Plant Regeneration from Citrus Root Meristems

Factors affecting the establishment and maintenance of ?Titan? red raspberry root organ cultures

“…This was achieved via an intervening stage of in vitro shoot tip culture. In most other instances reported in the literature, woody plant root cultures have been started from excised seedling radicles [8,14,16,19,20,21,23,25], presumably because roots from adult plants grown in soil are very difficult to successfully surface sterilize and hence difficult to establish in axenic culture. Furthermore, seedling roots would exhibit greater growth rate and a greater potential for organogenesis than adult phase explants [9].…”

“…Formation of lateral roots by root cultures of Acer rubrum was unaffected by auxin [3]. l~nus roots also form laterals in culture without auxin but the process is greatly stimulated by auxin [4,19,23,25] : Root cultures of two species of Otrus [20] did not form laterals at all, even in the presence of the auxin, 2,4-D.…”

“…At least 12 species of herbaceous plants have been regenerated from root cultures via adventitious bud formation [12,18,27 and references cited therein]. On the other hand, plantlet regeneration from root cultures has succeeded only with 6 woody species, including Comptonia peregrina [8], Robinia pseudoacacia [21], Citrus sinensis, Citrus limon [20] and Dalbergia sissoo [16], and Actinidia chinensis [10]. A natural tendency to reproduce in soil by root suckering (formation of adventitious buds on an intact root system) and the use of juvenile explants (excised radicles from asceptically germinated seeds) in *To whom all correspondence should be sent.…”

Morphogenesis and tissue cultures of three citrus species

“…Morphogenesis of citrus tissue cultures has been described from ovules and nucelli [16], seeds [14], stem segments [3,10] and somatic callus derived from leaf, stem and root segments [5,6,7]. These studies covered a wide range of citrus species and were aimed at the recovery of virus-free plants or the definition of high-efficiency micropropagation protocols.…”

Estudo de fatores que influenciam o processo de transformação genética em citros via Agrobacterium tumefaciens.

“…A regeneração via organogênese em Citrus, tem sido descrita para vários tecidos incluindo raízes (Bhat et al, 1992;Sauton et al, 1982;Sim et al, 1989;Edriss & Burger, 1984;Goh et al, 1995), discos de folha (Chaturvedi & Mitra, 1974), segmento internodal (Barlass & Skene, 1982;Durán-Vila et al, 1989;Moore, 1986), segmento de cotilédone (Sim et al, 1989;Goh et al, 1995), segmento de epicótilo (Sim et al, 1989;Edriss & Burger, 1984;Goh et al, 1995;Magon & Singh, 1995;García-Luis et al, 1999;Moreira-Dias et al, 2000) ou de hipocótilo (Magon & Singh, 1995).…”