Biological Significance of Sialic Acids

Reutter, Werner; Köttgen, E.; Bauer, Christian; Gerok, W.

doi:10.1007/978-3-7091-8680-0_10

Cited by 87 publications

(27 citation statements)

References 378 publications

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“…Other studies similarly have shown that immature cells possess many sialic acid residues, whereas aging reduces their number (38). WGA binding to cells migrating to repair an epithelial injury was also shown to be much greater than binding to stationary cells (7).…”

Section: Discussionmentioning

confidence: 77%

Differential adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells in primary culture.

Plotkowski

Chevillard²,

Pierrot³

et al. 1991

J. Clin. Invest.

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Human nasal polyps in outgrowth culture were used to study the Pseudomonas aeruginosa adhesion to respiratory cells. By scanning electron microscopy, P. aeruginosa were seen associated with ciliated cells, but by transmission electron microscopy, bacteria were never seen at the interciliary spaces or attached along cilia, but were identified trapped at the extremities of cilia, usually as bacterial aggregates. A fibronectin-containing fibrillar material was seen associated with aggregated bacteria. By time-lapse video microscopy, bacteria were seen to aggregate in the culture medium following their addition to the culture wells. Progressively, these aggregates were trapped by cilia or attached to migrating cells of a lower cell layer that protruded beneath the upper layer cells, at the outgrowth periphery. P. aeruginosa adhesion to these lower cell layer migrating cells was significantly higher than to ciliated or nonciliated cells of the upper cell layer. Migrating cells were intensely labeled by the complexes Con A and arachis hypogea agglutinin (PNA)-FITC, in contrast to the other cells. The percentage of PNA-labeled cells with attached bacteria was significantly higher than that without bacteria. These results suggest that changes of cell surface glycoconjugates related with cell migration may favor P. aeruginosa adhesion to respiratory cells. (J. Clin.

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Section: Discussionmentioning

confidence: 77%

Differential adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells in primary culture.

Plotkowski

Chevillard²,

Pierrot³

et al. 1991

J. Clin. Invest.

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“…Consequently, they play important roles in the regulation of cell proliferation, cell adhesion, cancer metastasis, tissue differentiation, and apoptosis (2,3). In particular, sialylation of sugar chains has been suggested to be a very important process during development, cancer evolution, and progression (4), and sialic acid is often responsible for tumor-associated antigenicity.…”

mentioning

confidence: 99%

Synthesis of Disialyl Lewis a (Lea) Structure in Colon Cancer Cell Lines by a Sialyltransferase, ST6GalNAc VI, Responsible for the Synthesis of α-Series Gangliosides

Tsuchida

Okajima

Ando

et al. 2003

Journal of Biological Chemistry

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“…Sialic acid has been shown to play a role in cell adhesion (6,15,19,20,28). Higher adhesiveness of amelanotic and BrdU-treated melanotic cells could be explained by their lower sialic acid levels, as with the case of lectin-resistant cells (2,25).…”

Section: Discussionmentioning

confidence: 99%

“…From comparison of spontaneous phenotypic variations, the correlation of sialic acid level with tyrosinase activity was confirmed, while there was only a slight correlation with adhesiveness. It is thus suggested that sialylation/desialylation, being reflected as variations in cellular sialic acid content, is implicated in melanoma cell differentiation in terms of tyrosinase expression.Sialic acid has been shown to play important roles in a variety of biological processes including cell adhesion, cellular recognition and regulation of glycoconjugates' functions (6,15,19,20,28). In melanoma cells, involvement of sialic acid residues has been well documented in determining metastatic potential (13).…”

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confidence: 99%

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Cellular sialic acid level and phenotypic expression in B16 melanoma cells: Comparison of spontaneous variations and bromodeoxyuridine- and theophylline-induced changes.

Kinoshita

Sato

Takeuchi

1989

Cell Struct. Funct.

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ABSTRACT. The relation of the total cellular content of sialic acid to phenotypic expression of B16 mouse melanoma cells was examined by using phenotype-modifying reagents and more than 10 cloned cell lines with spontaneous phenotypic variations. The sialic acid content changed in a growth phase-dependent manner with a peak in the early log phase of growth. This peak completely disappeared when cells were treated with 5-bromodeoxyuridine (BrdU), suggesting its relation to quasi-normal phenotypes of the treated cells. BrdU treatment also reduced the cellular sialic acid content itself and resulted in the suppression of the activity of tyrosinase, the key enzyme for melanogenesis, and a considerable increase in cell-to-substratum adhesiveness. Treatment with theophylline, in contrast, markedly elevated the sialic acid content, which was accompanied by dramatic increments in tyrosinase activity and pigmentation as well as a slight increase in adhesiveness. The results show a correlation of sialic acid level with tyrosinase expression but not with cell adhesion. From comparison of spontaneous phenotypic variations, the correlation of sialic acid level with tyrosinase activity was confirmed, while there was only a slight correlation with adhesiveness. It is thus suggested that sialylation/desialylation, being reflected as variations in cellular sialic acid content, is implicated in melanoma cell differentiation in terms of tyrosinase expression.Sialic acid has been shown to play important roles in a variety of biological processes including cell adhesion, cellular recognition and regulation of glycoconjugates' functions (6,15,19,20,28). In melanoma cells, involvement of sialic acid residues has been well documented in determining metastatic potential (13). A lectin (WGA)-resistant phenotype and the related alteration of adhesive properties have also been shown to be due to a decrease in cell surface sialic acid content (2, 25). However, little has been known of the functional significance of sialic acid relating to other aspects of melanoma cell phenotypes.Treatment of mouse melanoma cells with 5-bromodeoxyuridine (BrdU) (18, 23) or retinoic acid (1) has been shown to result in changes in cellular sialic acid content.

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Biological Significance of Sialic Acids

Cited by 87 publications

References 378 publications

Differential adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells in primary culture.

Differential adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells in primary culture.

Synthesis of Disialyl Lewis a (Lea) Structure in Colon Cancer Cell Lines by a Sialyltransferase, ST6GalNAc VI, Responsible for the Synthesis of α-Series Gangliosides

Cellular sialic acid level and phenotypic expression in B16 melanoma cells: Comparison of spontaneous variations and bromodeoxyuridine- and theophylline-induced changes.

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