Plant regeneration from callus and suspension cultures of Valeriana edulis ssp. procera via simultaneous organogenesis and somatic embryogenesis

Castillo, Patricia Negreros; Márquez, Judith; Rubluo, A.; Hernández, Georgina; Lara, Miguel

doi:10.1016/s0168-9452(99)00203-4

Cited by 36 publications

(27 citation statements)

References 12 publications

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“…A similar result was observed in Pelargonium, where the organogenic callus induction was mediated by high cytokinin and low auxin (Saxena et al 2000). Callus induction through an auxin-cytokinin combination has been reported for several systems, including Astragalus adsurgens (Luo et al 1999), Lavandula (Dronne et al 1999), Eucalyptus (Bandyopadhyay et al 1999), Valeriana edulis (Castillo et al 2000), Lathyrus sativus (Zambre et al 2002), Diffenbachia (Shen et al 2007). In most published reports where organogenesis occurred from green organogenic callus, the callus turned green on induction medium, and this is considered a prerequisite for regeneration (Ezura et al 2000;Zambre et al 2002).…”

Section: Discussionsupporting

confidence: 75%

Multiple shoot induction and callus regeneration in Sarcostemma brevistigma Wight & Arnott, a rare medicinal plant

Thomas

2008

Plant Biotechnol Rep

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An efficient micropropagation protocol based on multiple shoot induction and callus regeneration has been standardized in Sarcostemma brevistigma, a rare medicinal plant. The nodal cuttings were cultured on MS medium supplemented with BA (0.5-8 lM) or Kn (0.5-8 lM) alone or in combination with NAA (0.5-1.5 lM). Maximum multiple shoot induction was observed on MS medium supplemented with 4 lM BA. On this medium, 100% cultures responded with an average number of 11.3 shoots per explant. However, the average shoot length was limited to only 0.9 cm on this medium. The addition of 1 lM NAA along with 4 lM BA gave rise to an average number of 10.9 shoots with an average shoot length of 1.8 cm. Luxuriantly growing callus was obtained on MS medium supplemented with BA (5 lM) and 2,4-D (2 lM). The callus was subcultured on MS medium supplemented with BA (2-15 lM) or Kn (2-15 lM) alone or in combination with NAA (0.5-2 lM) for shoot organogenesis. Optimum callus regeneration was obtained on MS medium supplemented with 10 lM BA and 1 lM NAA. On this medium, 100% cultures responded with an average number of 13.4 shoots per culture. The shoots obtained via multiple shoot induction and organogenesis were rooted on halfstrength MS medium supplemented with NAA (1-7 lM) or IBA (1-7 lM). IBA was better than NAA in terms of both the percentage of cultures that responded and the average number of roots per explant. The rooted shoots were successfully transplanted to soil with 86% success.This standardized protocol will help to conserve this rare medicinal plant.

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Section: Discussionsupporting

confidence: 75%

Multiple shoot induction and callus regeneration in Sarcostemma brevistigma Wight & Arnott, a rare medicinal plant

Thomas

2008

Plant Biotechnol Rep

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“…Due to their medical interest as a sedative phytomedicine, rhizomes of different Valeriana species have been overharvested (Castillo et al 2000). Pharmacological properties of Valeriana species (sedative, fungicidal, and antitumoral) have been attributed to various components, such as valepotriates, valerenic acids, borneol derivatives, and flavonoids (Fuzzati et al 1996;Houghton 1999;Fernández et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Improved nutrient medium for biomass and valepotriate production in extended period stock cultures of Valeriana glechomifolia

Maurmann

Rech

Fett-Neto

2008

In Vitro Cell.Dev.Biol.-Plant

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Valeriana glechomifolia, a southern Brazilian endemic species commonly known as Valerian, accumulates the bioactive terpene derivatives valepotriates in all of its organs. In vitro growth of V. glechomifolia on solid Murashige and Skoog (MS) without phytohormones at full, 75% (MS 75), or on a modified formulation (M Δ) was compared in stock cultures kept for up to 9 mo. without subculture. Changes in biomass accumulation, development of roots and shoots, and the production of the valepotriates acevaltrate, didrovaltrate, and valtrate were monthly evaluated. The highest biomass accumulation and root development was observed in plants grown on M Δ, whereas better leaf development was detected in M-Δ-and MSmedium-grown plants after 8 and 9 mo. of culture, respectively. Maximal didrovaltrate and valtrate yields were observed in M-Δ-grown plants harvested after 5 and 6 mo. of culture, respectively, whereas acevaltrate concentration was highest on M-Δ-and MS-75-grown plants after 7 mo. of culture. Plants grown for 6 mo. without subculture in M Δ were successfully propagated, showing stable growth and valepotriate yields three-to sixfold higher that those observed in field-grown plants. The results showed a positive effect of combined moderate reduction in salt concentration and increases in selected micronutrients and myo-inositol amounts on both growth and valepotriate yields of extended period stock cultures of V. glechomifolia.

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“…To evaluate the potential of hairy root production of Verbena officinalis under in vitro conditions, the regeneration by indirect organogenesis was also conducted (Zebarjadi et al, 2011). Previously, plant regeneration has been reported from callus and suspension cultures of Valeriana edulis (Castillo et al, 2000) and Valeriana wallichii (Mathur, 1991).…”

Section: Introductionmentioning

confidence: 99%

Callus induction and plant regeneration of Valeriana officinalis are affected by different leaf explants and various concentrations of plant growth regulators

Zamini¹,

Mokhtari²,

Tansaz³

et al. 2016

bta

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Valeriana officinalis, commonly known as valerian, is native to Europe and Asia and has economic importance as a medicinal plant. The effects of auxins (2,4-D and NAA) on callus induction were studied in vitro using basal piece of leaf + petiole (L1) and middle piece of leaf (L2) ). The results revealed that the effect of explant type on callogenesis was also significant. In contrast to KIN or TDZ, addition of IBA to BAP showed positive synergistic effect for plant regeneration and shoot number per callus.

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Plant regeneration from callus and suspension cultures of Valeriana edulis ssp. procera via simultaneous organogenesis and somatic embryogenesis

Cited by 36 publications

References 12 publications

Multiple shoot induction and callus regeneration in Sarcostemma brevistigma Wight & Arnott, a rare medicinal plant

Multiple shoot induction and callus regeneration in Sarcostemma brevistigma Wight & Arnott, a rare medicinal plant

Improved nutrient medium for biomass and valepotriate production in extended period stock cultures of Valeriana glechomifolia

Callus induction and plant regeneration of Valeriana officinalis are affected by different leaf explants and various concentrations of plant growth regulators

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