Plant Regeneration from Cucumber Protoplasts

Colijn-Hooymans, C. M.; Bouwer, R.; Dons, J.J.M.

doi:10.1007/978-94-009-2788-9_30

Cited by 10 publications

(5 citation statements)

References 3 publications

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“…Since Cu is also a component of the Cu-Zn Superoxide dismutase, it is directly involved in the mechanism of detoxification of O 2 generated in photosynthesis (Elstner, 1982). In this context, a significant promotive effect of light intensity on callus induction and somatic embryogenesis has been previously reported for a number of vegetable and ornamental species, such as cucumber (Cucumis sativus) Downloaded by [North West University] (Cade et al, 1988;Colijn-Hooymans et al, 1988), melon (Cucumis meld) (Gray et al, 1993;Kintzlos et al, 1997), pepper (Capsicum annuum) and rose (Rosa hybrids) (Kintzlos et al, 1998). Experiments with common mallow (Malva sylvestris) tissue cultures have also demonstrated that higher light intensities are favorable for somatic embryo proliferation (Kintzios, unpublished data).…”

Section: Effects Of Inorganic Micronutrients On Callus Growth and Sommentioning

confidence: 95%

Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from young mature leaves of rose

Kintzios

Drossopoulos

Lymperopoulos

2000

Journal of Plant Nutrition

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The effect of different vitamins and inorganic micronutrients on callus growth and on the induction and proliferation of somatic embryos from young mature, fully expanded leaves of rose (Rosa hybrids L.) was investigated. Expiants were cultured onto a solid Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose, 53.5 µM p-chlorophenoxyacetic acid, 4.6 µM Kinetin, and 0.5 mg L -1 thiamin.HCI in combination with either of eleven different vitamins. Alternatively, expiants were cultured onto a solid medium containing MS macro-and micronutrients except for the salts of manganese (Mn), zinc (Zn), iodine (I), copper (Cu), and cobalt (Co), which were added at either the standard MS concentration or at a tenfold increased (Cu, Co) or decreased (Mn, Zn, I) concentration. Embryo maturation and germination took place on a MS medium supplemented with 5.2 µM 6-benzyladenine and 5.7 µM 3-indole-acetic acid. The results indicated that somatic embryogenesis 1 Corresponding author.

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Section: Effects Of Inorganic Micronutrients On Callus Growth and Sommentioning

confidence: 95%

Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from young mature leaves of rose

Kintzios

Drossopoulos

Lymperopoulos

2000

Journal of Plant Nutrition

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“…2A). Callused explants generally formed several (3)(4)(5)(6)(7)(8) immature embryos or shoot primordia. Mature somatic embryos were obtained (Fig.…”

Section: Mode Of Regeneration In C Sativus and Influence Of Growth Rmentioning

confidence: 99%

“…Average frequencies of regeneration have ranged from 5% to 37%, and explants tested include cotyledons [3,11,18,19], roots [18], hypocotyls [2,8,16,19] and leaves [12]. Regeneration has also been accomplished from protoplasts [5,10,18] and from suspension cultures [4]. Regeneration of the closely related sexually compatible C. sativus var.…”

Section: Introductionmentioning

confidence: 99%

Regeneration of Cucumis sativus var. sativus and C. sativus var. hardwickii, C. melo, and C. metuliferus from explants through somatic embryogenesis and organogenesis

Punja

Abbas

Sarmento

et al. 1990

Plant Cell Tiss Organ Cult

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Regeneration of Cucumis sativus var. sativus and C. sativus var. hardwickii, C. melo, and C. metuliferus from explants through somatic embryogenesis and organogenesis Influence of explant source, growth regulator regime and genotype Abstract Regeneration in six inbred lines or F~ hybrids of Cucumis sativus was achieved on Murashige & Skoog's medium containing various concentrations of 2,4-D/BA, NAA/BA, NAA/Z or NAA/K. The range of regeneration frequency for cotyledon, leaf and petiole explants was 0-38, 0-75 and 14-96%, respectively, after 6-8 weeks in culture. Only one subculture of calli to growth regulator-free medium was required for regeneration. Preincubation of explants in the dark for 2-3 weeks was essential to achieve optimal regeneration. Highest frequency of plantlet formation occurred with petiole explants incubated on NAA/BA (5.0/2.5 #M), NAA/Z (5.0/5.0#M) or 2,4-D/BA (5.0/5.0/~M). Approximately 80% of these plantlets survived after transplanting to greenhouse soil, and they flowered and set fruit. The F~ hybrid, Endeavor, gave the highest regeneration frequency of 91% on 2,4-D/BA at 5.0/5.0 #M. Formation of somatic embryos was observed on 2,4-D/BA, while organogenesis and embryogenesis both were evident on NAA/BA and NAA/Z. Cotyledonary explants yielded the lowest frequency (ca. 7%) of plantlet formation in this study. Plantlets of C. sativus var. hardwickii and an F~ hybrid of C. sativus x C. s. var hardwickii were regenerated on NAA/Z and NAA/K at frequencies of 15-65 %, predominantly by the formation of somatic embryos. Shoots were obtained from cotyledon and leaf explants of C. me'tuliferus on IAA/BA (7.5•5.0 ~tM) and from leaf and petiole explants of C. melo on NAA/BA (5.0/2.5/~M), but plantlets were recovered only in C. melo.Abbreviations: BA-benzyladenine; 2,4-D-2,4-dichlorophenoxy-acetic acid; IAA-indoleacetic acid; Kkinetin; MS-Murashige & Skoog's medium; NAA-naphthaleneacetic acid; Z-zeatindihydroside ethanol for 20s, soaked for 10-12min in 7.5% commercial bleach, and rinsed three times in sterile distilled water prior to use.

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“…Many researchers noted that the incubation of cell cultures under a reduced density of photosynthetic photon flux or in the dark would be preferable for the induction of somatic embryos in many species, such as melon (Gray et al 1993;Guis et al 1997;Kintzios and Taravira 1997), cucumber (Cade et al 1988;Colijn-Hooymans et al 1988), and chili pepper (Kintzios et al 2000); but as far as induction of shoots (organogenesis), it was demonstrated that in melon light is necessary for the regeneration of cotyledons explants (Leshem et al 1995).…”

Section: Effect Of Darkness On Regeneration and Ploidy Levelmentioning

confidence: 99%

Adventitious regeneration from haploid melon (Cucumis melo L.) leaves as an approach to increase the frequency of diploid plants

Mahdad

Menéndez

Claveria

et al. 2023

In Vitro Cell.Dev.Biol.-Plant

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Plant Regeneration from Cucumber Protoplasts

Cited by 10 publications

References 3 publications

Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from young mature leaves of rose

Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from young mature leaves of rose

Regeneration of Cucumis sativus var. sativus and C. sativus var. hardwickii, C. melo, and C. metuliferus from explants through somatic embryogenesis and organogenesis

Adventitious regeneration from haploid melon (Cucumis melo L.) leaves as an approach to increase the frequency of diploid plants

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