Plantlet regeneration from different callus cultures of pigeonpea (Cajanus cajan L.)

Kumar, Arun; Reddy, T. P.; Reddy, G. M.

doi:10.1016/0304-4211(83)90032-9

Cited by 49 publications

(20 citation statements)

References 11 publications

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“…Eapen and George (1993) obtained regeneration of calli from the leaf discs of pigeon pea and peanut, and indicated that BAP + IAA or IAA + aspartic acid are essential for shoot bud elongation. In addition, Kumar et al (1983) reported regeneration from the leaf callus of pigeon pea on Blaydes (1966) medium with 0.5 mg L −1 BAP, 0.1 mg L −1 NAA, and 0.01 mg L −1 GA3.…”

Section: Shoot Bud Elongationmentioning

confidence: 99%

“…In pigeon pea, the regeneration of plants by organogenesis has been reported via pre-existing meristems like apical meristem (Cheema and Bawa 1991), undifferentiated callus (Kumar et al 1983;George and Eapen 1994), differentiated non-meristematic leaf tissue Eapen et al 1998;Geetha et al 1998;Singh et al 2002;Dayal et al 2003;Villiers et al 2008); and seedling tissues such as hypocotyl (Cheema and Bawa 1991;Geetha et al 1998), cotyledon (George and Eapen 1994;Geetha et al 1998;Mohan and Krishnamurthy 1998;Chandra et al 2003), cotyledonary node (Mehta and Mohan Ram 1980;Kumar et al 1983Kumar et al , 1984Naidu et al 1995;Shiva Prakash et al 1994;Geetha et al 1998;Singh et al 2003), epicotyl (Kumar et al 1984;Naidu et al 1995;Geetha et al 1998), and embryonal axes (Sarangi and Gleba 1991;George and Eapen 1994;Naidu et al 1995;Franklin et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea cv. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L −1 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observed on half-strength Murashige and Skoog (MS) medium with 2 % sucrose and 0.5 mg L −1 3-indolebutyric acid (IBA). On the other hand, the regeneration of globular embryos from cotyledon explant was faster and greater with thidiazuron (TDZ) than BAP with sucrose as carbohydrate source. These globular embryos were maturated on MS medium with abscisic acid (ABA) and finally germinated on half-strength MS medium at lower concentrations of BAP. Comparison of regeneration pathways in pigeon pea cv. JKR105 showed that the turnover of successful establishment of plants achieved through organogenesis was more compared to somatic embryogenesis, despite the production of more embryos than shoot buds.

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Section: Shoot Bud Elongationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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“…Shoot bud induction from cotyledonary callus of pigeon pea on Blaydes medium with 2.25 mgL -1 BAP has been reported (Kumar et al, 1983). Similarly, 65% shoot buds from cotyledonary node explants of cultivar T-15-15 and SPMA-4 has been reported (Shiva et al, 1994;Mohan and Krishnamurthy, 1998).…”

Section: Resultsmentioning

confidence: 92%

“…In vitro regeneration by organogenesis using diverse explants like leaf discs (Kumar et al, 1983;Eapen and George, 1993;George and Eapen, 1994;Eapen et al, 1998;Geetha et al, 1998;Dayal et al, 2003), cotyledons (Kumar et al, 1983;Mehta and Mohan, 1980;Kumar et al, 1984;Sarangi and Gleba, 1991;Naidu et al, 1995;Srinivasan et al, 2004;Chandra et al, 2003), cotyledonary nodes (Geetha et al, 1998;Shiva et al, 1994) and embryonal axes (George and Eapen, 1994;Sarangi and Gleba, 1991;Naidu et al, 1995;Franklin et al, 2000;Mohan and Krishnamurthy, 1998) has been reported. More than 50 diverse genotypes have been attempted for regeneration via organogenesis to develop suitable regeneration protocol amenable to transformation with desired genes (Krishna et al, 2010).…”

mentioning

confidence: 99%

In vitro multiple shoot bud induction and regeneration from plumule junction explants of pigeon pea [Cajanus cajan (L.) Mill sp.] cultivars

Kashyap¹,

Sarangi²,

Yadav³

et al. 2014

Afr. J. Biotechnol.

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“…However, reports of organogenesis from an unorganized callus are rare, except two (Kumar et al, 1983;George and Eapen, 1994). Organogenesis response of pigeon pea is genotype-dependent, besides various other factors like explant tissues, media composition, and plant growth regulators.…”

Section: Introductionmentioning

confidence: 99%

In vitro callus induction and regeneration of multiple shoots through callus derived from leaf and epicotyl explants in pigeon pea (Cajanus cajan L.)

Padmavathi

A.V.Thangella

Fakrudin

2017

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An efficient in vitro protocol was developed for callus induction, high frequency plant regeneration through callus cultures derived from cotyledonary leaf and epicotyl explants, rooting of shoots derived from callus and establishment onto the natural conditions in two cultivars of pigeon pea; ICPL 87119 and ICPL 8863. Cotyledonary leaf and epicotyl explants were tested for callus induction across 48 different combinations and concentrations of auxins and cytokinins in MS medium, wherein, higher doses of auxins (15 mg/1 NAA) in combination with lower doses of cytokinins (0.5 mg/l kinetin) induced regenerable callus from leaf explants while lower doses of auxins (0.2 mg/1 NAA) in combination with higher doses of cytokinins (8 mg/1 kinetin) induced regenerable callus from epicotyl explants in both the genotypes. Plantlet regeneration from leaf and epicotyl derived callus was optimized at 0.05 mg/l TDZ in both genotypes. Rooting was optimized on ½ MS + 0.5 mg/1 IBA media in both genotypes. Well-rooted plants were acclimatized and established successfully into natural conditions in potting mixture-containing soil: FYM in 1:1 ratio resulting in 48.01 per cent survivability. Regenerated plants were uniform morphologically with normal leaf shape and growth. This protocol finds its significance in rapid multiplication of transgenic plants.

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Plantlet regeneration from different callus cultures of pigeonpea (Cajanus cajan L.)

Cited by 49 publications

References 11 publications

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

In vitro multiple shoot bud induction and regeneration from plumule junction explants of pigeon pea [Cajanus cajan (L.) Mill sp.] cultivars

In vitro callus induction and regeneration of multiple shoots through callus derived from leaf and epicotyl explants in pigeon pea (Cajanus cajan L.)

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