Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Querem robur L.) and linden (Tilia cordata Mill.)

Chalupa, V.

doi:10.1007/bf00232408

Cited by 59 publications

(40 citation statements)

References 4 publications

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“…Ishii et al (1999) transferred 10 plantlets from somatic embryos of Q. serrata, eight of which survived. Chalupa (1990) obtained 78% survival from 70 Q. robur and 62 from Q. petraea plantlets. Kim et al (1997) produced 33 plantlets of Q. acutissima, 20 of which were transplanted into pots, 12 survived in the greenhouse and eight in the field.…”

Section: Acclimation Of Cork Oak Plantletsmentioning

confidence: 97%

“…This phenomenon, called autoembryony, is frequent in species such as cork oak, and is undesirable during maturation and later stages. This problem has been detected in related Quercus species (Chalupa, 1990). The addition of abscisic acid (ABA) to the maturation medium partially solves that problem, like in Q. ilex L. (Mauri and Manzanera, 2004) and in Q. suber L. (García-Martin et al, 2005).…”

Section: Effect Of Amino Acids and Activated Charcoal On Embryo Growthmentioning

confidence: 99%

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Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

Pintos¹,

Manzanera

Bueno³

2010

Ann. For. Sci.

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Abstract• Development of both somatic and gametic embryogenesis has many applications in clonal forestry and genetic improvement, for instance as mass-propagation of genetically improved plants and production of pure lines through doubled-haploid plant regeneration from gametic embryos.• The goal of this work was to improve growth, maturation and plantlet regeneration of cork oak (Quercus suber L.) embryos from both somatic and gametic origin.• Activated charcoal promoted a significant increase in growth in terms of relative size and weight of both somatic and doubled-haploid embryos, as well as a more efficient control of secondary somatic embryogenesis during development. A significant interaction was also observed with amino acid nutrition. While some amino acids (i.e., glutamine, arginine or asparagine) did not show significant differences with the controls, a mixture of these three amino acids or gamma amino butyric acid stimulated embryo growth. The highest survival rate during acclimation of plantlets from both somatic and doubled-haploid origin was obtained when the embryos had been previously cultured on basal medium with 3% sucrose and 1% activated charcoal for two months at 4 • C and germinated on medium supplemented with 6-benzylaminopurine and indole-3-butyric acid.• We obtained more than 900 cork oak plantlets acclimated from several embryogenic lines, with a high survival rate, demonstrating that this methodology is applicable for large scale plantlet production. We also report the first regeneration of doubled-haploid plantlets in cork oak. • Le développement des deux embryogenèses gamétique et somatique a de nombreuses applications dans la foresterie clonale et l'amélioration génétique, par exemple en tant que propagation en masse de plants génétiquement améliorés et la production de lignées pures par régénération de plants haploïdes doublés d'embryons gamétiques. Mots-clés• L'objectif de ce travail était d'améliorer la croissance, la maturation et la régénération des plantules des embryons de chêne-liège (Quercus suber L.) des deux origines gamétique et somatique.• Le charbon actif a favorisé une augmentation significative de la croissance en termes de taille et de poids des embryons haploïdes doublés et somatiques, ainsi qu'un contrôle plus efficace de l'embryogenèse somatique secondaire au cours du développement. Une interaction significative a également été observée avec la nutrition aminoacide. Alors que certains acides aminés (c'est-à-dire, la glutamine, l'arginine ou l'asparagine) ne montrent pas de différences significatives avec les témoins, un mélange de ces trois acides aminés ou l'acide butyrique amino gamma a stimulé la croissance de l'embryon. Le taux de survie le plus élevé au cours de l'acclimatation des plantules d'origine à la fois somatique et haploïde doublé, a été obtenu lorsque les embryons ont été auparavant cultivés sur milieu de base à 3 % de saccharose et de 1 % de charbon actif pendant deux mois à 4 • C et ont germé sur un milieu complété par la 6-benzylaminopurine et ...

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Section: Acclimation Of Cork Oak Plantletsmentioning

confidence: 97%

Section: Effect Of Amino Acids and Activated Charcoal On Embryo Growthmentioning

confidence: 99%

Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

Pintos¹,

Manzanera

Bueno³

2010

Ann. For. Sci.

