Synthesis of Disialyl Lewis a (Lea) Structure in Colon Cancer Cell Lines by a Sialyltransferase, ST6GalNAc VI, Responsible for the Synthesis of α-Series Gangliosides

Tsuchida, Akiko; Okajima, Tetsuya; Ando, Takayuki; Ishida, Hideyuki; Yoshida, Aruto; Nakamura, Yoko; Kannagi, Reiji; Kiso, Makoto; Furukawa, Koichi

doi:10.1074/jbc.m211034200

Cited by 89 publications

(69 citation statements)

References 23 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some of the data were essentially the same as reported in [21] and [23], but they were confirmed in this study using the identical conditions. Both enzymes showed activity toward fetuin, MSGG and sialyl Lc4 as well as GM1b.…”

Section: Hst6galnac III and Vi Synthesize Dsggsupporting

confidence: 77%

“…MSGG and DSGG were eluted from the DEAE-Sephadex by step-wise elution with sodium acetate (0.2, 0.25, 0.3, 0.45, 0.6 and 0.8 M) in a solvent system of chloroform/methanol/sodium acetate (30:60:8, by vol.). GM1b, sialyl Lc4, and sialyl nLc4 (see Table 2) were prepared as described previously [21].…”

Section: Flow Cytometrymentioning

confidence: 99%

“…ST6GalNAc-V is specifically expressed in the brain, whereas ST6GalNAc-VI is expressed in a wide range of tissues. Furthermore, we determined the sialyltransferase responsible for the synthesis of disialyl Le a (disialyl Lewis a) to be ST6GalNAc-VI [21]. This structure contains a branched-type disialyl structure on a lacto-core structure.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification and expression of a sialyltransferase responsible for the synthesis of disialylgalactosylgloboside in normal and malignant kidney cells: downregulation of ST6GalNAc VI in renal cancers

Senda

Itoh

Tsuchida

et al. 2007

Biochemical Journal

Self Cite

View full text Add to dashboard Cite

Although disialyl glycosphingolipids such as GD3 and GD2 have been considered to be associated with malignant tumours, whether branched-type disialyl glycosphingolipids show such an association is not well understood. We investigated the sialyltransferases responsible for the biosynthesis of DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside). Among six GalNAc:α2,6-sialyltransferases cloned to date, we focused on ST6GalNAc III, V and VI, which utilize sialylglycolipids as substrates. In vitro enzyme analyses revealed that ST6GalNAc III and VI generated DSGG from MSGG with V max /K m values of 1.91 and 4.16 respectively. Transfection of the cDNA expression vectors for these enzymes resulted in DSGG expression in a renal cancer cell line. Although both ST6GalNAc III and VI genes were expressed in normal kidney cells, the expression profiles of ST6GalNAc VI among 20 renal cancer cell lines correlated clearly with those of DSGG, suggesting that the sialyltransferase involved in the synthesis of DSGG in the kidney is ST6GalNAc-VI. ST6GalNAc-VI and DSGG were found in proximal tubule epithelial cells in normal kidney tissues, while they were downregulated in renal cancer cell lines and cancer tissues. All these findings indicated that DSGG was suppressed during the malignant transformation of the proximal tubules as a maturation arrest of glycosylation.

show abstract

Section: Hst6galnac III and Vi Synthesize Dsggsupporting

confidence: 77%

Section: Flow Cytometrymentioning

confidence: 99%

See 1 more Smart Citation

Identification and expression of a sialyltransferase responsible for the synthesis of disialylgalactosylgloboside in normal and malignant kidney cells: downregulation of ST6GalNAc VI in renal cancers

Senda

Itoh

Tsuchida

et al. 2007

Biochemical Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, however, more direct evidence of the contribution of incomplete synthesis the enhanced expression of sialyl Lewis a/x has been accumulated; i.e., further modified forms of sialyl Lewis x and sialyl Lewis a determinants were found to be significantly expressed in non-malignant colonic epithelium, namely, sialyl 6-sulfo Lewis x 27) and disialyl Lewis a [45][46][47] (Fig. 7).…”

Section: The Concept Of Incomplete Synthesis and Induction Of Sialyl mentioning

confidence: 99%

Carbohydrate‐mediated cell adhesion in cancer metastasis and angiogenesis

et al. 2004

Self Cite

View full text Add to dashboard Cite

“…The human sialyltransferase ST6GalNac V, a member of the ST6GalNac family of sialyltransferases, is a type II Golgi membrane protein that transfers sialic acid from the donor CMP-Neu5Ac to the GalNac residue on the ganglioside, GM1b, forming GD1␣. Tsuchida et al (21) proposed indirect involvement of siat7e in synthesizing disialyl Le a , a carbohydrate structure conjugated to proteins and ceramides on the cell surface. In other studies, glycosphingolipids including gangliosides have been reported to mediate cell adhesion through the sugar residue interactions in the glycosynapse microdomains (22,23).…”

mentioning

confidence: 99%

Conversion of MDCK cell line to suspension culture by transfecting with human siat7e gene and its application for influenza virus production

Chu

Lugovtsev

Golding

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

MDCK cells are currently being considered as an alternative to embryonated eggs for influenza virus propagation and hemagglutinin (HA) production intended for vaccine manufacturing. MDCK cells were found suitable for the virus production but their inability to grow in suspension burdens the process of scale up and hence their production capability. Anchorage-dependent MDCK cells were converted to anchorage-independent cells, capable of growing in suspension as a result of transfection with the human siat7e gene (ST6GalNac V). This gene was previously identified as having an important role in cellular adhesion when the transcriptions of genes from anchorage-dependent and anchorage-independent HeLa cells were compared. Unlike the parental MDCK cells, the siat7e-expressing cells were capable of growing in shake flasks as suspension cultures, achieving maximum concentration of 7 ؋ 10 5 cells/mL while keeping close to 100% viability throughout the growth phase. In production experiments, the siat7e-expressing cells were infected with the Influenza B/Victoria/504/2000 strain. It was determined that the cell-derived viruses retained similar antigenic properties as those obtained from egg-derived viruses and their nucleotide sequences were identical. The specific production of hemagglutinin (expressed in hemagglutination units per 10 6 cells) from the siat7e-expressing cells was approximately 20 times higher than the specific production from the parental MDCK cells. If this suspension process scales up, the production potential of HA from 10 L of siat7e-expressing cells at a concentration of 10 6 cells/mL would be equivalent to the amount of HA obtained from 10,000 embryonated eggs.anchorage-independent ͉ hemagglutinin ͉ sialyltransferase ͉ vaccine

show abstract

Synthesis of Disialyl Lewis a (Lea) Structure in Colon Cancer Cell Lines by a Sialyltransferase, ST6GalNAc VI, Responsible for the Synthesis of α-Series Gangliosides

Abstract: Biosynthesis of disialyl Lewis a (Le

Cited by 89 publications

References 23 publications

Identification and expression of a sialyltransferase responsible for the synthesis of disialylgalactosylgloboside in normal and malignant kidney cells: downregulation of ST6GalNAc VI in renal cancers

Identification and expression of a sialyltransferase responsible for the synthesis of disialylgalactosylgloboside in normal and malignant kidney cells: downregulation of ST6GalNAc VI in renal cancers

Carbohydrate‐mediated cell adhesion in cancer metastasis and angiogenesis

Conversion of MDCK cell line to suspension culture by transfecting with human siat7e gene and its application for influenza virus production

Contact Info

Product

Resources

About