In vitro propagation of lnula royleana DC

Stojakowska, Anna; Malarz, Janusz

doi:10.5586/asbp.2004.001

Cited by 10 publications

(11 citation statements)

References 15 publications

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“…In both vegetables, leaf explants did not respond well to growth regulators. BA alone or in combination with Indole Acetic Acid (IAA) or NAA is used for in vitro propagation and multiple shoot regeneration of other species (Avani et al, 2006;Sakpere et al, 2011;Sharma et al, 2009;Stojakowska and Malarz, 2004). This is the first report of vegetative propagation of C. volubile in which tip and softwood cuttings were found to be best for vegetative propagation.…”

Section: Discussionmentioning

confidence: 86%

Investigation of Nutritional Composition and Vegetative Propagation of Two Leafy Vegetables

Sakpere

Olaitan

Alonge

2013

International Journal of Vegetable Science

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There is little known of the nutritional content of underutilized leafy vegetables and the efficacy of vegetative propagation of these plants. The nutrient content of Solanecio biafrae (Oliv. & Hiern) C. Jeffrey and Clerodendrum volubile P. Beauv. was measured, and the efficacy for mass vegetative propagation was determined. Protein, fat, ash, crude fiber, and carbohydrate contents of S. biafrae were either the same as or lower than previously reported. Protein, fat, ash, and crude fiber of C. volubile were lower than previously reported. The requirement for vitamin C can be met by consuming 400 g of S. biafrae or 200 g of C. volubile per day. The K and Ca contents in S. biafrae were lower and higher, respectively, than previously reported, and other elements, including Fe, were lower than previously reported. Elemental composition of C. volubile was either the same as, lower, or higher than previously reported. Tip and softwood cuttings of C. volubile were best for vegetative propagation and S. biafrae can be propagated vegetatively using two to three nodal cuttings. Both vegetables can be used for in vitro regeneration.

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Section: Discussionmentioning

confidence: 86%

Investigation of Nutritional Composition and Vegetative Propagation of Two Leafy Vegetables

Sakpere

Olaitan

Alonge

2013

International Journal of Vegetable Science

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“…Leaf explants that had generated roots on medium supplemented with 0.1 mg/l 2,4-D sub-cultured unto a medium supplemented with 0.1 mg/l 2,4-D and 1.0 mg/l Kinetin generated callus while leaf explants inoculated on medium supplemented with 0.5 mg/l Kinetin and 0.1 mg/l 2,4-D, generated compact, dark brown callus after which shoots emerged from the explant followed by more roots (Plate 1B). According to Stojakowska and Malarz (2004), the tendency to indirect organogenesis was diminished when Kinetin was used in cultures.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Media containing Benzyl adenine (BA) together with NAA have been reported to be preferably used for multiple shoot induction and maintenance in Asteraceae plants (Stojakowska and Malarz, 2004). According to Trejgell et This needs to be tested for Launea taraxacifolia since it is known that different species can respond differently to the same growth regulators.…”

Section: Plant Materialsmentioning

confidence: 99%

Potential of Launea taraxacifolia (Willd) Amin Ex. C. Jeffrey for in Vitro Regeneration

2011

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This study investigated the potential of Launea taraxacifolia for in vitro regeneration. Stem and leaf explants were inoculated on full strength Murashige and Skoog (MS) medium supplemented with varying concentrations of 2, 4-dichlorophenoxyacetic acid (2,4-D). Leaf explants responded to all concentrations of 2,4-D used while stem explants responded to only two of the 2, 4-D concentrations suggesting that leaf explants might be a better source of explants. Leaf explants generated shoots on medium supplemented with 0.5 mg/l kinetin and 0.1 mg/l 2, 4-D. This study is the first report on in vitro regeneration of Launea taraxacifolia.

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“…Jabeen et al (2007) reported that the presence of auxin was necessary for roots induction on I. racemosa shoots and IBA was the most effective for this species. Stojakowska and Malarz (2004) used 0.02 mg l -1 IBA, which accelerated the rooting process, but had no effect on percentage of I. royleana rooted shoots. Moreover, the shoots of I. verbascifolia were rooted on MS medium supplied with 0.2 mg l -1 IBA (Thiem et al, 2003).…”

Section: Rooting and Acclimatizationmentioning

confidence: 99%

“…The species of Inula genus, I. viscose (Romano, 1997), I. royleana (Stojakowska and Malarz, 2004), I. verbascifolia transferred into plastic pots filled with a sterile mixture of vermiculite and sand (1:1). The plantlets from liquid medium were directly transferred to pots filled with the same substrate.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Inula germanica L. from the Seedlings Explants

Trejgell

Kamińska

Lisowska

et al. 2018

Not Bot Horti Agrobo

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This is the first communication of micropropagation system for Inula germanica using seedling explants germinated in vitro. The development of this system gives the possibility of future reintroduction of I. germanica providing a way to stabilize or re-establish its population. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from ten-day-old seedlings. Explants were put on MS medium containing 1.0 mg l ) at 26 ± 1 °C. The highest percentage of shoot organogenesis (83.3%) was recorded for hypocotyl, while the highest average number of shoots per explant (12.0) was recorded for shoot tips. In subsequent subcultures, multiplication rate decreased to 3.0-4.9 shoots per explant. Less than 19% shoots were able to root on the solid medium without auxins. The highest rooting efficiency (69.3%) was recorded for solid medium supplemented with indolebutyric acid, but growth of roots was inhibited. The percentage of rooted shoots (62.2%) and number of roots per shoot (2.4 per shoot) into the liquid medium were comparable to medium with 0.1 mg·l -1 indolebutyric acid. showing a positive impact on the process of acclimatization. The regenerated plants were able to flowering in the first year after acclimatization. Developed micropropagation system for I. germanica is efficient and can be a useful tool for the active protection of this species.

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In vitro propagation of lnula royleana DC

Cited by 10 publications

References 15 publications

Investigation of Nutritional Composition and Vegetative Propagation of Two Leafy Vegetables

Investigation of Nutritional Composition and Vegetative Propagation of Two Leafy Vegetables

Potential of Launea taraxacifolia (Willd) Amin Ex. C. Jeffrey for in Vitro Regeneration

Micropropagation of Inula germanica L. from the Seedlings Explants

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