Plasma Catalase in Relation to Pain Following Midline Laparotomy: A Prospective Study of Patients with Benign Diseases and Patients with Cancer

Saimanen, Iina; Kärkkäinen, Jari; Selander, Tuomas; Purdy, Martin; Kokki, Merja; Kokki, Hannu; Eskelinen, Matti

doi:10.21873/anticanres.13011

Cited by 6 publications

(8 citation statements)

References 8 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The plasma CAT assays were performed using enzyme-linked immunosorbent assay (ELISA) Kit (SEC418Hu, Cloud-Clone Corporation, USA). The study protocol of CAT was fully described in our earlier original works (7,12). The plasma NT assays were performed using competitive enzyme immunoassay OxiSelect™ Nitrotyrosine ELISA Kit (STA-305, Cell Biolabs Inc, USA).…”

Section: Methodsmentioning

confidence: 99%

Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer Patients

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background/Aim: The aim of this study was to assess the plasma concentration of the nitrosative stress biomarker nitrotyrosine (NT) in gallstone disease and cancer patients. Materials and Methods: Initially, 114 patients with symptomatic gallstone disease were randomized into the laparoscopic cholecystectomy (LC) (n=54) and the minicholecystectomy (MC) (n=60) groups. The plasma concentrations of NT were measured just before, immediately after (POP1) and 6 h after operation (POP2). The cancer patients of this study included ten patients with gastrointestinal cancer and 19 patients with gynecological cancer. Results: There was a statistically significant correlation in the median plasma NT concentrations versus plasma catalase (CAT) concentrations in cholecystectomy patients (r=0.169, p=0.001). Interestingly, there was a statistically significant inverse correlation between the individual values of the pain assessed and filed using a 11-point numeric rating scale 8 h postoperatively (NAD 8) and plasma NT median values in cholecystectomy patients (r=-0.337, p=0.004). Conclusion: Patients with high plasma concentrations of NT appeared to have significantly lower pain scores 8 h postoperatively.

show abstract

Section: Methodsmentioning

confidence: 99%

Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer Patients

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, the postoperative alteration (PRE versus POP1) of MDA concentrations in all groups combined (all patients) was statistically significant in the single dose group had a trend for higher plasma NT concentrations preoperatively (PRE) and immediately following surgery (POP1) compared to other study groups. We have earlier reported that RSB analgesia does significantly alter the oxidative stress markers SOD1/CAT concentrations (increase) and NT concentrations (decrease) immediately after operation, but SOD1/CAT marker concentrations drops back 24 h following surgery (8)(9)(10)18). Interestingly, in the present study we found that the time effect in LME analysis in plasma NT biomarker both in the single group patients and in benign patients group was statistically significant (p=0.001 and p=0.013, respectively).…”

Section: Discussionmentioning

confidence: 90%

The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background/Aim: The aim of the study was to compare the MDA (malonidialdehyde) plasma concentrations versus CAT (catalase)/NT (nitrotyrosine) plasma concentrations, patient satisfaction and pain score at rest/pressure to the wound area in laparotomy patients with rectus sheath block (RSB) analgesia. Patients and Methods: Initially, 56 patients were randomized to four groups; control group (n=12), single-dose (n=16), repeated-dose (n=12) and continuous infusion (n=16) RSB analgesia groups. The plasma concentrations of CAT, NT and MDA markers were measured just before, immediately after and 24 h after operation. Results: The RSB analgesia enhanced significantly patient satisfaction (p=0.001). The plasma MDA decreased immediately after operation (POP1) and the postoperative decrease between the preoperative and the POP1 values in the MDA marker were statistically significant (p<0.001). In linear mixed model, the time effect in both the single group and in the benign group in plasma NT biomarker was statistically significant (p=0.001, p=0.013, respectively). The median plasma MDA concentrations (ng/ml) following surgery were significantly lower in patients with cancer versus patients with benign disease (589 vs. 852, p=0.021). Jitterplots of the individual plasma NT versus plasma MDA showed that there was significant correlation in benign and cancer patients (r=0.347, p<0.001). Conclusion: Plasma MDA decreased significantly after operation in all patients and cancer patients had significantly lower MDA concentrations following surgery than patients with benign disease.Reactive oxygen species (ROS) degrade polyunsaturated fatty acids (PUFAs) by lipid peroxidation forming malonidialdehyde (MDA) (1). MDA is one of the many ROS that cause oxidative stress in cells attacking the subcellular structures and forming covalent protein adducts (1). The production and plasma concentration of MDA can be measured and used as a biomarker to estimate the degree of oxidative stress and lipid peroxidation in human disease (2). MDA is a toxic substance reacting with deoxyadenosine in DNA and forming DNA adducts, which are mutagenic (3). A schematic representation of the relationship among the formation of ROS/Reactive nitrogen species (RNS) lipid peroxidation, nitrotyrosine (NT), catalase (CAT) and MDA is shown in five recent reviews (1,(4)(5)(6)(7).Our previous study showed that the control group of patients and the rectus sheath block (RSB) groups did not differ in individual pain scores (numeric rating scales, NRS) following surgery (8, 9). However, it seems that plasma CAT levels correlate NRS pain scores following surgery (10) and NT concentration does significantly correlate satisfaction for analgesia (9). There are seven large reviews in the literature available to compare ROS/RNS in clinical trials that investigated the impact of 253 *These Authors contributed equally to this study.

