Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer Patients

Saimanen, Iina; Rahkola, Dina; Kuosmanen, Viivi; Kärkkäinen, Jari; Selander, Tuomas; Holopainen, Anu; Aspinen, Samuli; Eskelinen, Matti

doi:10.21873/anticanres.13179

Cited by 6 publications

(5 citation statements)

References 19 publications

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“…Accordingly, we and others have repeatedly shown that compared to the classic open approach, robot-assisted colorectal surgery is associated with attenuated inflammatory and beneficial immune response, which translates into favorable clinical outcomes [58][59][60][61][62][63]. No significant difference in plasma 3-NT with respect to the invasiveness of the procedure observed here is in line with previous observations concerning minilaparotomy cholecystectomy and laparoscopy cholecystectomy [64]. The 3-BT displayed distinct dynamics depending on the presence of serious postoperative complications and surgical site infections.…”

Section: Discussionsupporting

confidence: 91%

Simultaneous LC-MS/MS-Based Quantification of Free 3-Nitro-l-tyrosine, 3-Chloro-l-tyrosine, and 3-Bromo-l-tyrosine in Plasma of Colorectal Cancer Patients during Early Postoperative Period

et al. 2020

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Quantification with satisfactory specificity and sensitivity of free 3-Nitro-l-tyrosine (3-NT), 3-Chloro-l-tyrosine (3-CT), and 3-Bromo-l-tyrosine (3-BT) in biological samples as potential inflammation, oxidative stress, and cancer biomarkers is analytically challenging. We aimed at developing a liquid chromatography–tandem mass spectrometry (LC-MS/MS)-based method for their simultaneous analysis without an extract purification step by solid-phase extraction. Validation of the developed method yielded the following limits of detection (LOD) and quantification (LOQ) for 3-NT, 3-BT, and 3-CT: 0.030, 0.026, 0.030 ng/mL (LODs) and 0.100, 0.096, 0.098 ng/mL (LOQs). Coefficients of variation for all metabolites and tested concentrations were <10% and accuracy was within 95–105%. Method applicability was tested on colorectal cancer patients during the perioperative period. All metabolites were significantly higher in cancer patients than healthy controls. The 3-NT was significantly lower in advanced cancer and 3-BT showed a similar tendency. Dynamics of 3-BT in the early postoperative period were affected by type of surgery and presence of surgical site infections. In conclusion, a sensitive and specific LC-MS/MS method for simultaneous quantification of free 3-NT, 3-BT, and 3-CT in human plasma has been developed.

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Section: Discussionsupporting

confidence: 91%

Simultaneous LC-MS/MS-Based Quantification of Free 3-Nitro-l-tyrosine, 3-Chloro-l-tyrosine, and 3-Bromo-l-tyrosine in Plasma of Colorectal Cancer Patients during Early Postoperative Period

et al. 2020

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“…It is formed as a result of nitration of tyrosine phenolic units in tissues and proteins of the blood, contributing to increased susceptibility of proteins to the action of proteolytic enzymes. In addition, tyrosine nitration leads to the loss of biological functions of these proteins, and as a consequence, to pathological changes [33][34][35]. The high concentrations of total NO, accompanied by unchanged NT concentrations, determined in the serum of patients with mandibular fractures indicate that this molecule does not participate in the oxidative modification of proteins.…”

Section: Discussionmentioning

confidence: 99%

Levels of Biological Markers of Nitric Oxide in Serum of Patients with Mandible Fractures

