Plant regeneration from leaf discs of peanut and pigeonpea: Influence of benzyladenine, indoleacetic acid and indoleacetic acid-amino acid conjugates

Eapen, Susan; George, L.

doi:10.1007/bf00037274

Cited by 61 publications

(38 citation statements)

References 15 publications

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“…Katsumi and Kazama (1978) reported enhanced auxin biosynthesis by supplementation of GA3, which may become occasionally toxic for growth and shoot elongation (Soetikno 1981). Eapen and George (1993) obtained regeneration of calli from the leaf discs of pigeon pea and peanut, and indicated that BAP + IAA or IAA + aspartic acid are essential for shoot bud elongation. In addition, Kumar et al (1983) reported regeneration from the leaf callus of pigeon pea on Blaydes (1966) medium with 0.5 mg L −1 BAP, 0.1 mg L −1 NAA, and 0.01 mg L −1 GA3.…”

Section: Shoot Bud Elongationmentioning

confidence: 99%

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea cv. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L −1 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observed on half-strength Murashige and Skoog (MS) medium with 2 % sucrose and 0.5 mg L −1 3-indolebutyric acid (IBA). On the other hand, the regeneration of globular embryos from cotyledon explant was faster and greater with thidiazuron (TDZ) than BAP with sucrose as carbohydrate source. These globular embryos were maturated on MS medium with abscisic acid (ABA) and finally germinated on half-strength MS medium at lower concentrations of BAP. Comparison of regeneration pathways in pigeon pea cv. JKR105 showed that the turnover of successful establishment of plants achieved through organogenesis was more compared to somatic embryogenesis, despite the production of more embryos than shoot buds.

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Section: Shoot Bud Elongationmentioning

confidence: 99%

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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“…BAP and GA 3 each 0.5 mg l −1 showed optimum elongation of the regenerated shoots. Earlier workers reported the successful elongation of regenerated shoots with application of BAP and IAA together (Eapen and George 1993). However, GA 3 alone or in combination with BAP and IAA also elongated micro-shoots (Dayal et al 2003;Eapen et al 1998;Villiers et al 2008).…”

Section: Discussionmentioning

confidence: 97%

In vitro shoot grafting on rootstock: An effective tool for <i>Agrobacterium</i>-mediated transformation of pigeonpea (<i>Cajanus cajan</i> (L.) Millsp.)

Ghosh

Purohit

Ganguly

et al. 2014

Plant Biotechnology

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Lack of reproducible in vitro transformation method in pigeonpea limits the application of biotechnological breeding approaches for its genetic improvement. The present study describes a transformation method using novel in vitro shoot grafting technique for two cultivars ICPL87 and ICPL87119. Modified Murashige and Skoog (MS) medium with 1 mg l −1 6-benzylaminopurine and 0.2 mg l −1 α-naphthaleneacetic acid induced an average of 25 shoots from decapitated embryonic axis explants after six weeks of culture. These shoots were further elongated in a modified MS medium containing 0.5 mg l −1 6-benzylaminopurine along with 0.5 mg l −1 gibberellic acid for another four weeks. Grafting of pigeonpea shoots to seedling rootstock allowed 95% recovery of shoots. The whole regeneration process, starting from explant preparation to complete plant development, took 12-13 weeks. Further, the explants were infected with Agrobacterium tumefaciens harboring a binary vector pBI121. Transient and constitutive β-glucuronidase expressions were obtained in putative transgenic shoots selected at 100 mg l −1 kanamycin. The selected shoots were grafted on non-transgenic root stock to establish putative transformants. T 0 and T 1 transformants were confirmed through polymerase chain reaction for presence of neomycin phosphotransferase gene. An overall 9% of transformation efficiency was recorded in both cultivars.

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“…Another effect of amino acids in the tissue culture is the synergic effect with the plant growth regulators, as found in pigeonpea and peanut. In the peanut, the highest frequency of plant regeneration was induced by the BAP in combination with IAA or IAA-lalanine, while in pigeonpea, the BAP in combination with IAA or IAA-l-aspartic acid produced best results (Eapen and George 1993).…”

Section: Amino Acidsmentioning

confidence: 95%

Fox grape cv. Bordô (Vitis labrusca L.) and grapevine cv. Chardonnay (Vitis vinifera L.) cultivated in vitro under different carbohydrates, amino acids and 6-Benzylaminopurine levels

Carvalho

Silva

Schuck

et al. 2013

Braz. arch. biol. technol.

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Plant regeneration from leaf discs of peanut and pigeonpea: Influence of benzyladenine, indoleacetic acid and indoleacetic acid-amino acid conjugates

Cited by 61 publications

References 15 publications

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

In vitro shoot grafting on rootstock: An effective tool for <i>Agrobacterium</i>-mediated transformation of pigeonpea (<i>Cajanus cajan</i> (L.) Millsp.)

Fox grape cv. Bordô (Vitis labrusca L.) and grapevine cv. Chardonnay (Vitis vinifera L.) cultivated in vitro under different carbohydrates, amino acids and 6-Benzylaminopurine levels

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