Plants used to treat snakebites in Santarém, western Pará, Brazil: An assessment of their effectiveness in inhibiting hemorrhagic activity induced by Bothrops jararaca venom

Moura, Valéria Mourão de; Freitas-de-Sousa, Luciana Aparecida; Dos-Santos, Maria Cristina; Raposo, Juliana Divina Almeida; Lima, Aline Evangelista; Oliveira, Rodrigo Santos de; Silva, Milton; Mourão, Rosa Helena Veras

doi:10.1016/j.jep.2014.12.020

Cited by 53 publications

(34 citation statements)

References 32 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…alternatus and B . jararaca snake venom-induced edema and skin hemorrhage [5, 11]. However, no studies have compared the abilities of K .…”

Section: Resultsmentioning

confidence: 99%

“…brasileinsis leaves pre-incubated with the B . jararaca venom was described recently, with inhibition of about 40% the diameter of the intradermal hemorrhagic halo [5]. …”

Section: Resultsmentioning

confidence: 99%

“…These species are indistinctively known by the same common name in Brazil as "saião" and "coirama," and are widely used in traditional medicine for their anti-inflammatory properties [3]. Several popular reports have indicated that these species could be useful for treating snakebites [4, 5, 6]. …”

Section: Introductionmentioning

confidence: 99%

“…In this scenario, the search for new complementary therapies to treat snakebites is relevant and medicinal plants could be highlighted as a rich source of natural inhibitors and pharmacologically active compounds [33, 34]. A significant number of plants have been evaluated for their potential inhibitory activity of Bothrops venoms [5, 33, 34, 35, 36, 37]. …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom

et al. 2016

View full text Add to dashboard Cite

The species Kalanchoe brasiliensis and Kalanchoe pinnata, both known popularly as “Saião,” are used interchangeably in traditional medicine for their antiophidic properties. Studies evaluating the anti-venom activity of these species are scarce. This study aims to characterize the chemical constituents and evaluate the inhibitory effects of hydroethanolic leaf extracts of K. brasiliensis and K. pinnata against local effects induced by Bothrops jararaca snake venom. Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography coupled with Diode Array Detection and Electrospray Mass Spectrometry (HPLC-DAD-MS/MS) were performed for characterization of chemical markers of the extracts from these species. For antiophidic activity evaluation, B. jararaca venom-induced paw edema and skin hemorrhage in mice were evaluated. In both models, hydroethanolic extracts (125–500 mg/kg) were administered intraperitoneally in different protocols. Inhibition of phospholipase enzymatic activity of B. jararaca was evaluated. The HPLC-DAD-MS/MS chromatographic profile of extracts showed some particularities in the chemical profile of the two species. K. brasileinsis exhibited major peaks that have UV spectra similar to flavonoid glycosides derived from patuletin and eupafolin, while K. pinnata showed UV spectra similar to flavonoids glycosides derived from quercetin and kaempferol. Both extracts significantly reduced the hemorrhagic activity of B. jararaca venom in pre-treatment protocol, reaching about 40% of inhibition, while only K. pinnata was active in post-treatment protocol (about 30% of inhibition). In the antiedematogenic activity, only K. pinnata was active, inhibiting about 66% and 30% in pre and post-treatment protocols, respectively. Both extracts inhibited phospholipase activity; however, K. pinnata was more active. In conclusion, the results indicate the potential antiophidic activity of Kalanchoe species against local effects induced by B. jararaca snake venom, suggesting their potential use as a new source of bioactive molecules against bothropic venom.

show abstract

“…alternatus and B . jararaca snake venom-induced edema and skin hemorrhage [5, 11]. However, no studies have compared the abilities of K .…”

