Luteal phase serum cell-free DNA as a marker of failed pregnancy after assisted reproductive technology

Hart, Elaine; Patton, William C.; Jacobson, John D.; King, Alan; Corselli, J.; Chan, Philip J.

doi:10.1007/s10815-005-4924-4

Cited by 8 publications

(5 citation statements)

References 26 publications

(34 reference statements)

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“…This study found that women who did not conceive during the IVF cycle had statistically significantly higher mean concentrations of cfDNA 14 days after embryo transfer, during the bHCG blood test, compared with women who did conceive. A previous study did not find a statistically significant difference in cfDNA concentrations comparing women who conceived in IVF treatment and those who did not (Hart et al, 2005). The difference between that study and the current study could be explained in two ways.…”

Section: Discussioncontrasting

confidence: 93%

“…The difference between that study and the current study could be explained in two ways. First, the study by Hart et al (2005) was conducted using capillary electrophoresis to measure cfDNA concentrations, a method which may be less sensitive in identifying fragments of cfDNA; as described, the minimal cfDNA molecular weight was 1 Kb. Second, the determination of cfDNA by Hart et al (2005) was 1 week after embryo transfer, rather than 2 weeks in the current study.…”

Section: Discussionmentioning

confidence: 99%

“…The finding of embryo-toxic factors in the bloodstream of patients with repeated IVF failure and repeated spontaneous abortions led researchers to examine cfDNA concentrations in women undergoing IVF treatment utilizing capillary electrophoresis in serum taken 1 week after embryo transfer (Hart et al, 2005), but no relationship between cfDNA concentrations in sera and conception rates in IVF patients was found. Therefore, the current study examined cfDNA concentrations in the plasma of peripheral blood of women undergoing IVF as a possible predictive factor affecting conception rates in IVF-embryo transfer treatment.…”

Section: Introductionmentioning

confidence: 99%

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Increased plasma cell-free DNA is associated with low pregnancy rates among women undergoing IVF–embryo transfer

Czamanski-Cohen

Sarid

Cwikel

et al. 2013

Reproductive BioMedicine Online

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This prospective repeated measures study was designed to examine the cell-free DNA (cfDNA) concentrations during ovarian stimulation and the relationship between cfDNA concentration and pregnancy rates in women undergoing IVF-embryo transfer. The study examined 37 women undergoing IVF treatment in an IVF unit in a university medical centre in southern Israel. cfDNA concentrations were measured by a direct fluorescence assay, pregnancy rates were identified by plasma β human chorionic gonadotrophin (HCG) concentrations and verified by vaginal ultrasound to determine gestational sac and fetal heart beats. Throughout the IVF cycle, at the three time points measured, the mean concentration of plasma cfDNA among all participants did not statistically significantly change. However, on the day of βHCG test in patients undergoing IVF-embryo transfer, plasma cfDNA concentrations were statistically significantly higher among women who did not conceive in comparison to those who conceived. Plasma cfDNA may reflect the presence of factors which interfere with embryo implantation. Further research is required to determine the usefulness of cfDNA as a biomarker of IVF outcome and to examine the underlying pathologies as potential sources for increased plasma cfDNA concentrations. Cell-free DNA (cfDNA) is particles of DNA which are released from the cell nucleus and are found in high concentrations during a variety of illnesses and injuries. This study was designed to examine the cfDNA concentrations during IVF treatment and the relationship between cfDNA concentration in the bloodstream and pregnancy rates in women undergoing IVF. This study examined 37 women in treatment at the IVF unit of the University Medical Centre in southern Israel. cfDNA concentrations in the bloodstream were measured at three time points by a direct test. Pregnancy rates were identified by pregnancy hormone concentrations in the bloodstream and verified by vaginal ultrasound to determine a pregnancy sac and fetal heart beats. Throughout the IVF cycle, at the three time points measured, the average concentration of cfDNA among all participants did not change. However, on the day of the pregnancy test, blood cfDNA concentrations were significantly higher among women who were not pregnant in comparison to those who were. Plasma cfDNA may reflect the presence of factors which interfere with embryo implantation. Further research is required to determine the usefulness of cfDNA as a biological marker of IVF outcome and examine underlying illnesses and problems as potentials sources for increased cfDNA concentrations.

