The Role of the Splenic Macrophage in Antihemophilic Factor (Factor VIII) Synthesis

Tissue Factor Activity in Lymphocyte Cultures from Normal Individuals and Patients with Hemophilia A

“…Similar results from human fibroblasts have been presented by Green, Ryan, Malandruccolo, and Nadler (55). The failure of other investigators to find procoagulant activity in cultures of either rabbit spleen lymphocytes (52) or canine lymphocytes (56,57) may merely reflect either species differences or methodological variations. We found almost no procoagulant activity released into the supernatant media of the cultures and therefore used lysates of cells exclusively.…”

Antihemophilic Factor Antigen. LOCALIZATION IN ENDOTHELIAL CELLS BY IMMUNOFLUORESCENT MICROSCOPY

“…(1-5), spleen (2,3,6,7), kidneys (8), lymphocytes (9), and macrophages (10) have been reported. These previous investigations have reported AHF synthesis and/or release from perfused organs (2,3,7), specific cell types in tissue culture (9,10), and in AHF-deficient animals given organ transplants (4,5).…”

“…These previous investigations have reported AHF synthesis and/or release from perfused organs (2,3,7), specific cell types in tissue culture (9,10), and in AHF-deficient animals given organ transplants (4,5). Such studies are limited by the lability of AHF procoagulant activity, however, and by the difficulty in distinguishing changes in AHF activity from nonspecific shortening of clottingtime assays by other procoagulant materials.…”

Characterization of procoagulant activity produced by cultures of human monocytes and lymphocytes separated in colloidal silica-polvinylpyrrolidone gradients

“…In this respect synthesis of FVIIIRAG has been localized in human umbilical cord endothelial cells (Jaffe et al 1973, Shearn et al 1977 and in megakaryocytes (Nachman & Jaffe 1977). In many instances demonstration of FVIlLC synthesis is complicated by the presence of tissue Correspondence: Dr J.C.Giddings, Department of Haernatology, Welsh National School of Medicine, Heath Park, Cardiff. thromboplastin or other non-specific coagulants and for this reason the activity generated in cultures of mononuclear cells from the spleen (Ponn et al 1971), peripheral blood leucocytes (Zacharski et al 1968) and human skin fibroblasts (Zacharski et al 1969) was probably not specifically FVIIIC (Kociba et al 1972, Rickles et a/. 1973.…”