Plantlet regeneration from leaf derived callus of Vanilla planifolia Andr.

Abstract

Vanilla planifolia is a tropical orchid mainly known for the aromatic flavor of its cured pods. Callus cultures were initiated from leaf and nodal explants of V. planifolia. Leaf explants showed better callus initiation than the nodal explants with callus biomass production maximal when cultured on Murashige and Skoog (MS) basal medium containing 4.52 mM 2,4-dichlorophenoxy acetic acid and 2.22 mM benzyladenine (BAP). Callus transferred to MS basal medium supplemented with 13.32 μM BAP, and 13.43 μM naphthaleneacetic acid (NAA) showed superior growth response and produced 14.0±1.0 shoots with an average length of 3.6 ± 0.1 cm after 40 d. Subsequent transfer of the proliferated shootlets to MS basal medium supplemented with 8.88 μM BAP and 8.08 μM NAA produced 12.3±0.14 plantlets with an average height of 3.6 cm±0.10 cm. All plantlets produced profuse rooting within 35-40 d after transfer to halfstrength MS basal medium supplemented with NAA in combination with indole-3-acetic acid. Rooted plantlets were transferred for hardening, with 80% of the plantlets becoming successfully established in the field. Potentially, more than 100,000 plantlets could be produced within eight subcultures from callus obtained from leaf explant through the methods described here.

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

“…Among them are Aerides (Devi, Devi, & Singh, 2013), Aranda (Gantait, Bustam, & Sinniah, 2012), Phalaenopsis (Kuo, Chen, & Chang, 2005;Khoddamzadeh et al, 2011), Vanda and Dendrobium (Martin & Madassery, 2006;Tee, Wong, Lam, & Maziah, 2010), and Oncidium (Mayer, Stancato, & Appezzato-Da-Glória, 2010). In leaf cultures, for example in Renanthera (Wu et al, 2012) and Vanilla (Janarthanam & Seshadri, 2008), wound surface of leaf explants had been reported to produce callus. Wu et al, (2012) reported that 99.67 % plantlet formation was obtained from callus originated from leaf segments of Renanthera orchid.…”

In Vitro Mass Propagation of Ornamentally and Medicinally Important Coelogyne flaccida Lindl. through Pseudobulb Segments

“…Orchids are propagated in vitro by using various explants such as shoot meristem, leaves, roots, protocorms etc. obtained from axenic cultures but the regenerative potential of pseudobulbs as explants has been less explored as compared to the other explants (Morel 1960, 1970, Stewart and Button 1976, Vajrabhaya 1978, Shimasaki and Uemoto 1987, Arditti and Ernst 1993, George and Ravishankar 1997, Vij and Kaur 1998, Kanjilal et al 1999, Pyati et al 2002, Decruse et al 2003, Basker and Narmatha 2006, Martin 2007, Janarthanam and Seshadri 2008, Medina et al 2009, Sungkum and Deb 2009, Sunitibala and Kishor 2009, Hong et al 2010, Mata-Rosas et al 2010, Kaur and Bhutani 2010, Rajkarnikar 2011, Pant and Thapa 2012. Therefore, an attempt was made to establish an efficient regeneration system by using pseudobulbs as explants for C. flaccida in particular checking the (i) influence of maturity level of pseudobulbs on regeneration potential, (ii) suitable culture medium, and the effect of growth regulators individually and in combination on regeneration of the explants and their successive development into plantlets (iii) induction potential of the apical and basal segments.There is no report on the development of in vitro methodology for mass propagation of C. flaccida using different explants.…”

Importancia y desafíos de la conservación de Vanilla spp. (Orquidaceae) en Costa Rica.

“…Vanilla planifolia es cultivada comercialmente por sus frutos (cápsulas) de los cuales se extrae la sustancia aromatizante y saborizante llamada vainillina (George y Ravishankar, 1997;Geetha y Shetty, 2000;Giridhar y Ravishankar, 2004;Pak et al, 2004;Kalimuthu et al, 2006;Havkin-Frenkel y Belanger, 2007;Janarthanam y Seshadri, 2008;Chandran y Puthur, 2009;González-Arnao et al, 2009;Tan et al, 2010;Hailemichael et al, 2012). De los más de 250 compuestos diferentes aislados de los frutos de V. planifolia, la vainillina es el componente principal de las cápsulas fermentadas y es el sabor más ampliamente distribuido en el mundo (Pak et al, 2004;HavkinFrenkel y Belanger, 2007).…”