Plantlet regeneration and propagation from ginger callus

Malamug, Josue Jack F.; Inden, Haruhisa; Asahira, Tadashi

doi:10.1016/0304-4238(91)90155-r

Cited by 29 publications

(7 citation statements)

References 10 publications

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“…Type 2 calluses produced on various media were capable only of rhizogenesis. Malamug et al (1991) have reported that the organogenic capability of soft callus of ginger is lost after three subcultures. Lack of shoot regeneration from type 2 callus in the present study may be due to either noncompetence or the age of the callus (5-6 months).…”

Section: Resultsmentioning

confidence: 99%

“…Tissue culture investigations in ginger are mostly confined to micropropagation from shoot-tip culture (Bhagyalakshmi & Singh 1988;Noguchi & Yamakawa 1988;Wang 1989;Balachandran et al 1990) and germplasm conservation (Dekkers et al 1991). Only recently Malamug et al (1991) have reported plant regeneration by organogenesis in ginger. Here we report a protocol for the efficient and reliable regeneration of plants through somatic embryogenesis using cultured leaf segments of ginger.…”

Section: Introductionmentioning

confidence: 99%

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Plant regeneration via somatic embryogenesis in ginger

Kackar¹,

Bhat²,

Chandel³

et al. 1993

Plant Cell Tiss Organ Cult

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Plant regeneration via somatic embryogenesis in ginger

Kackar¹,

Bhat²,

Chandel³

et al. 1993

Plant Cell Tiss Organ Cult

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“…The addition of vermiculite, a textured substrate, to rooting medium was found to improve root induction in multiple hardwood species, including walnut (Vahdati et al, 2004), papaya (Kaity et al, 2009), and pear (Tetsumura et al, 2011). Vermiculitecontaining medium also enhanced root morphogenesis in ginger and ginseng (Inden et al, 1988;Malamug et al, 1991;Shoyama et al, 1995), and improves rooting and root quality in specific apple cultivars (Druart, 1997;Navatel and Bourrain, 1994). We examined whether a gelling agent (phytagel) or activated charcoal were necessary when using vermiculite for rooting american chestnut shoots.…”

mentioning

confidence: 99%

Improving Rooting and Shoot Tip Survival of Micropropagated Transgenic American Chestnut Shoots

Oakes

Desmarais

Powell

et al. 2016

horts

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Many hardwood tree species are being threatened by exotic pests, and for some, only genetic engineering can offer a solution before functional extinction occurs. An example of how genetic engineering can be a useful tool for forest restoration is the transgenic american chestnuts, which contain a wheat oxalate oxidase gene conferring resistance to the chestnut blight. Many hundreds of these trees are needed for field trials and eventual restoration plantings throughout its natural range, but production is bottlenecked because of the difficulty of making hardwood trees produce roots through micropropagation. The presence of roots and living shoot tips precede successful acclimatization of tissue culture-produced american chestnut plantlets. In these experiments, we attempted to improve the post-rooting stage of our american chestnut propagation protocol. We examined vessel type, hormone, and activated charcoal concentrations, and using a vermiculite substrate. For plantlets with the best combination of roots and living shoot tips we recommend using semisolid post-rooting medium containing 2 g·L−1 activated charcoal and 500 mg humic acid in disposable fast-food takeout containers. When using vermiculite as a substrate, adding 2.0 g·L−1 activated charcoal to post-rooting medium without a gelling agent was the preferred treatment. Improving the survival rates of the american chestnut plantlets will benefit the american chestnut restoration project by providing more plant material for both ecological studies and eventual restoration, since pursuit of a nonregulated status for these transgenic trees will require extensive field testing. These procedures may also be applicable to other difficult-to-root hardwood trees in transgenic programs, such as american butternut, white oak, and black walnut.

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“…Significant differences (p<0.05) were detected among the effect of different concentrations of 2,4-D on callus induction. Resmi and Shylaja (2010) showed that 1.00 mg/L 2,4-D recorded the highest callusing (56.37%) and callus growth by Z. officinale Rosc shoot tip explants, also Ma and Gang (2006) reported the presence of 2,4-D at 0.5 to 1.5 mg/L in the culture medium resulted in callus growth for Z. officinale Rosc explants, moreover Malamug et al (1991) and Babu et al (1992) reported that 2,4-D is the most effective auxin for callus induction in ginger and turmeric.…”

Section: Callus Inductionmentioning

confidence: 99%