Determination of 3-nitrotyrosine in human urine at the basal state by gas chromatography–tandem mass spectrometry and evaluation of the excretion after oral intake

Tsikas, Dimitrios; Mitschke, Anja; Suchy, Maria-Theresia; Gutzki, Frank‐Mathias; Stichtenoth, Dirk O.

doi:10.1016/j.jchromb.2005.03.039

Cited by 49 publications

(45 citation statements)

References 22 publications

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“…The levels of the measured concentrations of the markers correspond to ranges identified in previously published data, which were measured on the different groups of healthy, non-pregnant subjects (11,12,16,25,26). The concentrations of the markers vary between studies.…”

Section: Discussionsupporting

confidence: 68%

“…The concentrations of the markers vary between studies. These studies in healthy non-pregnant women reported that the urinary HEL levels were between 1.58±0.23 and 2.3±1.2 µmol/mol Cr (11,12,16), PRL levels were 21.6±10.6 µmol/mol Cr (11), DiY levels were between 8.8±0.6 and 10.1±0.4 µmol/mol Cr (16,25) and NY levels were between 0.46±0.49 and 1.4±0.4 µmol/mol Cr (16,26).…”

Section: Discussionmentioning

confidence: 99%

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Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Rejc

Kato

Karas-Kuzelicki

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Pregnancy is a physiological state accompanied by excessive levels of oxidative stress (OS), due to the increased demand and utilisation of oxygen. There is increasing evidence that maternally augmented OS exerts an adverse effect on pregnancy outcome. The aim of the present prospective study was to determine the association between the urinary concentration of relatively novel OS markers measured in the second trimester of pregnancy and the infant characteristics at birth. The maternal levels of urinary hexanoyl-lysine (HEL), propanoyl-lysine (PRL), dityrosine (DiY) and 3-nitrotyrosine (NY) were evaluated in generally healthy pregnant subjects to determine their association with birth weight, gestation at delivery and Apgar score. The observed levels of the markers were in agreement with those measured in healthy non-pregnant subjects in a previous study. A positive correlation was detected between HEL and PRL, as well as between HEL and DiY. Although the absence of a correlation between NY and the other markers has been previously noted in a non-pregnant population, a positive correlation in the pair PRL-NY (r=0.367; P<0.001) was observed in the present study. Maternal cigarette smoking was associated with increased urinary PRL levels (P=0.034). The most notable observation in the present study was that high levels of PRL and NY were associated with low Apgar scores at 1 and 5 min after birth (OR, 1.098 and 2.084 for PRL and NY, respectively; P<0.05). However, poor predictive accuracy was shown. For NY, the following results were obtained: Area under the curve (AUC), 0.818; sensitivity, 100%; specificity, 57%; positive predictive value (PPV), 11.54%; and negative predictive value (NPV), 100%.For PLR the values were as follows: AUC, 0.802; sensitivity, 100%; specificity, 62.6%; PPV, 13.05%; and NPV, 100%. DiY was negatively associated with preterm birth risk (OR=0.703; P=0.028). In conclusion, the results of the present study indicated the presence of OS in the second trimester of pregnancy, which was detected with damage to lipids and proteins and associated with an adverse Apgar score; however, the selected urinary markers exhibited poor positive predictive efficacy.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Rejc

Kato

Karas-Kuzelicki

et al. 2016

Experimental and Therapeutic Medicine

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“…Detection of reactive nitrogen species in EPCs 3-Nitrotyrosine assay. Peroxynitrite reacts quickly with a variety of different biomolecules to produce 3-nitrotyrosine, which therefore is a good biomarker for reactive nitrogen species (24). Human EPCs were treated with glucose (30 mmol/l, 24 h), and then cell lysates were prepared on ice using cell lysis buffer (1 mol/l Tris-HCl [pH 7.6], 0.5 mol/l EDTA, and 1.25 mol/l dithiothreitol).…”

mentioning

confidence: 99%

“…Human EPCs were treated with glucose (30 mmol/l, 24 h), and then cell lysates were prepared on ice using cell lysis buffer (1 mol/l Tris-HCl [pH 7.6], 0.5 mol/l EDTA, and 1.25 mol/l dithiothreitol). 3-Nitrotyrosine from cell lysates was then isolated by HPLC and subjected to a sensitive gas chromatography-tandem mass spectrometry method, as recently described by us (24), in the electron capture negative-ion chemical ionization mode on a triple-stage quadrupole mass spectrometer model ThermoQuest TSQ 7,000 (Finnigan MAT, San Jose, CA) directly interfaced with a Trace 2000 series gas chromatograph equipped with an autosampler AS 2000 (CE Instruments, Austin, TX) (24). Measurements of biopterin content in EPCs.…”

mentioning

confidence: 99%

Endothelial Nitric Oxide Synthase Uncoupling Impairs Endothelial Progenitor Cell Mobilization and Function in Diabetes

Thum¹,

Fraccarollo²,

Schultheiß³

et al. 2007

Diabetes

Self Cite

362

259

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“…Presumably, nitrosated amino acids are less efficient substrates in metabolic and protein synthesis pathways, which may lead to increases of nitrosated and oxidized aromatic compounds in the cytoplasm, specific tissues, circulation and urinary excretion. From the analytical perspective, the detection of nitrosated small molecules can be used to qualify systemic as well as organ-and tissue-specific NO-induced toxicity [178][179][180].…”

Section: Aromatic Nitrosation 3-nitrotyrosine and N-nitrosotryptophamentioning

confidence: 99%

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Boudko

2007

Journal of Chromatography B

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This review briefly summarizes recent progress in fundamental understanding and analytical profiling of the L-arginine/nitric oxide (NO) pathway. It focuses on key analytical references of NO actions and on the experimental acquisition of these references in vivo, with capillary electrophoresis (CE) and high-performance capillary electrophoresis (HPCE) comprising one of the most flexible and technologically promising analytical platform for comprehensive high-resolution profiling of NO-related metabolites. Second aim of this review is to express demands and bridge efforts of experimental biologists, medical professionals and chemical analysis-oriented scientists who strive to understand evolution and physiological roles of NO and to develop analytical methods for use in biology and medicine.

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Determination of 3-nitrotyrosine in human urine at the basal state by gas chromatography–tandem mass spectrometry and evaluation of the excretion after oral intake

Cited by 49 publications

References 22 publications

Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Endothelial Nitric Oxide Synthase Uncoupling Impairs Endothelial Progenitor Cell Mobilization and Function in Diabetes

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

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