicting the development of gynecologic cancers. In particular, non-coding RNAs (microRNAs [miRNAs], long non-coding RNAs [lncRNAs], and circular RNAs [circRNAs]) carried by exosomes have been extensively investigated in gynecologic cancers. Therefore, the purpose of this review is to focus on the potential roles of exosomes of different origins in ovarian cancer, cervical cancer, and endometrial cancer, which will help to determine the molecular mechanism of carcinogenesis.

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Plasma exosomes from endometrial cancer patients contain LGALS3BP to promote endometrial cancer progression

Cited by 60 publications

References 33 publications

Extracellular Vesicle Proteomes Shed Light on the Evolutionary, Interactive, and Functional Divergence of Their Biogenesis Mechanisms

Extracellular Vesicle Proteomes Shed Light on the Evolutionary, Interactive, and Functional Divergence of Their Biogenesis Mechanisms

VEGF-Independent Angiogenic Factors: Beyond VEGF/VEGFR2 Signaling

Nucleic acids and proteins carried by exosomes of different origins as potential biomarkers for gynecologic cancers

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