Plant Volatiles Providing Additional Evidences to the Occurence of a Wild‐Growing Population of Calamintha vardarensis (Greuter et Burdet) Šilić Outside of Its Natural Habitat

Abstract

The essential-oil profile of a Calamintha species, wild-growing in the urban settings of the city of Niš (South Serbia) and botanically tentatively identified as C. vardarensis (endemic species native to FYR Macedonia and East Serbia), has been statistically (multivariate statistical analyses) compared with those of other Calamintha species, including two previously investigated C. vardarensis populations, as a means of corroboration of the surprising occurence of this Calamintha population outside of its natural distributional range. Agglomerative hierarchical clustering reveals a close link of C. vardarensis from Niš (with neo-menthol (40.0%), menthone (21.8%), and pulegone (27.2%) as its major oil contributors) and C. vardarensis from FYR Macedonia.

CommercialCarlinae radixherbal drug: Botanical identity, chemical composition and antimicrobial properties

“…As previously mentioned, the commercial drug C. radix sometimes contains roots of C. acanthifolia, which is considered both as an adulterant and a substitute of C. acaulis roots (Đorđević et al, 2005). It has been shown that in some cases, the essential oil profile can be used to confirm the botanical identification of plant taxa (Radulović & Blagojević, 2010b). For that reason, we compared the chemical composition of the herein studied C. radix essential oil with the composition of the previously investigated C. acanthifolia and C. acaulis oils (Table 1).…”

Chemical Composition of Hypericum rumeliacumBoiss. Essential Oil. A New Chemotype of This Pharmacologically Valuable Species?

“…Moreover, since plants in different phenological phases of development could potentially give rise to different VSM profiles, and the production and accumulation of secondary metabolites are strongly plant part (organ)-dependent, these also have to be taken into account [8] [9]. The same is true for different subspecies or even varieties of the same species [7].…”

Application of HS-SPME–GC-MS-derived variables for monitoring ripening-induced changes in cheese volatile compounds

“…Separation and identification of the volatile compounds extracted as described above were performed by GC-MS. The instruments used and the experimental conditions are identical to those described in Radulović and Blagojević (2010), except that the samples were manually injected by inserting the saturated fiber into the inlet liner for 10 min. The GC oven temperature was held at 45°C for 5 min, then raised to 250°C at a heating rate of 10°C.min −1 , and then held isothermally for 10 min at 250°C (carrier gas: helium at 1.0 mL.min −1 ).…”

“…The relative abundance (percent peak area) of three independent replicate samples of each compound identified by GC-MS without the use of correction factors is given in Table S1. Qualitative analysis was performed in the same way as described in Radulović and Blagojević (2010) and was based on (a) comparison of the mass spectra, (b) order of elution, and (c) co-injection of authentic standards (the corresponding pure compounds were added/spiked to cheese samples prior to HS-SPME). Standard (pure) compounds were obtained from Merck, Germany or Sigma-Aldrich, USA.…”

“…PCA and AHC were performed using the Excel program plug-in XLSTAT version 2011.3.02 and according to Radulović and Blagojević (2010). AHC was determined using Pearson dissimilarity where the aggregation criterion were simple linkage, unweighted pair-group average and complete linkage, and Euclidean distance where the aggregation criterion were weighted pair-group average, unweighted pair-group average, and Ward's method.…”