Plasma acylation stimulating protein, adipsin and lipids in non‐obese and obese populations

Maslowska, Magdalena; Vu, Hung V.; Phélis, Steve; Ad, Sniderman; Bm, Rhode; Blank, David; Cianflone, Katherine

doi:10.1046/j.1365-2362.1999.00514.x

Cited by 132 publications

(110 citation statements)

References 34 publications

(48 reference statements)

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“…However, in studies involving humans, adipsin was found to be higher with increased body mass. 22,23 The upregulation of adipsin gene expression during adipogenesis in this study correlates well with similar observation reported by other research groups. 15,24 PPARg is the most adipose specific of the PPAR subfamily of nuclear hormone receptors, and exists as two isoforms: PPARg 1 and PPARg 2 .…”

Section: Discussionsupporting

confidence: 92%

Cord lining progenitor cells: potential in vitro adipogenesis model

et al. 2010

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Objective: To investigate the effect of glucose and insulin concentrations on differentiation of umbilical cord lining progenitor cells to adipocyte-like cells (ALCs). Methods: Cord lining mesenchymal cells (CLMCs) were isolated from the explant of human umbilical cord amniotic membrane. CLMCs were subjected to differentiation under various culture conditions for 20 days. Lipid droplets were confirmed with Oil Red O staining. Gene expressions of adipsin and peroxisome proliferator-activated receptor gamma (PPARg) were analyzed using reverse transcription-PCR. Leptin and adiponectin secretions were detected using enzyme-linked immunosorbent assay kit. Results: CLMCs became irregular, cuboidal-shaped cells that resemble adipocytes, and Oil Red O staining showed the presence of lipid droplets. The gene expressions of PPARg and adipsin were upregulated. Leptin and adiponectin secretions by naive CLMCs were below the limits of detection. Matured ALCs cultured in low-glucose medium significantly secreted leptin and adiponectin, whereas those in high-glucose medium significantly secreted only leptin. Insulin concentration affects leptin but not adiponectin secretion. Conclusions: Under different culture conditions, CLMCs can differentiate into ALCs that resemble adipocytes in either normalweight or obese individuals. Hence, these ALCs have the potential to be used as an in vitro model to study adipogenesis and obesity, and possibly as a drug discovery model for metabolic disorders.

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Section: Discussionsupporting

confidence: 92%

Cord lining progenitor cells: potential in vitro adipogenesis model

et al. 2010

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“…In this sense, a previous study (Maslowska et al, 1999) explains these results through the ASP activity rising the glucose transport additively and independently of insulin mechanisms, and stimulating the diacylglycerol acyltransferase enzyme, involved in triacylglycerol synthesis pathway. In agreement with insulin concentrations, and in contrast to our results, positive correlation with circulating C3 has been described (Yudkin, 2000;Halkes et al, 2001;Ylitalo et al, 2001), highlighting its possible role as a predictor of risk to develop type 2 diabetes and related metabolic disorders (Engstrom et al, 2005b).…”

Section: Discussionmentioning

confidence: 77%

“…As ASP, a product derived from C3, rises the efficiency of synthesis of triacylglycerols in adipocytes, stimulating glucose absorption, activating the diacylglycerol acyltransferase (DGAT) and inhibiting the hormone-sensitive lipase (Sniderman et al, 2000) and high concentrations of C3 are able to increase circulating triacylglycerols (Maslowska et al, 1999), the C3/ASP system has been hypothesized as a modulator of the fatty acid metabolism in adipose tissue. On the other hand, C3 knockout mice do not respond to a high fat diet with ethanol, tending to reduce liver steatosis (Bykov et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Selenium intake reduces serum C3, an early marker of metabolic syndrome manifestations, in healthy young adults

