Plaque Reduction Neutralization Test (PRNT) in the Congenital Zika Syndrome: Positivity and Associations with Laboratory, Clinical, and Imaging Characteristics

Ribeiro, Marizélia Rodrigues Costa; Khouri, Ricardo; Sousa, Pablo; Branco, Maria R. F. C.; Batista, Rosângela Fernandes Lucena; Costa, Elaine P. F.; Alves, Maria Teresa Seabra Soares de Britto e; Amaral, Gláucio Andrade; Borges, Marcella Costa Ribeiro; Takahasi, Eliana Harumi Morioka; Gomes, Lillian Nunes; Mendes, Ana Karolina Torres; Cavalcante, Tamires Barradas; Costa, Luciana Cavalcante; Félix, Alvina Clara; Souza, Nathalia C. Santiago; Silva, Antônio Augusto Moura da

doi:10.3390/v12111244

Cited by 16 publications

(22 citation statements)

References 34 publications

(54 reference statements)

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“…Recently, Ribeiro et al published data from analyses on the positivity of PRNT and serological tests among a total of 88 children in northeastern Brazil who presented clinical cases of CZS between 2016 and 2018. Six (6.8%) were positive for IgG anti-ZIKV, and 2 (2.3%) of them were ZIKV PRNT 90 -positive ( 67 ). Although our initial goal was not to make correlations like those made by Ribeiro et al, our data are very close.…”

Section: Discussionmentioning

confidence: 99%

Differential Longevity of Memory CD4 and CD8 T Cells in a Cohort of the Mothers With a History of ZIKV Infection and Their Children

et al. 2021

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Background: Zika virus (ZIKV) infection causes for mild and self-limiting disease in healthy adults. In newborns, it can occasionally lead to a spectrum of malformations, the congenital Zika syndrome (CZS). Thus, little is known if mothers and babies with a history of ZIKV infection were able to develop long-lasting T-cell immunity. To these issues, we measure the prevalence of ZIKV T-cell immunity in a cohort of mothers infected to the ZIKV during pregnancy in the 2016–2017 Zika outbreak, who gave birth to infants affected by neurological complications or asymptomatic ones.Results: Twenty-one mothers and 18 children were tested for IFN-γ ELISpot and T-cell responses for flow cytometry assays in response to CD4 ZIKV and CD8 ZIKV megapools (CD4 ZIKV MP and CD8 ZIKV MP). IFN-γ ELISpot responses to ZIKV MPs showed an increased CD4 and CD8 T-cell responses in mothers compared to children. The degranulation activity and IFN-γ-producing CD4 T cells were detected in most mothers, and children, while in CD8 T-cells, low responses were detected in these study groups. The total Temra T cell subset is enriched for IFN-γ+ CD4 T cells after stimulation of CD4 ZIKV MP.Conclusion: Donors with a history of ZIKV infection demonstrated long-term CD4 T cell immunity to ZIKV CD4 MP. However, the same was not observed in CD8 T cells with the ZIKV CD8 MP. One possibility is that the cytotoxic and pro-inflammatory activities of CD8 T cells are markedly demonstrated in the early stages of infection, but less detected in the disease resolution phase, when the virus has already been eliminated. The responses of mothers' T cells to ZIKV MPs do not appear to be related to their children's clinical outcome. There was also no marked difference in the T cell responses to ZIKV MP between children affected or not with CZS. These data still need to be investigated, including the evaluation of the response of CD8 T cells to other ZIKV peptides.

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Section: Discussionmentioning

confidence: 99%

Differential Longevity of Memory CD4 and CD8 T Cells in a Cohort of the Mothers With a History of ZIKV Infection and Their Children

et al. 2021

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“…PRNT is frequently used to detect and determine the titer neutralizing antibodies and also to distinguish virus infections among related flaviviruses such as ZIKV and DENV [ 12 , 13 , 17 , 34 , 35 ]. The ZIKV PRNT evaluated here displayed adequate performance with sensitivity and specificity values above 93% regardless of previous DENV infection.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Two Serological Assays for Diagnosing Zika Virus Infection

