Plantibodies: A Novel Strategy to Create Pathogen-Resistant Plants

Liao, Yu-Cai; Li, Heping; Zhao, Chun-Sen; Yao, Mingjing; Zhang, Jingbo; Liu, Jinlong

doi:10.1080/02648725.2006.10648087

Cited by 5 publications

(2 citation statements)

References 75 publications

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“…In addition they carry two heavy chains and two light chains, and thus it is difficult to genetically manipulate them to construct fusion proteins with other partners. Rapid progress in molecular immunology, combined with the polymerase chain reaction, has made it possible to clone the antibody binding domain (Fv fragment) and express the polypeptide chains in bacteria, yeast, mammalian cells and plant cells either as pure antibodies or as fusion proteins comprising antibodies genetically linked to other peptides [ 29 , 30 ]. By advanced technologies such as phage display, antibody fragments specific for particular antigens can be isolated in vitro from libraries containing diverse repertoires of antibodies V-genes, which bypasses hybridoma technology altogether and generates single-chain antibodies with specificity and affinity similar to monoclonal antibodies.…”

Section: Antibodies Specific For Fusarium Mycotmentioning

confidence: 99%

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Antibody-mediated Prevention of Fusarium Mycotoxins in the Field

Zhang

et al. 2008

IJMS

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Fusarium mycotoxins directly accumulated in grains during the infection of wheat and other cereal crops by Fusarium head blight (FHB) pathogens are detrimental to humans and domesticated animals. Prevention of the mycotoxins via the development of FHB-resistant varieties has been a challenge due to the scarcity of natural resistance against FHB pathogens. Various antibodies specific to Fusarium fungi and mycotoxins are widely used in immunoassays and antibody-mediated resistance in planta against Fusarium pathogens has been demonstrated. Antibodies fused to antifungal proteins have been shown to confer a very significantly enhanced Fusarium resistance in transgenic plants. Thus, antibody fusions hold great promise as an effective tool for the prevention of mycotoxin contaminations in cereal grains. This review highlights the utilization of protective antibodies derived from phage display to increase endogenous resistance of wheat to FHB pathogens and consequently to reduce mycotoxins in field. The role played by Fusarium-specific antibody in the resistance is also discussed. OPEN ACCESSInt. J. Mol. Sci. 2008, 9 1916

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Section: Antibodies Specific For Fusarium Mycotmentioning

confidence: 99%

“…Expression levels observed in the transient assay are very consistent with those obtained with transgenic approach. We routinely use this system to test expression of antibodies or genes of interest, and to study their functions before moving to generating transgenic plants [ 29 , 55 ].…”

Section: Antibody Expression In Plantsmentioning

confidence: 99%