Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens

Tian, Li; Sun, Jin; Lu, Zhao Hua; Liu, Qing

doi:10.17221/5/2011-cjgpb

Cited by 14 publications

(6 citation statements)

References 27 publications

(22 reference statements)

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“…Moreover, the specific activity of Ta1 protein produced by tomato plants was higher than that from the synthetic E. coli system. Some research demonstrated that tomato plants can be exploited for the production of hepatitis B surface antigen (Lou et al 2007;Baesi et al 2011;Li et al 2011). All of the above findings support the concept of using transgenic tomato plants as a model for edible vaccines or producing antibodies.…”

Section: Molecular Farming Of Tomatoesmentioning

confidence: 75%

“…Thus, the establishment of simple and efficient regeneration systems is a fundamental prerequisite of taking advantage of cell and tissue culture for genetic improvement (genetically transformed plants for commercial applications). The in vitro culture of the tomato has been successfully used in different biotechnological application including the clonal propagation of high-value commercial cultivars, virus-free plants, and genetic transformation (Namitha and Negi 2013;Hanus-Fajerska 2006;Li et al 2011;Yarra et al 2012).…”

Section: Tissue Cultures Of Tomatomentioning

confidence: 99%

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Tomato (Solanum lycopersicum L.) in the service of biotechnology

Gerszberg

Hnatuszko-Konka

Kowalczyk

et al. 2014

Plant Cell Tiss Organ Cult

150

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Originating in the Andes, the tomato (Solanum lycopersicum L.) was imported to Europe in the 16th century. At present, it is an important crop plant cultivated all over the world, and its production and consumption continue to increase. This popular vegetable is known as a major source of important nutrients including lycopene, bcarotene, flavonoids and vitamin C as well as hydroxycinnamic acid derivatives. Since the discovery that lycopene has anti-oxidative, anti-cancer properties, interest in tomatoes has grown rapidly. The development of genetic engineering tools and plant biotechnology has opened great opportunities for engineering tomato plants. This review presents examples of successful tissue culture and genetically modified tomatoes which resistance to a range of environmental stresses improved, along with fruit quality. Additionally, a successful molecular farming model was established.

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Section: Molecular Farming Of Tomatoesmentioning

confidence: 75%

Section: Tissue Cultures Of Tomatomentioning

confidence: 99%

Tomato (Solanum lycopersicum L.) in the service of biotechnology

Gerszberg

Hnatuszko-Konka

Kowalczyk

et al. 2014

Plant Cell Tiss Organ Cult

150

View full text Add to dashboard Cite

show abstract

“…They orally immunised mice by using lyophilised form of plant material and showed the presence of secretory IgA (S-IgA) and total serum antibodies. Li et al (2011) also demonstrated the transformation of HBsAg (hepatitis B surface antigen) gene in to tomato mediated by Agrobacterium tumifaciens.…”

Section: Hepatitis B Virusmentioning

confidence: 94%

Transgenic plants as green factories for vaccine production

Kumar¹,

Raja²,

Wani³

et al. 2013

Afr. J. Biotechnol.

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Edible vaccine technology represents an alternative to fermentation based vaccine production system. Transgenic plants are used for the production of plant derived specific vaccines with native immunogenic properties stimulating both humoral and mucosal immune responses. Keeping in view the practical need of new technology for production and delivery of inexpensive vaccines, especially in developing world, plant derived edible vaccines is the best option in hand to combat infectious diseases. Plant derived vaccine is easy to administer, cost effective, readily acceptable, have increased safety, stability, versatility and efficacy. Several plant derived vaccines are under research, some are under clinical trials for commercial use. Like most biotechnology products, the IP situation for edible vaccines is complex as IP rights influence every stage of vaccine development.

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“…The antigen produced was recognizable by rabbit anti-TonB antiserum and suitable to be used as vaccine against Helicobacter infections by oral administration. In addition, Li et al showed that hepatitis B surface antigen gene was able to be introduced into tomato plants mediated by A. tumefaciens [42]. Agrobacterium-mediated transformation has also been used for the production of antigens such as heatlabile enterotoxin from Escherichia coli, Norwalk virus capsid protein, hepatitis B surface antigen, and transgenic alfalfa expressing proteins from the foot and mouth disease virus by using potato model [43].…”

Section: Agrobacterium-mediated Genementioning

confidence: 99%

Plant-Based Vaccines: Production and Challenges

et al. 2016

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Plant-based vaccine technologies involve the integration of the desired genes encoding the antigen protein for specific disease into the genome of plant tissues by various methods. Agrobacterium-mediated gene transfer and transformation via genetically modified plant virus are the common methods that have been used to produce effective vaccines. Nevertheless, with the advancement of science and technology, new approaches have been developed to increase the efficiency of former methods such as biolistic, electroporation, agroinfiltration, sonication, and polyethylene glycol treatment. Even though plant-based vaccines provide many benefits to the vaccine industry, there are still challenges that limit the rate of successful production of these third-generation vaccines. Even with all the limitations, continuous efforts are still ongoing in order to produce efficient vaccine for many human and animals related diseases owing to its great potentials. This paper reviews the existing conventional methods as well as the development efforts by researchers in order to improve the production of plant-based vaccines. Several challenges encountered during and after the production process were also discussed.

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Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens

Cited by 14 publications

References 27 publications

Tomato (Solanum lycopersicum L.) in the service of biotechnology

Tomato (Solanum lycopersicum L.) in the service of biotechnology

Transgenic plants as green factories for vaccine production

Plant-Based Vaccines: Production and Challenges

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