Plasma and tissue concentrations of α-tocopherol during vitamin E depletion in sheep

Fry, JM; Smith, G. M.; McGrath, MC; Speijers, EJ; Allen, J. G.

doi:10.1079/bjn19930024

Cited by 23 publications

(26 citation statements)

References 20 publications

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“…These results agree with those reported by Asghar et al (1991) for pigs 5 to 14 wk of age and with those reported by Fry et al (1993) for sheep. Values of CK activity in plasma in the present experiment ranged from 973 to 2,593 IU, except at 14 d of age.…”

Section: Discussionsupporting

confidence: 96%

Efficacy of Dietary and Injected Vitamin E for Poults

Soto-Salanova

Sell

1996

Poultry Science

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An experiment was conducted to compare the efficacy of two dietary sources and an injectable form of vitamin E (VE) to improve the VE status of poults. Six of the treatments consisted of a factorial arrangement of three concentrations and two sources of dietary VE. Turkeys in these treatments received 12, 80, or 150 IU of either dl-alpha-tocopheryl acetate or d-alpha-tocopherol (d-alpha-TOC)/kg of diet. The seventh treatment consisted of a single subcutaneous injection of d-alpha-TOC at 1 d of age. Poults in this treatment were subcutaneously injected in the dorsal area of the neck with 25 IU of d-alpha-TOC, this amount being approximately equivalent to the amount poults would consume if their diet was supplemented with 150 IU of VE/kg during their 1st wk of life. Concentration, source, or route of VE administration did not affect growth parameters, plasma creatine kinase, plasma triglycerides, or liver lipid peroxidation as measured by the thiobarbituric acid reactive substances assay (TBARS). Plasma, red blood cells (RBC), and liver alpha-TOC decreased from hatching to 14 d of age in poults fed either source of VE. The use of 80 or 150 IU of dietary VE (either source) reduced (P < 0.05) the extent of depletion of alpha-TOC at all ages and also reduced the susceptibility of RBC to hemolysis. There was no effect of source of dietary VE on concentration of alpha-TOC in plasma, RBC, or liver, or on RBC hemolysis. Subcutaneous injection of 25 IU of d-alpha-TOC at Day 1 increased (P < 0.05) alpha-TOC concentration until 7 d of age. Also, d-alpha-TOC injection reduced (P < 0.05) RBC susceptibility to hemolysis through 21 d of age. Data showed that one single subcutaneous injection of 25 IU of d-alpha-TOC at 1 d of age was as effective as 80 IU or more of dietary VE through 21 d to improve the alpha-TOC status of poults.

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Section: Discussionsupporting

confidence: 96%

Efficacy of Dietary and Injected Vitamin E for Poults

Soto-Salanova

Sell

1996

Poultry Science

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“…This threshold concentration can be easily reached by supplementing lambs with 275IU -tocopherol acetate/kg of feed (daily requirement being 440mg-tocopherol acetate/sheep.day) for a period of 3 to 4 weeks. Because the depletion of vitamin E in the muscle can occur rapidly (Fry et al, 1993), it is important that the supply of -tocopherol be continual for this period, up until…”

Section: Discussionmentioning

confidence: 99%

“…Lambs were sourced in summer from a commercial farm where they grazed dry pasture supplemented with lupin grain at the rate of 600g/lamb/day, conditions in which tissue vitamin E levels are rapidly depleted (Fry, Smith, McGrath, Speijers & Allen, 1993). Initial plasma measurements, confirmed that all animals entering the experiment were deficient or close to deficient in vitamin E (< 0.5mg/L; (Menzies, Langs, Boermans, Martin & McNally, 2004)).…”

