Callus induction and plant regeneration of Valeriana officinalis are affected by different leaf explants and various concentrations of plant growth regulators

Abstract

Valeriana officinalis, commonly known as valerian, is native to Europe and Asia and has economic importance as a medicinal plant. The effects of auxins (2,4-D and NAA) on callus induction were studied in vitro using basal piece of leaf + petiole (L1) and middle piece of leaf (L2) ). The results revealed that the effect of explant type on callogenesis was also significant. In contrast to KIN or TDZ, addition of IBA to BAP showed positive synergistic effect for plant regeneration and shoot number per callus.

Interaction Effect of Auxin and Cytokinin on <i>in Vitro</i> Shoot Regeneration and Rooting of Endangered Medicinal Plant <i>Valeriana jatamansi</i> Jones through Tissue Culture

“…Regarding shoot emergence, the results revealed that lower concentrations of both 6-benzyl amino purine (BAP) and α-naphthalene acetic acid (NAA) significantly have positive effect on shoot emergence (Table 1). These findings are in consistent with the results of Zamini et al [10] who reported that the excellent regeneration (82.5%), shoot length (5.96 cm) and shoot number (5.87) of Valeriana officinalis was resulted at lower level of BAP 0.5 mg/L along with IBA 0.5 mg/L. Excellent shoot regeneration percentage (60.00%) was also reported by Zebarjadi et al [14] with 0.5 mg/L NAA + 2 mg/L BAP in Valeriana officinalis.…”

“…Zamini et al [10] organized a method for callus initiation of Valeriana officinalis by using NAA and 2,4-D. Excellent induction of callus (95.83%) resulted with KIN 1 mg/L along with 2,4-D 1.5 mg/L.…”

In vitro propagation and evaluation of Valtrate and Valereinic acid in Valerina officinalis L.

“…Calli fresh and dry weights (g/jar). Data tabulated in Table (1) clearly show that calli fresh and dry weights of leaf, stem, and root explants of Valerina officinalis L. Data showed significant differences between the varied medium of different growth regulators concentrations, explants compared to the control medium. The tested leaf, stem, and root explants cultured on MS+5 ppm BA; MS+5 ppm D; MS+5ppm BA+3ppm The same letters in each column mean non-significant difference; Each value is the average of 3 replicates.…”

“…The Valeriana genus is a member of the Valerianaceae family, and has 419 species. Valeriana officinalis L. (Valerian), is indigenous to Europe and Asia and widely distributed in Chile, Brazil, South Africa, the United States, and subtropical Asia, and is regarded as the most commercially significant species [1,2].…”

“…Utilizing MS+5.0 mg/L BA+0.2 mg/L Naphthaline acetic acid (NAA) produced calli. However, regeneration was accomplished by using Indole butyric acid (IBA) and BA, which had favourable effects on plant regeneration and shoot induction [1]. However, utilizing BA (1mg/l) or (0.5mg/l) resulted in the longest shoots and roots were detected [13,14].…”