Plants for delivery of edible vaccines

Walmsley, Amanda Maree; Arntzen, Charles J.

doi:10.1016/s0958-1669(00)00070-7

Cited by 183 publications

(90 citation statements)

References 34 publications

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“…Therefore, oral delivery of properly folded and fully functional biopharmaceuticals should significantly cut down the production cost. Bioencapsulation of pharmaceutical proteins within plant cells offers protection against digestion in the stomach but allows successful delivery to the target tissues [40].…”

Section: Engineering the Chloroplast Genome To Develop Edible Vaccinesmentioning

confidence: 99%

Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology

Daniell

Khan

Allison

2002

Trends in Plant Science

322

153

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Chloroplast genomes defied the laws of Mendelian inheritance at the dawn of plant genetics, and continue to defy the mainstream approach to biotechnology, leading the field in an environmentally friendly direction. Recent success in engineering the chloroplast genome for resistance to herbicides, insects, disease and drought, and for production of biopharmaceuticals, has opened the door to a new era in biotechnology. The successful engineering of tomato chromoplasts for high-level transgene expression in fruits, coupled to hyper-expression of vaccine antigens, and the use of plant-derived antibiotic-free selectable markers, augur well for oral delivery of edible vaccines and biopharmaceuticals that are currently beyond the reach of those who need them most.Chloroplast transformation is an environmentally friendly approach to plant genetic engineering that minimizes out-crossing of transgenes to related weeds or crops [1,2] and reduces the potential toxicity of transgenic pollen to non-target insects [3]. Because the plastid genome is highly polyploid, transformation of chloroplasts permits the introduction of thousands of copies of foreign genes per plant cell, and generates extraordinarily high levels of foreign protein [3]. Chloroplast transformation vectors use two targeting sequences that flank the foreign genes and insert them, through homologous recombination, at a precise, predetermined location in the organelle genome (Fig. 1). This results in uniform transgene expression among transgenic lines and eliminates the 'position effect' often observed in nuclear transgenic plants. Gene silencing, frequently observed in nuclear transgenic plants, has not been observed in genetically engineered chloroplasts. The ability to express foreign proteins at high levels in chloroplasts and chromoplasts, and to engineer foreign genes without the use of antibiotic resistant genes [4,5],make this compartment ideal for the development of edible vaccines [6]. Moreover, the ability of chloroplasts to form disulfide bonds and to fold human proteins has opened the door to high-level production of biopharmaceuticals in plants [7]. Furthermore, foreign proteins observed to be toxic in the cytosol are non-toxic when accumulated within transgenic chloroplasts [6,8]. Chloroplast and nuclear genetic engineering are compared in Table 1.

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Section: Engineering the Chloroplast Genome To Develop Edible Vaccinesmentioning

confidence: 99%

Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology

Daniell

Khan

Allison

2002

Trends in Plant Science

322

153

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“…In comparison with the traditional expression systems, plant-derived hepatitis B vaccine has attracted the attention of the pharmaceutical industry for reasons of safety and economy (Walmsley & Arntzen 2000). In the experiment with mice and human beings, parenteral immunization of plantderived hepatitis B surface antigen stimulated B and T cell immune responses similar to those found with commercial yeast-derived hepatitis B vaccine (Thanavala et al 1995).…”

mentioning

confidence: 86%

Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens

Tian¹,

Sun²,

Lu³

et al. 2011

Czech J. Genet. Plant Breed.

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Abstract:The plant expression vector pBRSAg was constructed as suitable for transformation via Agrobacteriummediated approach. It contains all elements for plant expression, such as CaMV 35S promoter, both left and right border sequence for transferred DNA (T-DNA) in Agrobacterium, plant reporter gene gus, and plant selection marker gene hpt. The recombinant binary vector pBRSAg was transformed into Agrobacterium tumefaciens strains by using the freeze-thaw method. Tomato cotyledon explants were transformed by A. tumefaciens and plants were regenerated on selection medium. GUS staining, PCR and PCR-Southern analysis of the plants were positive. It was for the first time shown that the HBsAg (hepatitis B surface antigen) gene was introduced into tomato plants. The expression of transgene is under investigation.

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“…For example, the development of transgenic plants which express vaccine proteins against a number of infectious diseases has increasingly become of great interest (Walmsley and Arntzen, 2000;Hefferon, 2007;Streatfield and Howard, 2003;Ma et al 2005;Hefferon, 2006). However, a number of stumbling blocks have been identified.…”

Section: *Corresponding Authormentioning

confidence: 99%

Amplification of genome-integrated BeYDV replicons by transient expression of Rep in Arabidopsis thaliana

Yong-Sun¹,

Hefferon²

2007

Electron. J. Biotechnol.

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Plants for delivery of edible vaccines

Cited by 183 publications

References 34 publications

Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology

Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology

Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens

Amplification of genome-integrated BeYDV replicons by transient expression of Rep in Arabidopsis thaliana

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