Plantlet Morphogenesis and the Control of Callus Growth and Root Induction of Phaseolus vulgaris with the Addition of a Bean Seed Extract

Crocomo, Otto J.; Sharp, W. R.; Peters, J. E.

doi:10.1016/s0044-328x(76)80094-3

Cited by 34 publications

(10 citation statements)

References 6 publications

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“…However, many plants, including important crops and species widely used for fundamental studies, like many legumes such as Phaseolus vulgaris, are generally recalcitrant to shoot regeneration. Shoot and plant regeneration in P. vulgaris in vitro has been attempted repeatedly, the first report being that of Crocomo et al (1976) who * To whom correspondence should be addressed Abbreviations: BAP = Nr-benzylaminopurine; MS = Murashige and Skoog (1962) medium; TDZ=N-phenyl-Nl-(1,2,3 thiadiazol-yl)urea (thidiazuron) recovered two plants from callus cultures using a medium supplemented with growth regulators and an aqueous bean-seed extract. Later, some regeneration of plants was obtained from apical and axillary meristems, and differentiation of proembryos was observed in cell-suspension cultures (Kartha et al 1981;Martins and Sondahl 1984a, b;Allavena 1984;Morginski and Kartha 1985).…”

Section: Introductionmentioning

confidence: 99%

Regeneration in Phaseolus vulgaris L.: High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron

Malik

Saxena

1992

Planta

191

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Induction of prolific shoot formation in Phaseolus vulgaris L. cv. Kinghorn Wax was achieved by germinating mature seeds and growing seedlings on a medium supplemented with 10 μM thidiazuron (TDZ), a substituted phenylurea, or 80 μM N(6)-benzylaminopurine (BAP). Culture for 7 d in the presence of 10 μM TDZ was sufficient to induce maximal shoot formation, whereas a continuous presence of BAP was required for the induction and development of shoots. The differentiation of adventitious shoots occurred within four weeks of seed culture, from tissues in the regions of axillary buds on the cotyledonary node and also areas surrounding the shoot apex of the intact seedling. The number of shoots regenerated from intact seedlings was significantly higher than that obtained with expiants. Regenerated shoots developed into flowering plants. Similar results were obtained in six other bean cultivars.

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Section: Introductionmentioning

confidence: 99%

Regeneration in Phaseolus vulgaris L.: High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron

Malik

Saxena

1992

Planta

191

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“…In vitro plant regeneration from pieces of bean leaves (P. vulgaris L.) was reported first by Crocomo et al (1976). Two plantlets were induced in a test of 9 cultures.…”

Section: Introductionmentioning

confidence: 99%

Factors affecting morphogenesis from immature cotyledons of Phaseolus coccineus L.

Genga

Allavena

1991

Plant Cell Tiss Organ Cult

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Direct somatic embryogenesis as well as somatic embryogenesis and organogenesis mediated by small glossy calluses were obtained from immature cotyledon explants of bean (P. coccineus) cv Streamline 770 on a modified half-st~rength MS medium (Murashige & Skoog 1962) containing various concentrations of (2-isopentenyl)adenine and 2-naphthoxyacetic acid. Substitution of sucrose with glucose gave, in the range of concentrations tested, the strongest enhancement of the morphogenic process. Further improvement regarding the number of morphogenic cotyledons, the number of regenerations per cotyledon and the quality of the embryos was observed when 2,3,5-triiodobenzoic acid or abscisic acid were added to the medium. After cycles of micropropagation on MS medium plus 4.4/zM 6-benzyladenine and rooting in the absence of growth factors, plantlets were adapted to ex vitro conditions and grown to maturity.

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“…Primary leaves including the intact petiole regenerated shoots via callussing in cowpea (Muthukumar et al, 1995). Addition of seed extract improved callussing and regeneration in microsperma lentil, as it had been reported to improve regeneration in case of Phaseolus vulgaris L., where plants were regenerated on a medium containing bean seed extract of an undefined composition (Crocomo et at., 1976). Cotyledonary node explain works well with the mungbean regeneration and the number of attached cotyledons and removal of a portion of one or both cotyledons affected shoot formation (Gulati and Jaiwal, 1994).…”

Section: Discussionmentioning

confidence: 93%

Influence of Microsperma Lentil Genotypes on Tissue Culture Responses

Altaf¹,

Iqbal²,

Ahmad³

1998

Pakistan J. of Biological Sciences

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In vitro growth responses of various lentil genotypes varied. The best callussing response was noticed in variety ILL-4605. The genotype AARI-L-89503 had a tendency for embryogenesis. The genotype AARI-L-86642 had the best rooting ability. This genotype also showed in vitro flowering response. Masoor-85 and Pant-L-406 had moderate morphogenic responses, Meristem multiplication, shoot development, shoot bud formation from callus and adventitious rooting from shoot cuttings was obtained under appropriate growth regulators combinations.

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Plantlet Morphogenesis and the Control of Callus Growth and Root Induction of Phaseolus vulgaris with the Addition of a Bean Seed Extract

Cited by 34 publications

References 6 publications

Regeneration in Phaseolus vulgaris L.: High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron

Regeneration in Phaseolus vulgaris L.: High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron

Factors affecting morphogenesis from immature cotyledons of Phaseolus coccineus L.

Influence of Microsperma Lentil Genotypes on Tissue Culture Responses

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