Section: Lambs and Dietary Treatmentsmentioning

confidence: 99%

Short term supplementation rates to optimise vitamin E concentration for retail colour stability of Australian lamb meat

et al. 2016

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The relationship between vitamin E supplementation rate and colour stability was investigated using 70 mixed sex 6-8 month old crossbred lambs. An initial group of 10 were slaughtered, while the remainder were fed a pellet ration containing either 30, 150, 275 or 400IU Vitamin E/kg ration or on green pasture for 56 days. After slaughter, carcases were halved; one side packed fresh (5 days) and the other in CO 2 (21 days), both at 2 o C. Five muscles were set for retail display for 96 hours. The oxy/metmyoglobin ratio was measured every 12 hours. Colour stability increased with increasing muscle vitamin E until an apparent maximum effect for vitamin E concentration (3.5-4.0mg -tocopherol/kg tissue) was reached beyond which no further response was evident. This was reached within 3-4 weeks (275IU treatment), and meat from these lambs should reach 60 hours retail display with a satisfactory surface colour. This effect was most apparent in aerobic muscle types and meat aged post slaughter.

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“…As a fat-soluble vitamin, a proportion of vitamin E is stored in adipose tissue; together with liver and muscle, adipose tissue may account for more than 90% of vitamin E in the body (Debier and Larondelle, 2005). Tissue uptake (Toutain et al, 1995) and depletion (Fry et al, 1993;Hidiroglou et al, 2003) studies have shown that adipose tissue acquires vitamin E more avidly and is depleted at a similar rate to muscle. In the current study and those of Capper et al (2005) and Merrell (1999) the concentrate feed offered to the sheep was intended to result in some mobilisation of adipose tissue during late pregnancy.…”

Section: Discussionmentioning

confidence: 99%

The effect of late pregnancy supplementation of ewes with vitamin E on lamb vigour

Rooke¹,

Matheson²,

Ison³

et al. 2009

Animal

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The experiment measured lamb responses to supplementation of the pregnant ewe diet with vitamin E above requirement. Crossbred ewes were mated with either Suffolk or Texel rams. Twin-bearing ewes were randomly allocated (approximately 21 months of age at allocation) to one of four treatment groups (20 ewes per group, 10 mated with Suffolk and 10 with Texel rams). Treatments imposed were 50, 100, 150 or 250 IU supplementary vitamin E per ewe per day to give a four treatment by two sire-type factorial experimental design. Ewes were fed concentrates to meet energy requirements for stage of pregnancy and hay ad libitum. Diets were introduced approximately 6 weeks before lambing. Blood samples were obtained prior to introduction of diets, 17 days after introduction of diets and within 24 h of lambing from a subset of eight ewes per treatment (32 total). Colostrum samples were obtained from 10 ewes per treatment, 12 h after birth of the first lamb. All births were observed and a lamb vigour score was assigned to each lamb 5 min after birth. At 1 and 12 h after birth, rectal temperature, and at 12 h after birth, sex, crown-rump length and BW of each lamb were recorded. Mean ewe plasma a-tocopherol concentration prior to introduction of the diets was 1.5 mg/ml (s.e.m. 0.09) and did not differ between groups. There were positive linear ( P , 0.001) effects of dietary vitamin E on plasma (17 days after introduction of diets) and colostrum (12 h after birth) a-tocopherol concentrations. Lamb vigour scores were superior ( P , 0.001) for lambs sired by Texel rather than Suffolk rams but there were no differences as a result of vitamin E supplementation. Lamb mortality was low and unrelated to either sire or supplementary vitamin E. Lamb birth and weaning weights were also unaffected by vitamin E supplementation. Supplementing the ewe with vitamin E therefore had no effect on any lamb measurements.

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Plasma and tissue concentrations of α-tocopherol during vitamin E depletion in sheep

Cited by 23 publications

References 20 publications

Efficacy of Dietary and Injected Vitamin E for Poults

Efficacy of Dietary and Injected Vitamin E for Poults

Short term supplementation rates to optimise vitamin E concentration for retail colour stability of Australian lamb meat

The effect of late pregnancy supplementation of ewes with vitamin E on lamb vigour

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