Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from young mature leaves of rose

Kintzios, Spyridon; Drossopoulos, J. B.; Lymperopoulos, Ch.

doi:10.1080/01904160009382111

Cited by 27 publications

(34 citation statements)

References 28 publications

(20 reference statements)

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“…Cotyledons placed with the adaxial side of the lamina onto the medium and the fragments of hypocotyls produced the lowest number of adventitious leaves, and in the case of line SF9 no organogenesis was observed on these explants when glycine was present in the medium. Kintzios et al (2001) claimed that the elimination of glycine from the vitamins in MS medium is advantageous for the effectiveness of the callogenesis and somatic embryogenesis in pepper. The results of these studies indicate that the lack of glycine in the medium also favors direct organogenesis in tested sweet and pungent cultivars.…”

Section: Resultsmentioning

confidence: 99%

“…The effectiveness of the 4 factors recommended for that stage of organogenesis was estimated for all the investigated cultivars. We added 1.1 mg L -1 IAA (Joshi and Kothari, 2007) for medium Ua, 0.5 mg L -1 indole-3-butyric acid (IBA) (Sanatombi and Sharma, 2007) for medium Ub, and 3 mg L -1 BA and 2 mg L -1 2,4-dichlorophenoxyacetic acid (2,4-D) (Kintzios et al, 2001) for medium Uc, as well as using a 50% dilution of MS medium (except for agar) (Kumar et al, 2007) for medium ½MS. The effectiveness of rooting was also verified after applying the method of pouring auxin on the growth regulator-free medium.…”

Section: Rooting and Growthmentioning

confidence: 99%

“…of agar was used in the experiments. The composition of vitamins based on the research of Kintzios et al (2001) included 100.0 mg L -1 meso-inositol, 0.5 mg L -1 thiamine HCl, 0.5 mg L -1 pyridoxine HCl, and 0.1 mg L -1 nicotinic acid. A research factor, 2.0 mg L -1 glycine, was added (medium a 1 ) or its presence was omitted (medium a 2 ) in the media.…”

Section: Sterilization and Initial Explants Preparationmentioning

confidence: 99%

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In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types

Orlinska¹,

Nowaczyk²

2015

Turk J Biol

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This study evaluates the effectiveness of in vitro regeneration of 4 genotypes of pepper (Capsicum spp.) that differ in origin and functional properties: California Wonder, (ATZ × Sono)F 1 , Jalapeno, and SF-9. In order to investigate the effect of the initial explant type, photoperiod, and presence of 2.0 mg L -1 of glycine in the medium, organogenesis was induced on the MS medium with 2.0 g L -1 2-(N-morpholine) ethanesulfonic acid, 1.7 mg L -1 AgNO 3 , 0.4 mg L -1 indole-3-acetic acid (IAA), and 6.0 mg L -1 6-benzyladenine. The effectiveness of organogenesis in the 3 genotypes representing C. annuum L. was similar and considerably lower for line SF9 derived from an interspecific hybrid (C. frutescens L. × C. annuum L.). The highest number of adventitious leaves was recorded in the decapitated seedlings exposed to continuous light. The lack of glycine in the medium favored direct organogenesis. A simultaneous formation of adventitious leaves with a stable number of chromosomes and mixoploid callus tissue was determined by flow cytometry. The successive stage of foliaged shoots and roots formation was most effective in the MS medium with 1.1 mg L -1 IAA. The use of these optimal conditions resulted in developing 42%-56% of properly formed diploid plantlets, successfully acclimatized in 86%-97%.

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Section: Resultsmentioning

confidence: 99%

Section: Rooting and Growthmentioning

confidence: 99%

Section: Sterilization and Initial Explants Preparationmentioning

confidence: 99%

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In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types

Orlinska¹,

Nowaczyk²

2015

Turk J Biol

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“…O mesmo foi observado para a amoreira-preta, onde na ausência de inositol ocorreu a formação de calos na base das plântulas. Kintzios et al (2000Kintzios et al ( , 2001 observaram em um estudo com folhas de rosa (Rosa hybrida) e de Capsicum annuum que, na presença de 100 mg L -1 de inositol um maior crescimento de calos foi obtido. Esses verificaram também que a glicina a 0,1 mg L -1 foi a menos favorável para o mesmo crescimento e proliferação de embriões somáticos de C. annuum.…”

Section: Introductionunclassified

Efeito de concentrações de glicina e inositol no cultivo in vitro de duas frutíferas de clima temperado

et al. 2008

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RESUMOA fim de aprimorar técnicas de cultivo in vitro de duas frutíferas de clima temperado (amoreira-preta cv. Tupy e porta-enxerto de videira cv. Kobber 5BB), testaram-se diferentes concentrações de glicina e inositol, adicionadas ao meio de cultura. Para plântula de amoreirapreta, o meio foi constituído do meio básico MS, acrescido de 30 g L -1 de sacarose e 7 g L -1 de ágar, e o pH ajustado para 5,8 antes da autoclavagem a 121 º C e 1 atm por 20 minutos; e do meio básico DSD1 para porta-enxerto de videira cv. Kobber 5BB, acrescido de 20 g L -1 de sacarose e 7 g L -1 de ágar, e o pH ajustado para 6,4. O experimento com amoreira-preta consistiu de 5 diferentes concentrações de glicina (0; 1,0; 2,0; 4,0 e 8,0 mg L ) e 4 vezes o valor de inositol. Para o porta-enxerto de videira, melhores resultados foram obtidos na ausência e/ou com baixas concentrações de glicina e concentração de inositol igual ou superior à recomendada no meio de cultura DSD1.Termos para Indexação: Vitamina, aminoácido, MS, DSD1, amoreira-preta, videira. ABSTRACTAiming to improve the in vitro cultivation techniques of two temperate fruit i.e. blackberry cv. Tupy and grapevine rootstock cv. Kobber 5BB, different glycine and inositol concentrations in the culture medium were tested. The culture medium was constituted of MS basal medium, added of 30 g L -1 sucrose and 7 g L -1 agar, and the pH adjusted to 5.8 before the sterilization of 121ºC and 1 atm for 20 minutes, and DSD1 basal medium for grapevine rootstock cv. Kobber 5BB, added of 20 g L -1 sucrose and 7 g L -1 agar, and the pH adjusted to 6. ) and 4 fold of the inositol value. For the grapevine rootstock, better results were obtained in the absence and/or with low glycine concentrations and the same or higher inositol concentration as recommended in the DSD1 culture medium.

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“…The elimination of Zn 2+ stimulated somatic embryogenesis in wheat (He et al 1991). However, a tenfold decrease of its concentration (3 μM) did not affect somatic embryogenesis in chilli pepper (Kintzios et al 2001). In barley, an increase of Zn 2+ from 30 to 52 μM resulted in an enhanced callus formation in leaf segments (Pasternak et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Zinc sulphate improved microspore embryogenesis in barley

Echávarri

Soriano

Cistué

et al. 2008

Plant Cell Tiss Organ Cult

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Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from young mature leaves of rose

Cited by 27 publications

References 28 publications

In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types

In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types

Efeito de concentrações de glicina e inositol no cultivo in vitro de duas frutíferas de clima temperado

Zinc sulphate improved microspore embryogenesis in barley

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