Plasma and urinary free 3-nitrotyrosine following cardiac angiography procedures with non-ionic radiocontrast media

Abstract

The present study provides indirect evidence that RCM administration in humans is associated with an increased production of 3-NT. Further studies are needed to ascertain whether oxygen- and nitrogen-derived radical species play a major role in the pathogenesis of RCM-associated nephrotoxicity in the clinical setting.

Determination of 3-nitrotyrosine in human urine at the basal state by gas chromatography–tandem mass spectrometry and evaluation of the excretion after oral intake

“…Moreover, by means of HPLC with UV absorbance detection, similar to that used here (i.e. with a LOD of approximately 100 nM for 3-nitrotyrosine), 3-nitrotyrosine was measured in urine of patients administered with radiocontrast media at approximately 5.5 M or 11.3 mol/mmol creatinine [16]. These values are more than 1000 times higher than those measured by GC-tandem MS in the present study in healthy humans.…”

“…Various groups reported that 3-nitrotyrosine is excreted in the urine of healthy humans [13][14][15][16]. Reported excretion rates and urinary concentrations of 3-nitrotyrosine vary between 60 nmol/day [15] and 248 nmol/day [13], i.e.…”

“…Reported excretion rates and urinary concentrations of 3-nitrotyrosine vary between 60 nmol/day [15] and 248 nmol/day [13], i.e. 4-fold, and between 36 nM [14] and 5 M [16], i.e. 139-fold, respectively, strongly suggesting serious methodological problems in the quantification of urinary 3-nitrotyrosine, too.…”

Contrast induced nephropathy - cardiologist perspective

“…The malondialdehydeto-creatinine ratio increased following contrast infusion, suggesting a link between contrast infusion and free radical generation. In a study investigating the effects of cardiac catheterization on the generation of reactive oxygen species, Fiaccadori and colleagues 30 measured urine and plasma levels of 3-nitrotyrosine(a marker of peroxynitrite generation from superoxide).Urinary 3-nitrotyrosine peaked after catheterization, but plasma levels remained elevated for up to 72 hours. However, in 193 lowrisk patients with normal renal function studied prospectively, there was no significant difference in the baseline serum levels of antioxidant compounds between those who did or did not develop renal failure after cardiac catheterization.…”

3-Nitrotyrosine attenuates respiratory syncytial virus infection in human bronchial epithelial cell line

“…This nitrative stress is evidenced by the presence of nitrated protein tyrosine in the tissues (9,43) and by increased free 3-nitrotyrosine (NO2Tyr) in biological fluids (13,23). Experimental evidence has indicated that nitration of tyrosine residues in proteins may alter protein function and protein turnover and modify signal transduction processes (24).…”