et al. 2008

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Objectives: To evaluate the associations between serum complement factor 3 (C3) and several anthropometrical, biochemical and lifestyle features in healthy young adults, emphasizing on the putative effect of selenium intake on C3 concentrations. Methods: This study enrolled 100 healthy young adults aged 18-34 years. Anthropometric and blood pressure measurements and lifestyle features were analyzed. Fasting blood samples were collected for the measurement of glucose, total cholesterol, HDL-cholesterol, LDL-cholesterol, triacylglycerols and C3 concentrations. Nail samples were collected for the analysis of selenium concentrations. Results: Values of BMI (P ¼ 0.034), sum of skinfold thicknesses (STs) (P ¼ 0.021), body fat mass (BFM) (P ¼ 0.023), percentage of overweight subjects (P ¼ 0.007), serum triacylglycerols (P ¼ 0.012) and nail selenium (P ¼ 0.001) were significantly different between subjects above and below the median of serum C3 concentrations. The following correlations with serum C3 were identified tricipital ST (P ¼ 0.033), sum of STs (P ¼ 0.012), BMI (P ¼ 0.008), BFM (P ¼ 0.018), waist-to-height ratio (P ¼ 0.016), serum glucose (P ¼ 0.045), serum triacylglycerols (P ¼ 0.001) and nail selenium (P ¼ 0.006). Circulating C3 showed a positive association with several adiposity markers such as BMI (P ¼ 0.001), waist circumference (P ¼ 0.006), waist-to-height ratio (P ¼ 0.002), BFM (P ¼ 0.025), as well as serum glucose (P ¼ 0.027) and triacylglycerols (Po0.001), whereas nail selenium was a statistically significant negative predictor of C3 concentrations (P ¼ 0.018). Conclusions: C3 seems to be related with selenium status and several anthropometrical and biochemical measurements linked to metabolic syndrome in apparently healthy young adults. These findings suggest a possible role for selenium intake in the modulation of C3, whose assessment may be an early marker of metabolic syndrome manifestations.

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“…Interestingly, however, several studies have shown that ASP levels are high in obese subjects, possibly as a direct consequence of increased fat mass. 30,29,11,12 It was recently hypothesized that these high ASP levels represent an ASP-resistant state, whereas low ASP levels indicate increased sensitivity of adipose tissue towards ASP. 14 This hypothesis is supported by in vitro data.…”

Section: Discussionmentioning

confidence: 99%

“…10,29 In one subject, fasting ASP could not be determined post-training due to technical problems. For determination of FFA, glycerol, triglycerides and glucose, blood was collected in tubes containing 30 ml of 0.2 M EDTA.…”

Section: Asp and Endurance Training P Schrauwen Et Almentioning

confidence: 99%

Acylation-stimulating protein: effect of acute exercise and endurance training

et al. 2005

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INTRODUCTION: Acylation-stimulating protein (ASP) is an adipocyte-derived protein that contributes to fatty acid clearance. Regular exercise training improves fatty acid handling. OBJECTIVE: To examine the effect of acute exercise and short-term endurance training on ASP levels. SUBJECTS: Eight untrained men (age: 23.573.4 y; maximal power output (W max ): 3.770.6 W/kg body weight). DESIGN: Subjects were trained for 2 weeks. Before and after training, blood was sampled during a 3-h exercise test, and insulin sensitivity was assessed by an insulin tolerance test. RESULTS: Before training, ASP levels decreased during exercise (from 17.972.9 to 15.573.7 nmol/l at t ¼ 0 vs 180, Po0.05). Endurance training decreased fasting ASP levels significantly (17.972.9 vs 13.472.3 nmol/l pre-and post-training, Po0.001). Interestingly, after 2 weeks of endurance training, ASP levels tended to increase during exercise (from 13.472.3 to 17.274.5 nmol/l at t ¼ 0 vs 180, P ¼ 0.09). Baseline ASP levels correlated negatively with insulin sensitivity both before (r ¼ À0.86, Po0.01) and after training (r ¼ À0.82, Po0.05). CONCLUSION: Short-term endurance training reduces baseline ASP levels. These data fit with the hypothesis that reduced ASP levels indicate improved ASP sensitivity.

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Plasma acylation stimulating protein, adipsin and lipids in non‐obese and obese populations

Abstract: The present data suggest involvement of the ASP/adipsin pathway in the pathogenesis of obesity.

Cited by 132 publications

References 34 publications

Cord lining progenitor cells: potential in vitro adipogenesis model

Cord lining progenitor cells: potential in vitro adipogenesis model

Selenium intake reduces serum C3, an early marker of metabolic syndrome manifestations, in healthy young adults

Acylation-stimulating protein: effect of acute exercise and endurance training

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