Plantform Bioreactor for Mass Micropropagation of Date Palm

Almusawi, Abdulminam Hussien; Sayegh, A. J.; Alshanaw, Ansam M. S.; Griffis, John L.

doi:10.1007/978-1-4939-7156-5_21

Cited by 11 publications

(2 citation statements)

References 7 publications

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“…Meanwhile, bioreactors are utilized in bioprocessing industries, wherein the optimum environmental conditions are maintained to achieve the required biological products on a large scale. The advantages of bioreactors include a better rate of product multiplication, lesser time for multiplication and a minimum cost 49 .…”

Section: Phytochemicals As Nutraceuticals: Phytochemicals Have Beenmentioning

confidence: 99%

In vitro Production of Plant Nutraceuticals

Bekheet¹,

Sota²

2022

Biotechnology

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Biotechnology methods particularly tissue culture techniques offer new approaches for the in vitro production of valuable plant nutraceuticals. The large-scale production of secondary metabolites in a year-round system without seasonal constraints is one of the advantages of plant tissue culture techniques. Furthermore, enhancement of in vitro nutraceuticals production can be realized by a selection of high-producing cell lines and medium optimization. In this respect, several techniques have been adopted to improve the production of plant-derived nutraceuticals such as mutation, elicitation, precursor feeding, genetic transformation and metabolic engineering. In vitro mutagenesis of cultured cells and tissues by irradiation or chemical mutagens represents a feasible method for the improvement of nutraceuticals production. Likewise, transformation is another strategy that is used to enhance nutraceuticals in plant cells or organ tissues. At this point, hairy root culture induced by Agrobacterium rhizogenes is the most promising transformation technique used for in vitro production of valuable plant compounds. The benefits of hairy roots are high metabolite productivity, high growth rates and inherent genetic stability. On the other hand, due to its effectiveness and practical feasibility, elicitation is considered the most applied strategy for enhancing the production of desired compounds in plant biotechnology. Biotic (biological origin) and abiotic (non-biological origin) elicitors are used to increase the production of plant metabolites by different effects on the cellular processes in the plant system. Furthermore, bioreactors developed for in vitro industrial-scale production of desired compounds can be used for continuous and scaling-up plant nutraceuticals production. This article discusses different aspects of biotechnology used for the selection and enhancement production of nutraceuticals from plant cultures grown cells, in vitro.

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Section: Phytochemicals As Nutraceuticals: Phytochemicals Have Beenmentioning

confidence: 99%

In vitro Production of Plant Nutraceuticals

Bekheet¹,

Sota²

2022

Biotechnology

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“…Bioreactors are now emerging implements in bioprocessing industries, wherein the optimum environmental conditions are maintained to achieve the required biological products on a large scale. The advantages of bioreactors include a better rate of product multiplication, lesser time for multiplication, and minimum cost [50]. Ginsenoside production in various bioreactors, under different culture conditions, is presented in Table 3.…”

Section: Bioreactor: Large-scale Propagationmentioning

confidence: 99%

Biotechnological Interventions for Ginsenosides Production

2020

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Ginsenosides are secondary metabolites that belong to the triterpenoid or saponin group. These occupy a unique place in the pharmaceutical sector, associated with the manufacturing of medicines and dietary supplements. These valuable secondary metabolites are predominantly used for the treatment of nervous and cardiac ailments. The conventional approaches for ginsenoside extraction are time-consuming and not feasible, and thus it has paved the way for the development of various biotechnological approaches, which would ameliorate the production and extraction process. This review delineates the biotechnological tools, such as conventional tissue culture, cell suspension culture, protoplast culture, polyploidy, in vitro mutagenesis, hairy root culture, that have been largely implemented for the enhanced production of ginsenosides. The use of bioreactors to scale up ginsenoside yield is also presented. The main aim of this review is to address the unexplored aspects and limitations of these biotechnological tools, so that a platform for the utilization of novel approaches can be established to further increase the production of ginsenosides in the near future.

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Optimization of cultivation conditions of Salvia viridis L. shoots in the Plantform bioreactor to increase polyphenol production

Grzegorczyk-Karolak

Staniewska

Lebelt

et al. 2021

Plant Cell Tiss Organ Cult

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Salvia viridis is a rich source of pharmacologically-important polyphenolic compounds with anti-inflammatory, antioxidant and anticancer activities. The aim of the present study was to optimise the large-scale cultivation of sage shoots in the Plantform bioreactor. It evaluated the effect of culture duration (two or three weeks), the use of a semi-continuous system and the immersion frequency in a liquid medium (every 80 or 170 min) on the growth and accumulation of secondary metabolites. The content of bioactive phenolic acids and phenylethanoids in the obtained material was determined using HPLC. The most efficient growth parameters, expressed as fresh (26.99 g) and dry weight (3.51 g) as well as proliferation ratio (18.6), were observed for shoots grown in a fed-batch system, immersed every 80 min; this approach yielded a 33-fold biomass increase within four weeks. However, the most efficient production of bioactive compounds was achieved for shoots grown for three weeks in a batch system, immersed every 80 min: total phenolic acid, total phenylethanoid and total phenol contents of 18.3, 11.4 and 29.7 mg/g DW. In these conditions, within three weeks, total phenolic acid level was almost 10 times higher than that found in the aerial parts of four-month-old soil-grown plants with a similar phenylethanoid level. Hence, the described S. viridis shoot culture cultivated in the Plantform bioreactor may be an alternative efficient source of plant material rich in valuable compounds.

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Plantform Bioreactor for Mass Micropropagation of Date Palm

Cited by 11 publications

References 7 publications

In vitro Production of Plant Nutraceuticals

In vitro Production of Plant Nutraceuticals

Biotechnological Interventions for Ginsenosides Production

Optimization of cultivation conditions of Salvia viridis L. shoots in the Plantform bioreactor to increase polyphenol production

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