Plasma

Identification and characterization of podocalyxin--the major sialoprotein of the renal glomerular epithelial cell.

“…This conclusion relies on the facts that (a) it is the only band in SDS gels of glomerular extracts that is stained intensely blue by the cationic dyes, alcian blue, and Stains All (16,17); (b) it is virtually the only band that binds ['']WGA in nitrocellulose transfers, and WGA binding is prevented by pretreatment with neuraminidase, indicating that sialic acid is responsible for the binding (28); and (c) it is the most prominent band in fluorograms of glomerular lysates after selective labeling of sialic acid residues by the periodate-['H]borohydride method (30). Moreover, it contains 4 .5% sialic acid by the Warren assay, being comparable to fetuin in this respect (37). The high efficiency of extraction of sialic acid from glomeruli (~85-95%) obtained under the conditions used indicate that we are dealing with a major glomerular sialoprotein rather than a minor component selectively enriched by the extraction procedure.…”

Release Kinetics from Bio-Polymeric Nanoparticles Encapsulating Protein Synthesis Inhibitor- Cycloheximide, for Possible Therapeutic Applications

“…Gelatin, a naturally occurring biopolymer has definite advantages over synthetic polymers. Also, its biodegradability and biocompatibility are well documented in literature [5]. Release of BSA from gelatin microspheres have been reported by Goracinova et al [6].…”

“…Gelatin microspheres have been used as adjuvants, to enhance both the humoral and cellular responses to antigen [7]. Gelatin has been used as efficient carrier systems for drugs and proteins [5,6]. Gelatin nanoparticles in the 20-100nm range, prepared in the reverse phase have been reported recently [8], but reverse phase preparations are known to be limited by percent yield.…”