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“…Thus, in cork oak, secondary embryogenesis appeared to be associated with the embryo axis. The absence of secondary embryogenesis in the cotyledons of somatic embryos has been shown in several species, such as Aesculus hippocastanum (Profumo et al 1986), Quercus robur, and Tilia cordata (Chalupa 1990). In assays with zygotic embryos of Pisum sativum, the formation of embryogenic callus was associated exclusively with the embryonic axis and was independent of the maturation of embryos and the combinations and/or concentrations of PGRs used (Kysely and Jacobsen 1990).…”

Section: Introductionmentioning

confidence: 99%

Histology of Organogenic and Embryogenic Responses in Cotyledons of Somatic Embryos of Quercus suber L.

Puigderrajols

Celestino

Suils

et al. 2000

International Journal of Plant Sciences

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. In cork oak (Quercus suber L.), recurrent embryogenesis is produced in vitro through autoembryony without exogenous plant growth regulators (PGRs); secondary embryos appear on the embryo axis but seldom on cotyledons. Focusing mainly on the histological origin of neoformations, we investigated the influence of the embryo axis and exogenous PGRs on the embryogenic potential of somatic embryo cotyledons. Isolated cotyledons of somatic embryos became necrotic when cultured on PGR-free medium but gave secondary embryos when cultured on media containing benzyladenine and naphthaleneacetic acid. Cotyledons of cork oak somatic embryos are competent to give embryogenic responses. Isolated cotyledons without a petiole showed a lower percentage of embryogenic response than did those with a petiole. In petioles, somatic embryos arose from inner parenchyma tissues following a multicellular budding pattern. Joined to the embryo axis, cotyledons did not show morphogenic responses when cultured on PGR-free medium but revealed budlike and phylloid formations when cultured on medium with PGRs. The different morphogenic behavior displayed by somatic cotyledons indicates an influence of the embryo axis and indicates a relationship between organogenic and embryogenic regeneration pathways. HISTOLOGY OF ORGANOGENIC AND EMBRYOGENIC RESPONSES IN COTYLEDONS OF SOMATIC EMBRYOS OF QUERCUS SUBER L.

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“…To solve these difficulties, somatic embryogenesis has also been assayed in this oak. First results in this species were obtained by Chalupa (1990), who reported successful plantlet regeneration from immature embryos cultured on MS or Woody Plant Medium (WPM; Lloyd and McCown, 1981), supplemented with BA 4.4 µM, alone or combined with 2.9 µM gibberellic acid (GA 3 ) or indole-butyric acid (IBA) 0.5 to 5 µM. Similar results were obtained by Hubner et al (1995) on medium supplemented with 1 µM BA + 1 µM 2,4-D.…”

Section: Somatic Embryogenesis In Quercus Roburmentioning

confidence: 99%

Embryogenesis in Oak species. A review

Gómez-Garay¹,

Manzanera

Pintos-Lopez³

2014

For. syst.

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Aim of study: A review on the propagation methods of four Quercus species, namely Q. suber, Q. robur, Q. ilex and Q. canariensis, through somatic embryogenesis and anther embryogenesis are presented.Area of study: The study comprises both Mediterranean and Atlantic oak species located in Spain.Material and Methods: Somatic embryogenesis was induced on immature zygotic embryos of diverse oak species, permitting the multiplication of half-sib families. Induction of haploid embryos and doubled haploids was assayed in both Q. suber and Q. ilex by temperature stress treatments of anthers containing late vacuolated microspores. The haploid origin of the anther embryos has been evaluated by quantitative nuclear DNA analysis through flow cytometry and by DNA microsatellite markers. Genetic transformation of cork oak has also been performed by means of Agrobacterium tumefaciens vectors. Proteomic analysis has been conducted to screen the diverse protein profiles followed by in vitro derived embryos during their development.Research highlights: Successful plant regeneration from both somatic and haploid embryos has been achieved. In the particular case of cork oak, doubled-haploid plants were obtained. Plantlets regenerated from selected parent trees through somatic embryogenesis were acclimated in the greenhouse and in the nursery, and were planted in an experimental plot in the field. Preliminary evaluation of the cork quality of the plants showed a good heritability correlation with the parent trees. This article revises the work of and is dedicated to Dr. M.A. Bueno, who devoted much of her professional life to the research on Biotechnology and Genetics of forest species, leading the Laboratory of Forest Biotechnology at the Spanish Institute of Agronomic Research (INIA).Key words: anther embryogenesis; microspore; pollen; Quercus canariensis; Quercus ilex; Quercus robur; Quercus suber; somatic embryogenesis.

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Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Querem robur L.) and linden (Tilia cordata Mill.)

Cited by 59 publications

References 4 publications

Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

Histology of Organogenic and Embryogenic Responses in Cotyledons of Somatic Embryos of Quercus suber L.

Embryogenesis in Oak species. A review

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