Section: Resultsmentioning

confidence: 99%

“…brasileinsis leaves pre-incubated with the B . jararaca venom was described recently, with inhibition of about 40% the diameter of the intradermal hemorrhagic halo [5]. …”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Os resíduos catiônicos podem interagir com grupos aniônicos de um sítio receptor nos fosfolipídeos da membrana (LOMONTE et al, 2003), enquanto que os resíduos hidrofóbicos poderiam desestabilizar as membranas e permeá-la através de sua interação com seus fosfolipídeos (NÚÑEZ et al, 2001). Lomonte et al (2003) TICLI et al, 2005;NÚÑEZ et al, 2005;OLIVEIRA et al, 2005;MOURA, 2014MOURA, , 2015, no soro de mamíferos como Didelphis sp (Gambá) (TRENTO et al, 2001;ROCHA et al, 2002), e também de plasma de serpentes (LIZANO et al, 2003;QUIRÓS et al, 2007;SHIMADA et al, 2008;OLIVEIRA et al, 2008;SANTOS-FILHO et al, 2011;MOORJAN, 2014;PALACIO, 2014 Plathymenia reticulata e outras. (MARTZ, 1992;MORS et al, 2000;TICLI et al, 2005, OLIVEIRA et al, 2005HAGE-MELIM et al, 2013).…”

Section: Miotoxinasunclassified

Caracterização bioquímica e funcional de um inibidor recombinante de miotoxinas, do tipo-?, da serpente Bothrops alternatus

Sousa¹

View full text Add to dashboard Cite

, por ter aberto as portas do Laboratório de Toxinologia dando todo o suporte necessário para realização deste trabalho e pela valiosa orientação, contribuindo para minha formação acadêmica e profissional, muito obrigado. Aos meus pais, Lodovico e Rosa, pela compreensão, amor e por todos os esforços dedicados à minha formação, permitindo que eu atingisse este objetivo. Aos meus irmãos, Rafael e Natália, por me apoiarem e acreditarem no meu sucesso. Ao Norival e à Adélia, por todo suporte, por terem compartilhando comigo suas experiências e pela grande contribuição técnica a esta pesquisa.

show abstract

Four almost unexplored species of Brazilian Connarus (Connaraceae): Chemical composition by ESI‐QTof‐MS/MS–GNPS and a pharmacologic potential

Paim

Santos

Toledo

et al. 2021

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction Species of Connaraceae are globally used in traditional medicines. However, several of these have not been studied regarding their chemical composition, and some are even at risk of extinction without proper studies. Therefore, the chemical composition and pharmacological potential of Connarus blanchetii Planch., Connarus nodosus Baker, Connarus regnellii G. Schellenb., and Connarus suberosus Planch., which were previously unknown, were analyzed. Objective This work aims to investigate the pharmacological potential of these four Connarus species. The chemical composition of different extracts was determined by high‐resolution mass spectrometry (HRMS), with subsequent analysis by the GNPS platform and competitive fragmentation modeling (CFM). Materials and methods Leaf extracts (C. blanchetii, C. nodosus, C. regnellii, and C. suberosus) and bark extracts (C. regnellii and C. suberosus) were obtained by decoction, infusion, and maceration. LC/HRMS data were submitted to the GNPS platform and evaluated using CFM in order to confirm the structures. Results The HRMS–GNPS/CFM analysis indicated the presence of 23 compounds that were mainly identified as phenolic derivatives from quercetin and myricetin, of which 21 are unedited in the Connarus genus. Thus, from the analyses performed, we can identify different compounds with pharmacological potential, as well as the most suitable forms of extraction. Conclusion Using HRMS–GNPS/CFM, 21 unpublished compounds were identified in the studied species. Therefore, our combination of data analysis techniques can be used to determine their chemical composition.

show abstract

Plants used to treat snakebites in Santarém, western Pará, Brazil: An assessment of their effectiveness in inhibiting hemorrhagic activity induced by Bothrops jararaca venom

Cited by 53 publications

References 32 publications

Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom

Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom

Caracterização bioquímica e funcional de um inibidor recombinante de miotoxinas, do tipo-?, da serpente Bothrops alternatus

Four almost unexplored species of Brazilian Connarus (Connaraceae): Chemical composition by ESI‐QTof‐MS/MS–GNPS and a pharmacologic potential

Contact Info

Product

Resources

About