show abstract

“…EDTA-blood samples were taken before surgery (PRE), immediately after surgery (POP1) and 6 h post-operatively (POP2) and centrifuged at 1,000G (2,900 rpm) for 15 min. The study protocol of CAT and NT were fully described in our earlier original works (4,5,13,14). Plasma MDA concentrations were determined by using MDA (Malondialdehyde) ELISA Kit (E-EL-0060, Elabscience, Biotechnology Inc. Houston, Texas, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The harmful effects of MDA could be neutralized by substances known as antioxidants such as vitamin C and vitamin E, which may inhibit lipid peroxidation. Catalase (CAT) is one antioxidative substance, which plasma concentrations have shown to have inverse correlation to analgesic doses during the first 24 hours following surgery (4) and an inverse correlation to numeric rating scale (NRS) pain scores following surgery in midline laparotomy patients (5). Our previous article showed that the cancer patients with midline laparotomy had significantly lower plasma MDA concentrations postoperatively, whereas the postoperative alteration of median plasma CAT levels in cancer patients were significantly higher in the benign group of patients (6).…”

mentioning

confidence: 99%

The Induction of Antioxidant Catalase Enzyme With Decrease of Plasma Malonidialdehyde: An Important Reactive Oxidative Species Inhibiting Mechanism

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background/Aim: The simultaneous increase of antioxidant CAT (catalase) enzyme and plasma MDA (malonidialdehyde) concentrations versus the numeric rating scale (NRS) pain score following surgery is unknown. Patients and Methods: The study included 114 patients with gallstone disease and 29 patients in the cancer group. Results: Following surgery, the plasma CAT concentrations increased and plasma MDA concentrations decreased in all patients and especially in cancer patients. The linear mixed model time-effect was statistically significant in CAT and MDA (p<0.001 and p=0.02, respectively). In addition, a significant correlation between NRS pain score values and plasma MDA median concentrations in cancer patients was identified (r=0.430, p<0.001). Conclusion: The plasma MDA concentrations decreased and CAT concentrations increased significantly in all patients and especially in cancer patients following surgery. The simultaneous increase of antioxidant CAT enzyme with the decrease of plasma MDA may be an important ROS inhibiting mechanism to help patients return to normal antioxidant-oxidant status. Lipid peroxidation is a process where reactive oxygen species (ROS) attack lipids containing carbon double bonds (C-C), 5701 This article is freely accessible online.

show abstract

Plasma Catalase in Relation to Pain Following Midline Laparotomy: A Prospective Study of Patients with Benign Diseases and Patients with Cancer

Cited by 6 publications

References 8 publications

Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer Patients

Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer Patients

The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer

The Induction of Antioxidant Catalase Enzyme With Decrease of Plasma Malonidialdehyde: An Important Reactive Oxidative Species Inhibiting Mechanism

Contact Info

Product

Resources

About