Plant Regeneration from Cultured Immature Embryos and Inflorescences of 25 Cultivars of Wheat (<i>Triticum aestivum</i>)

Maddock, S. E.; Lancaster, Vicki; Risiott, R.; Franklin, J. F.

doi:10.1093/jxb/34.7.915

Cited by 200 publications

(99 citation statements)

References 25 publications

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“…For induction response, results in the literature range from 92-100% (Ozgen et al 1996), 20-100% (Machii et al 1998) and for regeneration capacity, from 29 -100% (Maddock et al 1983); 0-60% (Mathias and Simpson 1986); 0-90% (Machii et al 1998); and 28-60% (Li et al 2003).…”

Section: Resultsmentioning

confidence: 99%

Callus induction and plant regeneration by Brazilian new elite wheat genotypes

Vendruscolo¹,

Schuster²,

Negra³

et al. 2008

CBAB

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-The distinction of genotypes responsive to tissue culture and the development of an efficient regeneration system are the first steps towards transgenic plant production. Nine Brazilian wheat (Triticum aestivum L.) genotypes were cultivated in vitro to evaluate the embryogenetic capacity. The explants (immature zygotic embryos) were tested in two different culture media, MS (Murashige and Skoog 1962) and modified MS -MMS (Zhou et al. 1995)

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Section: Resultsmentioning

confidence: 99%

Callus induction and plant regeneration by Brazilian new elite wheat genotypes

Vendruscolo¹,

Schuster²,

Negra³

et al. 2008

CBAB

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“…For other monocots such as barley (Hordeum vulgare), wheat, rice, and sorghum, immature embryos remain the predominant transformation target, despite reports over the years of successfully initiating tissue culture responses from explants other than immature embryos. These alternative explants have included (1) leaf bases to initiate callus cultures in maize (Wenzler and Meins, 1986), rice (Ramesh et al, 2009), and wheat (Yu et al, 2012); (2) immature inflorescences to initiate cultures in sorghum (Brettell et al, 1980), wheat (Maddock et al, 1982;OziasAkins and Vasil, 1982), rice (Chen et al, 1985;Rout and Lucas, 1996), barley (Wen et al, 1991), tritordeum (Barcelo et al, 1994), and maize (Pareddy and Petolino, 1990); (3) multiple-shoot cultures from apical meristems in maize (Zhong et al, 1992); and (4) regenerable callus from mature seed-derived embryos in rice (Lee et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

et al. 2016

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While transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of most academic laboratories. Here, we report a transformation approach involving overexpression of the maize (Zea mays) Baby boom (Bbm) and maize Wuschel2 (Wus2) genes, which produced high transformation frequencies in numerous previously nontransformable maize inbred lines. For example, the Pioneer inbred PHH5G is recalcitrant to biolistic and Agrobacterium tumefaciens transformation. However, when Bbm and Wus2 were expressed, transgenic calli were recovered from over 40% of the starting explants, with most producing healthy, fertile plants. Another limitation for many monocots is the intensive labor and greenhouse space required to supply immature embryos for transformation. This problem could be alleviated using alternative target tissues that could be supplied consistently with automated preparation. As a major step toward this objective, we transformed Bbm and Wus2 directly into either embryo slices from mature seed or leaf segments from seedlings in a variety of Pioneer inbred lines, routinely recovering healthy, fertile T0 plants. Finally, we demonstrated that the maize Bbm and Wus2 genes stimulate transformation in sorghum (Sorghum bicolor) immature embryos, sugarcane (Saccharum officinarum) callus, and indica rice (Oryza sativa ssp indica) callus.

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“…Creso and T. These were grown to maturity in a controlled environment room(16-h photoperiod of 275 1uE m2 s' provided by 125 W white fluorescent tubes and 25 W tungsten bulbs; day: 20°C night: 16°C,). Callus was initiated from immature embryos according to Maddock et al, 1983, although with 2 mg 1 1 2,4-D and no coconut milk. After 28 days, all the callus was transferred into liquid MS (Murashige & Skoog, 1962) medium containing S mg 1 2,4-D. Suspensions were subcultured every 7 days.…”

Section: Cell Suspensionsmentioning

confidence: 99%

A comparison of chromosome instability in cell suspensions of diploid, tetraploid and hexaploid wheats

1993

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Chromosome instability was monitored' through time in cell suspensions of the diploids Triticum tauschii (the D genome donor) and T monococcum (the A genome donor), the tetraploids, T durum and T dicoccum (both AABB) and in the cultivars Copain and Sicco of hexaploid (AABBDD) breadwheat (T aestivum). Differences in stability were observed between the different ploidy levels and between species within the ploidy levels. The diploids were most stable, particularly T. tauschii, and the hexaploids were least stable. Instability was greatest in all the lines during the first months of culture. In all but one line, very few structural aberrations were observed, even after many months in culture. Morphogenetic capacity was lost in all lines, even though some retained high proportions of euploid cells. The relevance of these results is discussed in relation to the regeneration of breadwheat from protoplasts.

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Plant Regeneration from Cultured Immature Embryos and Inflorescences of 25 Cultivars of Wheat (Triticum aestivum)

Cited by 200 publications

References 25 publications

Callus induction and plant regeneration by Brazilian new elite wheat genotypes

Callus induction and plant regeneration by Brazilian new elite wheat genotypes

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

A comparison of chromosome instability in cell suspensions of diploid, tetraploid and hexaploid wheats

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