et al. 2021

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Zika virus (ZIKV) emerged and spread rapidly in South American countries during 2015. Efforts to diagnose ZIKV infection using serological tools were challenging in dengue-endemic areas because of antigenic similarities between both viruses. Here, we assessed the performance of an in-house developed IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) and the plaque reduction neutralization test (PRNT) to diagnose ZIKV infection. Acute and convalescent paired serum samples from 51 patients who presented with clinical symptoms suggestive of an arbovirus illness in dengue-endemic areas of Honduras, Venezuela, Colombia and Peru were used in the assessment. Samples were tested for ZIKV, dengue and chikungunya virus using a variety of laboratory techniques. The results for the ZIKV-RNA screening and seroconversion detected by the microneutralization test were used to construct a composite reference standard. The overall sensitivity and specificity for the MAC-ELISA were 93.5% and 100.0%, respectively. Contrastingly, the overall sensitivity and specificity for the PRNT were 96.8% and 95.0%, respectively. Restricting the analysis according to IgM or neutralizing antibodies against dengue, the performances of both serological assays were adequate. The findings of this study reveal that the MAC-ELISA and PRNT would provide initial reliable laboratory diagnostic assays for ZIKV infection in dengue-endemic areas.

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Section: Zika Virus Plaque Reduction Neutralization Testmentioning

confidence: 99%

“…The positive samples were further analyzed for the neutralizing antibody (NAb) titers by the plaque reduction neutralization test (PRNT90) as described earlier (Russell et al, 1967;Ward et al, 2018;Ribeiro et al, 2020). The presence of neutralizing antibodies was determined as described by Russell et al with some modifications (Ribeiro et al, 2020). Vero CCL-81 cell suspension (1.0 × 10 5 /mL/well) was added into 24-well tissue culture plates and was incubated for 16-24 h at 37 • C with 5% CO 2 to obtain confluent monolayer.…”

Section: Zika Virus Plaque Reduction Neutralization Testmentioning

confidence: 99%

Zika a Vector Borne Disease Detected in Newer States of India Amidst the COVID-19 Pandemic

Yadav

Kaur²,

Gupta³

et al. 2022

Front. Microbiol.

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BackgroundDuring the second wave of the COVID-19 pandemic, outbreaks of Zika were reported from Kerala, Uttar Pradesh, and Maharashtra, India in 2021. The Dengue and Chikungunya negative samples were retrospectively screened to determine the presence of the Zika virus from different geographical regions of India.MethodsDuring May to October 2021, the clinical samples of 1475 patients, across 13 states and a union territory of India were screened and re-tested for Dengue, Chikungunya and Zika by CDC Trioplex Real time RT-PCR. The Zika rRTPCR positive samples were further screened with anti-Zika IgM and Plaque Reduction Neutralization Test. Next generation sequencing was used for further molecular characterization.ResultsThe positivity was observed for Zika (67), Dengue (121), and Chikungunya (10) amongst screened cases. The co-infections of Dengue/Chikungunya, Dengue/Zika, and Dengue/Chikungunya/Zika were also observed. All Zika cases were symptomatic with fever (84%) and rash (78%) as major presenting symptoms. Of them, four patients had respiratory distress, one presented with seizures, and one with suspected microcephaly at birth. The Asian Lineage of Zika and all four serotypes of Dengue were found in circulation.ConclusionOur study indicates the spread of the Zika virus to several states of India and an urgent need to strengthen its surveillance.

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Plaque Reduction Neutralization Test (PRNT) in the Congenital Zika Syndrome: Positivity and Associations with Laboratory, Clinical, and Imaging Characteristics

Cited by 16 publications

References 34 publications

Differential Longevity of Memory CD4 and CD8 T Cells in a Cohort of the Mothers With a History of ZIKV Infection and Their Children

Differential Longevity of Memory CD4 and CD8 T Cells in a Cohort of the Mothers With a History of ZIKV Infection and Their Children

Evaluation of Two Serological Assays for Diagnosing Zika Virus Infection

Zika a Vector Borne Disease Detected in Newer States of India Amidst the COVID-19 Pandemic

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