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Section: Discussioncontrasting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Increased plasma cell-free DNA is associated with low pregnancy rates among women undergoing IVF–embryo transfer

Czamanski-Cohen

Sarid

Cwikel

et al. 2013

Reproductive BioMedicine Online

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“…However, no relationship was found between CFD levels in sera and conception rates in IVF patients in serum taken 1 week after embryo transfer [15].…”

Section: Cell-free Dnamentioning

confidence: 85%

Cell-free DNA and telomere length among women undergoing in vitro fertilization treatment

Czamanski-Cohen

Sarid

Cwikel

et al. 2015

J Assist Reprod Genet

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Purpose The current research is aimed at finding potential non-invasive bio-markers that will help us learn more about the mechanisms at play in failed assisted reproduction treatment. This exploratory pilot study examined the relationship between cell-free DNA (CFD) in plasma and telomere length in lymphocytes among women undergoing in vitro fertilization (IVF) and compared telomere length and CFD levels to a healthy control group. Methods Blood of 20 women undergoing IVF was collected at three time points during the IVF cycle. We assessed the relationship between CFD and telomere length as well as controlling for morning cortisol levels. We also collected blood of 10 healthy controls at two time points (luteal and follicular phases of the menstrual cycle) and compared mean telomere length, CFD, and cortisol levels between the IVF patients and healthy controls.Results The results revealed an inverse relationship between CFD levels and telomere lengths at several time points that remained significant even after controlling for cortisol levels. Women undergoing IVF had statistically significant higher levels of CFD and shorter telomeres compared to healthy controls. Conclusions The relationship between telomere length and CFD should be further explored in larger studies in order to uncover potential mechanisms that cause both shortened telomere length and elevated CFD in women undergoing IVF.

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“…Serum cf-DNA has been proposed as a marker of semen quality, since it has been associated with important sperm parameters linked to normal sperm function [ 7 ]. It has been reported that there is no relationship between serum cf-DNA concentrations and IVF outcome in patients undergoing IVF-ET treatments [ 8 ], while a more recent report indicated that increased serum cell-free DNA is associated with low pregnancy rate in women undergoing IVF-ET programs [ 9 ]. Methods used for the analysis of serum cf-DNA are real-time polymerase chain reaction (PCR) [ 3 , 10 ] and quantitative PCR [ 2 ], while relative recently a new simple and highly sensitive method known as the SYBR gold fluorescent-stained direct assay has been developed [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Follicular Fluid Oocyte/Cumulus-Free DNA Concentrations as a Potential Biomolecular Marker of Embryo Quality and IVF Outcome

Dimopoulou

Anifandis

Messini

et al. 2014

BioMed Research International

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The present prospective study examined the follicular fluid oocyte/cumulus-free DNA concentrations (ff o/c-free DNA) during ovarian stimulation and the possible association between ff o/c-free DNA and embryological results such as embryo quality and pregnancy rate. Eighty-three women undergoing IV/ICSI-ET treatments were prospectively included in this study. ff o/c-free DNA was determined by conventional quantitative real time PCR-Sybr green detection approach. The 83 ff samples were categorized in two groups: group 1 (n = 62) with cumulus oocytes complexes (CoCs) ≥2 and group 2 (n = 21) with CoCs = 1. Group 1 revealed significant higher embryo quality in terms of mean score of embryo transfer (MSET), but lower ff o/c-free DNA concentrations compared to group 2. The two groups showed comparable pregnancy rates (positive hCG and clinical pregnancy). The higher the ff o/c-free DNA concentration, the lower the number of produced oocytes. ff o/c-free DNA did not seem to have any direct role in the IVF outcome. Further research is required to clarify whether ff o/c-free DNA is a biomolecular marker of embryo quality and IVF outcome.

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Luteal phase serum cell-free DNA as a marker of failed pregnancy after assisted reproductive technology

Cited by 8 publications

References 26 publications

Increased plasma cell-free DNA is associated with low pregnancy rates among women undergoing IVF–embryo transfer

Increased plasma cell-free DNA is associated with low pregnancy rates among women undergoing IVF–embryo transfer

Cell-free DNA and telomere length among women undergoing in vitro fertilization treatment

Follicular Fluid Oocyte/Cumulus-Free DNA Concentrations as a Potential Biomolecular Marker of Embryo Quality and IVF Outcome

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