Plasma DNA as a marker of cancerous cell death. Investigations in patients suffering from lung cancer and in nude mice bearing human tumours

Luteal phase serum cell-free DNA as a marker of failed pregnancy after assisted reproductive technology

“…This suggested that the previously reported embryotoxic serum factors (11)(12) were not associated with cellular processes that generated the cell-free DNA. Furthermore, the finding of a lack of difference suggested that cell-free DNA concentrations at the 1-week post transfer period could not be used as a marker for failed pregnancies (16). Note that the present study focused on cell-free DNA of damaged cells originating from maternal tissues and not from the transferred embryos.…”

“…Circulating extracellular DNA have been detected in both healthy and sick individuals (13) and are present in large concentrations in cancer patients due to apoptosis (14)(15)(16) and active secretion (17).…”

Quantification of free total plasma DNA and minimal residual disease detection in the plasma of children with acute lymphoblastic leukemia

“…It is widely known that a substantial, if not predominant (depending on the course of therapy) portion of plasma DNA in cancer patients derives from tumor cells [27]. Higher plasma concentrations have been described in patients with large tumors or at an advanced stage of the disease [2,3,9,28,29] and it has been postulated that circulating plasma DNA in these disease states originates largely from tumor lysis/ m Male, f female, good less than 1,000 leukemic blood blasts on treatment day 8, poor more than 1,000 leukemic blood blasts, SR negative at days 33 and 78, HR still 10 −3 or more at day 78, MR all others, TEL/AML1 fusion transcript positivity, none of the patients was positive for the BCR/ ABL fusion transcript or an MLL aberration necrosis and apoptosis. In 2001, Jahr et al described a significant increase of plasma DNA after induction of apoptosis and necrosis in both, a cell culture and a murine model system, with the increase being more impressive in the necrosis model [30].…”

Detection of cell free Epstein–Barr virus DNA in sera from patients with nasopharyngeal carcinoma

“…15 The source of cell free EBV DNA in circulation of patients with EBV-harboring malignancy remains somewhat controversial. Intact EBV particles 10,16,17 or free EBV DNA molecules released through the process of tumor death 9,12,18 both may exist in the circulation of patients with active disease. Conversely, in contrast to previous reports 9,10 suggesting that no cell free EBV could be detected in serum samples from patients with NPC in clinical remission, we were able to detect cell free EBV DNA in 36.5% of serum samples derived from patients with NPC who were in remission, and occasional (7.1%) high EBV DNA copy numbers (as indicated by positive results from 35-cycle PCR) were noted.…”