Plant regeneration from different explants in Helianthus smithii Heiser

Laparra, Hélène; Стоева, П.; Ivanov, P.; Hahne, Günther

doi:10.1007/s002990050303

Cited by 17 publications

(21 citation statements)

References 8 publications

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“…This pattern of BA impact was also observed on other species of Asteraceae [28,37,40]. Previous reports show that somatic embryos or adventitious shoots could be proliferated directly from explants using only 2,4-D, BA, or NAA + BA [40,[44][45][46]. This different response to PGR may have some relation to the endogenous growth regulator levels in the explants.…”

Section: Discussionmentioning

confidence: 68%

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

Kang

Sheng

et al. 2012

Acta Soc Bot Pol

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Goldenrod (Solidago canadensis L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog’s medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological variation when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed

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Section: Discussionmentioning

confidence: 68%

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

Kang

Sheng

et al. 2012

Acta Soc Bot Pol

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“…Media containing benzyladenine (BA) (2.22-8.87 µM) together with NAA (0.27-0.54 µM) were preferably used for multiple shoot induction and maintenance in Asteraceae plants (Winand et al 1986;Akita et al 1994;Laparra et al 1997;Stojakowska and Kisiel 1997;Wildi et al 1998;Liu et al 1998). However, our preliminary attempts to induce axillary shoot proliferation using MS medium containing 0.25 µM NAA together with 0.25-10.0 µM BA failed either due to low multiplication rate (up to 1.8 ± 0.5 using 2.5 µM BA) or due to abundant callus formation using 5.0--10.0 µM BA.…”

Section: Resultsmentioning

confidence: 99%

In vitro propagation of lnula royleana DC

Stojakowska

Malarz

2011

Acta Soc Bot Pol

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A micropropagation method, through axillary shoot proliferation, was elaborated for Inula royleana DC. (Asteraceae), a medicinal plant native of Himalaya. Primary explants (cotyledonary node explants) and secondary explants (node explants of in vitro regenerated shoots) of the plant, inoculated on MS medium supplemented with 0.1 µM NAA and 5.0 µM kinetin, regenerated 3.4 ± 1.2 and 5.1 ± 1.9 axillary shoots per explant, respectively. The regenerated shoots were easily rooting and adapting to growth in soil.

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“…World sunflower production has increased by 70% over the past decade (Alibert et al, 1994). Several methods for sunflower regeneration have been reported (reviewed by Alibert et al, 1994), including organogenesis (Knittel et al, 1991;Pugliesi et al, 1991;Ceriani et al, 1992;Chraibi et al, 1992); somatic embryogenesis (Pelissier et al, 1990;Jeannin and Hahne, 1991;Fiore et al, 1997;Laparra et al, 1997); and shoot proliferation from meristematic tissue. Most recent studies on sunflower regeneration by direct organogenesis report a dependency on both culture conditions (Chraibi et al, 1992) and genotype (Samaj et al, 1994;Sarrafi et al, 1996;Fiore et al, 1997).…”

Section: Introductionmentioning

confidence: 97%

A shoot regeneration protocol effective on diverse genotypes of sunflower (Helianthus annuus L.)

Shin

Kim

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

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We describe a protocol, and several experiments that helped lead to its development, for sunflower regeneration. Important factors for sunflower regeneration were explant age, cytokinin type and concentration, basal medium, and explant source. We could not induce shoot regeneration from the explants derived from mature tissues including leaf, petiole, and stem. However, use of juvenile explants such as embryo meristem and primordial leaf tissues allowed routine regeneration of 17 different sunflower genotypes. High frequency of shoot regeneration was achieved with these explants taken from seedlings up to 5 d after germination. Explant age was less critical for embryo meristem explants than for primordial leaf tissues. Of the four basal media tested, MS and B5 media produced higher shoot-regeneration frequencies than did Anderson and woody plant media. The highest shoot-regeneration frequency was obtained with MS medium supplemented with 2 mM BA and without auxin. Addition of 1 mM naphthalene-acetic acid to the medium significantly reduced both the percentage of explants producing shoots and average number of shoots per explant. Regenerated shoots were grown to maturity in a greenhouse.

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Plant regeneration from different explants in Helianthus smithii Heiser

Cited by 17 publications

References 8 publications

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

In vitro propagation of lnula royleana DC

A shoot regeneration protocol effective on diverse genotypes of sunflower (Helianthus annuus L.)

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