Woźniak

Ratajczak–Wrona

Borys

et al. 2021

JCM

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Background: Nitric oxide is a small gaseous molecule with significant bioactivity. It has been observed that NO may have a dual role dependent on its production and concentrations in the bone microenvironment. The objective of the study was to assess the concentration of total nitric oxide malonyldialdehyde, nitrotyrosine, and asymmetric dimethylarginine in the serum of patients with mandibular fractures and to understand the relationship between these compounds, in order to expand the knowledge base of the role of nitric oxide and its activity indicators in the process of bone fracture healing. Material and Methods: The study included 20 patients with mandibular fractures who were undergoing inpatient and outpatient treatments and a control group of 15 healthy people. Results were analyzed with respect to the measurement time. Total nitric oxide concentration in the blood serum was determined according to the Griess reaction, while the concentration of malonyldialdehyde, nitrotyrosine, and asymmetric dimethylarginine was estimated using the immunoenzymatic method (i.e., enzyme-linked immunosorbent assay). Results: Before the procedure, as well as on the first day and 2 and 6 weeks after the procedure, higher concentrations of total nitric oxide and lower concentrations of malonyldialdehyde were observed in the blood serum of patients with mandibular fractures compared to the control group. No statistically significant differences were found in nitrotyrosine concentrations in the blood serum of patients throughout the measurement period. However, a significantly higher asymmetric dimethylarginine concentration was observed in the patient serum before the procedure and on the first day of operation as compared with the control group. Analysis of the results observed in patient serum with respect to the number of fractures within the mandible demonstrated the same trend of concentrations for the tested compounds for the entire study group. Conclusions: In summary, our results revealed that the intensity of local processes resulting from mandibular fractures is associated with the concentration of nitric oxide, confirming its significant role, as well as that of its indicators, in the process of bone fracture healing in this patient population.

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“…It is formed as a result of nitration of tyrosine phenolic units in tissues and proteins of the blood, contributing to increased susceptibility of proteins to the action of proteolytic enzymes. In addition, tyrosine nitration leads to the loss of biological functions of these proteins, and as a consequence, to pathological changes [34][35][36]. The high concentrations of total NO, accompanied by unchanged NT concentrations, determined in the serum of patients with mandibular fractures indicate that this molecule does not participate in the oxidative modification of proteins.…”

Section: Discussionmentioning

confidence: 99%

Levels of Biological Markers of Nitric Oxide in Serum of Patients with Mandible Factures

Ratajczak–Wrona

Woźniak

Borys

et al. 2020

Preprint

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Background Nitric oxide is a small gaseous molecule with significant bioactivity. It has been observed that NO may have a dual role dependent on its production and concentrations in the bone microenvironment. The objective of the study was to assess the concentration of total nitric oxide malonyldialdehyde, nitrotyrosine, and asymmetric dimethylarginine in the serum of patients with mandibular fractures and to understand the relationship between these compounds, in order to expand the knowledge base of the role of nitric oxide and its activity indicators in the process of bone fracture healing.Material and methods The study included patients with mandibular fractures who were undergoing inpatient and outpatient treatments. Results were analyzed with respect to the measurement time. Total nitric oxide concentration in the blood serum was determined according to the Griess reaction, while the concentration of malonyldialdehyde, nitrotyrosine, and asymmetric dimethylarginine was estimated using the immunoenzymatic method (i.e. enzyme-linked immunosorbent assay).Results Before procedure as well as on the first day and 2 and 6 weeks after the procedure, higher concentrations of total nitric oxide and lower concentrations of malonyldialdehyde were observed in the blood serum of patients with mandibular fractures compared to the control group. No statistically significant differences were found in nitrotyrosine concentrations in the blood serum of patients throughout the measurement period. However, a significantly higher asymmetric dimethylarginine concentration was observed in the patient serum before the procedure and on the first day of operation as compared with the control group. Analysis of the results observed in patient serum with respect to the number of fractures within the mandible demonstrated the same trend of concentrations for the tested compounds for the entire study group.Conclusions In summary, our results revealed that the intensity of local processes resulting from mandibular fractures is associated with the concentration of nitric oxide, confirming its significant role, as well as that of its indicators, in the process of bone fracture healing in this patient population.

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Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer Patients

Cited by 6 publications

References 19 publications

Simultaneous LC-MS/MS-Based Quantification of Free 3-Nitro-l-tyrosine, 3-Chloro-l-tyrosine, and 3-Bromo-l-tyrosine in Plasma of Colorectal Cancer Patients during Early Postoperative Period

Simultaneous LC-MS/MS-Based Quantification of Free 3-Nitro-l-tyrosine, 3-Chloro-l-tyrosine, and 3-Bromo-l-tyrosine in Plasma of Colorectal Cancer Patients during Early Postoperative Period

Levels of Biological Markers of Nitric Oxide in Serum of Patients with Mandible Fractures

Levels of Biological Markers of Nitric Oxide in Serum of Patients with Mandible Factures

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