Plant Transposable Elements

Nevers, Patricia; Shepherd, Nancy S.; Saedler, Heinz

doi:10.1016/s0065-2296(08)60194-9

Cited by 192 publications

(81 citation statements)

References 243 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The isolation and characterization of maize transposable elements has led to the application of the cloned transposons as probes for the detection of mutated plant genes [6,11,25,26,27]. These transposon tagging experiments indicate that mutated plant genes are preferably isolated from lines which 1) carry mutations that confer a clearly distinguishable phenotype and 2) have a low copy number of the active transposable element in their genome.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

et al. 1989

View full text Add to dashboard Cite

Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Ac excises from the HPTII gene with comparable frequencies (30~o) in both plant species. When the /~-glucuronidase (GUS) gene was used to detect transposition of Tam3, a significantly lower excision frequency (13~o) was found in both plant species. It could be shown that deletion of parts of the transposable elements Tam3 and Ac, removing either one of the terminal inverted repeats (TIR) or part of the presumptive transposase coding region, abolished the excision from the marker genes. This demonstrates that excision of the transposable element Tam3 in heterologous plant species, as documented for the autonomous element Ac, also depends on both properties. Southern blot hybridization shows the expected excision pattern and the reintegration of Tam3 and Ac elements into the genome of tobacco plants.

show abstract

Section: Introductionmentioning

confidence: 99%

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

et al. 1989

View full text Add to dashboard Cite

show abstract

“…As the direction of mutation was from dominant green to recessive yellow, the mutant line represented an example of reverse variegation. This less common phenomenon has been earlier associated with transposable elements (Nevers et al, 1986). An example is the unstable mutation Six different variegated F2 plants of BOY 149 were selfed or backcrossed to LA780 using LA780 as male parent.…”

Section: Discussionmentioning

confidence: 86%

“…When cell-autonomous traits such as pigmentation or chlorophyll production are involved, somatic instability gives rise to the variegated patterns so often noticeable in flowers, kernels and leaves (Nevers et aL, 1986). In fact, variegation is one of the important features through which insertion mutations have been recognized.…”

Section: Introductionmentioning

confidence: 99%

“…In fact, variegation is one of the important features through which insertion mutations have been recognized. In addition to transposable elements, variegation has also been attributed to several other causes, including chimerism, virus infection, instability of (additional) chromosomal fragments, nutritional deficiencies (Kirk & Tilney-Bassett, 1978) and monogenic mutations (MartInez-Zapater et al, 1992 Nevers et al (1986) formulated a set of general rules to allow insertion mutations to be identified on the basis of genetic criteria. These include: (i) usually wildtype spots appear on a recessive background, indicating that the phenotypic switch is from recessive to dominant, (ii) segregation of the unstable character obeys the laws of Mendel, although stable recessives and wild-type revertants frequently arise as a result of the (im)precise excision of the element, (iii) new alleles are generated with heritable altered phenotypes, and (iv) in some instances a second factor is required for the instability.…”

Section: Introductionmentioning

confidence: 99%

“…Examples of the latter are to be found among the two-component systems such as Ac/ Ds and Spm/dSpm in Zea mays, where the autonomous element is capable of transactivating the non-autonomous element integrated at the locus responsible for the variegated phenotype. A number of specific mutants are known in which variegation is associated with transposable element insertions, although the phenotypic switch is from dominant to recessive (reverse variegation) (Nevers et a!., 1986). In some of these instances it has been shown that wild-type expression of genes carrying an insertion of a transposable element occurred, due to the splicing of the element from the transcript.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A re-investigation of the instability at the yv locus in tomato

Wisman

Ramanna

1994

Heredity

View full text Add to dashboard Cite

The instability at the yv locus on chromosome 6 of Lycopersicon esculentum has been re-investigated. Cytogenetic analysis of green, green-yellow variegated and yellow plants showed that the instability is not caused by the somatic segregation of extra chromosomal fragments as was proposed earlier by Hagemann. Instead, it is postulated that the variegated line carries an unstable recessive allele of yv (yvnw) that mutates frequently from dominant green to recessive yellow on the basis of the following observations: (i) variegated plants produced variegated and yellow offspring in aberrant ratios, and (ii) yellow male sterile plants appeared in populations derived from crosses of the Yvrns line with wild-type plants and the line LA 780, being recessive yv. Using the isozyme marker Aps-1 linked to yv, it was shown that the deficit in variegated and yellow mutants, which frequently occurs in F2 populations derived from crosses between the variegated mutant and wildtype yv plants, was due to certation. From the analysis of these F2 populations, it is concluded that the instability at yv is likely to be controlled autonomously. No evidence was obtained showing germinal reversion events to occur, as inferred from the absence of green plants among the selfed offspring. Variegated offspring were produced by a yellow plant which is interpreted to represent a somatic reversion event from yellow to variegated. Remarkably, when variegated plants were crossed to wild-type plants, F1 plants with a mutant phenotype appeared. On the basis of analogies with mutable alleles from maize and Drosophila, alternative mechanisms underlying the mutability of yv are discussed.

show abstract

Genetic Mosaics and Plant Improvement

Marcotrigiano

Gradziel

1997

Plant Breeding Reviews

View full text Add to dashboard Cite

Plant Transposable Elements

Cited by 192 publications

References 243 publications

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

A re-investigation of the instability at the yv locus in tomato

Genetic Mosaics and Plant Improvement

Contact Info

Product

Resources

About