Plant secondary metabolism linked glycosyltransferases: An update on expanding knowledge and scopes

Tiwari, Pragya; Sangwan, Rajender S.; Sangwan, Neelam S.

doi:10.1016/j.biotechadv.2016.03.006

Cited by 156 publications

(130 citation statements)

References 206 publications

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“…Two GST genes belonging to the phi and tau classes were upregulated 24 h after Fen induction. Another important gene superfamily, the glycosyl transferases, whose members can catalyze reactions of toxicants with sugars to form hydrophilic compounds ready for further catabolism, included four members that were also induced by the Fen treatments at 4 h [28].…”

Section: Functional Analysis Of the Differentially Expressed Genes (Dmentioning

confidence: 99%

Effects of fenclorim on rice physiology, gene transcription and pretilachlor detoxification ability

Yao

Cai

et al. 2020

BMC Plant Biol

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Background: Fenclorim (Fen) can effectively protect rice from pretilachlor (Pre) injury, but its effects on rice have not been formally evaluated; thus, the Fen mode of action for alleviating the phytotoxicity caused by Pre in rice is not clear. This study aimed to examine the biochemical and physiological effects of Fen on rice and to determine the changes induced by Fen at the transcriptome level.Result: The chlorophyll content of rice plants was significantly affected by Pre but not by Fen. The activity of oxidative stress enzymes showed that Fen did not elicit any changes in oxidative stress; however, it reduced lipid peroxidation and oxidative damage induced by Pre. Fen did not affect the uptake of Pre but did affect its persistence in rice. In a transcriptome experiment, Fen upregulated genes in a detoxification pathway. Overall, 25 genes related to detoxification were identified, including P450, GST, and GT. Moreover, qRT-PCR analysis showed that four P450 genes, CYP71Y83, CYP71K14, CYP734A2 and CYP71D55, and two GST genes, GSTU16 and GSTF5, were upregulated by Fen and/or Pre. Conclusion: Our work indicates that Fen acts in antioxidative defense in addition to enhancing the metabolism of herbicides in rice.

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Section: Functional Analysis Of the Differentially Expressed Genes (Dmentioning

confidence: 99%

Effects of fenclorim on rice physiology, gene transcription and pretilachlor detoxification ability

Yao

Cai

et al. 2020

BMC Plant Biol

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“…The family 1 UGTs involved in small molecule glycosylation are one of the largest of 103 glycosyltransferase families identified in bacteria, fungi, animals and plants (http://www.cazy.org/). UGTs glycosylate a variety of functional groups, including carboxyl and hydroxyl groups to produce O ‐glycosides, amine and sulphydryl groups to produce N ‐ and S ‐glycosides, respectively, and can also catalyse the formation of C ‐glycosides (Tiwari et al ., ). In plants, UGT sequences can be identified by the presence of the conserved plant secondary product glycosyltransferase (PSPG) motif which is involved in sugar donor binding and located in the C‐terminal region (Bowles et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Substrate specificity and safener inducibility of the plant UDP‐glucose‐dependent family 1 glycosyltransferase super‐family

Brazier-Hicks

Gershater

Dixon

et al. 2017

Plant Biotechnology Journal

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SummaryPlants contain large numbers of family 1 UDP‐glucose‐dependent glycosyltransferases (UGTs), including members that conjugate xenobiotics. Arabidopsis contains 107 UGT genes with 99 family members successfully expressed as glutathione transferase (GST)‐fusion proteins in E. coli. A high‐throughput catalytic screen was developed based on quantification of the fusion by measuring GST activity. UGT activity using UDP‐glucose as donor was then determined using 11 synthetic acceptors bearing hydroxyl, amino and thiol groups that had been shown to undergo conjugation in plant extracts. In total, 44 UGTs, largely members of the D and E groups, were active towards xenobiotics, glucosylating phenol and thiol acceptors. In contrast, N‐glucosyltransferase (NGT) activity was almost exclusively restricted to a single enzyme, UGT72B1. Using DNA microarrays, the induction of UGT transcripts following treatment with the herbicide safener fenclorim was compared in Arabidopsis and rice. D and L group members were the most safener‐inducible UGTs in both species. The respective Arabidopsis enzymes showed low conjugating activity towards xenobiotics. Using Genevestigator, a small group of safened D and L UGTs were consistently induced in response to biotic and abiotic stress suggestive of protective activities beyond xenobiotic detoxification in both species. The induction of other detoxifying gene families following treatment with fenclorim, namely cytochromes P450 and glutathione transferases, further confirmed the selective enhancement of related subfamily members in the two species giving new insight into the safening response in cereals, where herbicide tolerance is enhanced compared with dicots, which are unresponsive to these treatments.

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“…Glycosylation reactions are mediated by UDP-glycosyltransferases (UGTs) that catalyze the transfer of an activated nucleotide sugar to acceptor aglycones to form glycosides (Song et al, 2018). Recent studies have shown that UGTs also play an important role in plant growth and development and in response to biological and abiotic stresses (Tiwari et al, 2016;Rehman et al, 2018). To date, only a few volatiles and their corresponding UGTs have been functionally characterized due to the presence of hundreds of UGT-encoding genes in most plant species (Yonekura-Sakakibara & Hanada 2011;Caputi et al 2012;Song et al 2018) and substrate promiscuity of UGT enzymes (Jones & Vogt, 2001).…”

Section: Introductionmentioning

confidence: 99%

Sesquiterpene glucosylation mediated by glucosyltransferase UGT91Q2 is involved in the modulation of cold stress tolerance in tea plants

et al. 2020

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Summary Plants produce and emit terpenes, including sesquiterpenes, during growth and development, which serve different functions in plants. The sesquiterpene nerolidol has health‐promoting properties and adds a floral scent to plants. However, the glycosylation mechanism of nerolidol and its biological roles in plants remained unknown. Sesquiterpene UDP‐glucosyltransferases were selected by using metabolites‐genes correlation analysis, and its roles in response to cold stress were studied. We discovered the first plant UGT (UGT91Q2) in tea plant, whose expression is strongly induced by cold stress and which specifically catalyzes the glucosylation of nerolidol. The accumulation of nerolidol glucoside was consistent with the expression level of UGT91Q2 in response to cold stress, as well as in different tea cultivars. The reactive oxygen species (ROS) scavenging capacity of nerolidol glucoside was significantly higher than that of free nerolidol. Down‐regulation of UGT91Q2 resulted in reduced accumulation of nerolidol glucoside, ROS scavenging capacity and tea plant cold tolerance. Tea plants absorbed airborne nerolidol and converted it to its glucoside, subsequently enhancing tea plant cold stress tolerance. Nerolidol plays a role in response to cold stress as well as in triggering plant–plant communication in response to cold stress. Our findings reveal previously unidentified roles of volatiles in response to abiotic stress in plants.

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Plant secondary metabolism linked glycosyltransferases: An update on expanding knowledge and scopes

Cited by 156 publications

References 206 publications

Effects of fenclorim on rice physiology, gene transcription and pretilachlor detoxification ability

Effects of fenclorim on rice physiology, gene transcription and pretilachlor detoxification ability

Substrate specificity and safener inducibility of the plant UDP‐glucose‐dependent family 1 glycosyltransferase super‐family

Sesquiterpene glucosylation mediated by glucosyltransferase UGT91Q2 is involved in the modulation of cold stress tolerance in tea plants

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