Plasma Antihemophilic Activity Following Total Body Irradiation.

The Complete Spleen

“…FACTOR VIII (Antihemophilic Factor) The search for the exact cell site( s) of factor VIII synthesis began in earnest in the early 1950s (Penick et ai., 1970;Dodds and Hoyer, 1974a;Bloom, 1979), and remains controversial today (Dodds, 1997). Investigators at the University of North Carolina utilized normal dogs exposed to total body irradiation, chloroform, and carbon tetrachloride (Graham et ai., 1951;Penick et al, 1951), and hemophilic dogs (Grahamet ai., 1951), to show that factor VIII levels were not significantly affected by marrow failure and hepatotoxins. By contrast, platelet counts were severely depressed and prothrombin utilization was impaired.…”

Generation of Factor VIII Coagulant Activity by Isolated, Perfused Neonatal Pig Livers and Adult Rat Livers

“…The reticuloendothelial system has been implicated because ethionine poisoning depresses VIII : C (Pool & Spaet, 1954) and typhoid vaccination raises this factor (Egeberg, 1962). However, whole body irradiation does not lower plasmatic VIII:C (Penick et al, 1951), nor does administration of methyl palmitate which sharply reduced clearance of carbon particles from the circulation (Gaynor & Spaet, 1966). The lung has been proposed as a site of extrahepatic synthesis of VIII:C (Veltkamp et al, 1974) as has the bone marrow (Nilihn, 1962).…”