Plant Propagation through Tissue Culture – A Biotechnological Intervention

Lone, Sameena Maqbool; Hussain, Khalid; Malik, Ajaz Ahmad; Magray, Mudasir; Hussain, Syed Mazahir; Rashid, Majid; Farwah, Syeda

doi:10.20546/ijcmas.2020.907.254

Cited by 6 publications

(4 citation statements)

References 3 publications

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“…Increasing demand for agricultural commodities led scientists to discover techniques to enhance production with excellent nutritional value by utilising contemporary technology; Plant tissue culture is one of these techniques presented by scientists as a solution to the growing demand for agricultural commodities [24,25]. Plant tissue culture requires an appropriate supply of nutrients, a medium pH, a sufficient temperature, and a sufficient gaseous and liquid or hard environment [26,27]. This plant tissue culture strategy protects vulnerable species and is the most successful way for increasing crop yield and quality.…”

Section: Journal Of Asian Scientific Researchmentioning

confidence: 99%

The Influence of Different Concentrations of Plant Hormones in Vitro on Seeds Germination of Fenugreek (Trigonella Foenum-Graecum)

Agha

Sidik

Radzun

et al. 2022

5003

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Trigonella foenum-graecum is an important leguminous plant used in medicine and human health since ancient times, and the various civilizations of the world, as the Egyptian, Greek, and Indian civilizations, have been used in traditional medicine. Trigonella foenum-graecum contains a wide range of pharmacological effects because of the presence of several beneficial plant secondary metabolites. However, the resistant nature of this plant causes major challenges in in vitro multiplication, emphasizing the need to assess novel approaches for the propagation of this plant. As a result, this study reports on the effects of BAP, NAA, and 2,4-D on T. foenum graecum seed germination in vitro. The germination percentage recorded every week for 4 weeks, the parameter's last mean was calculated. The results showed that 2.0 mg/L BAP created the best effect in the parameter studied. The results revealed that 2.0 mg/l BAP provided the greatest response germination percentage (86 ±2.357%). These results highlight the optimum value of growth hormones producing T. foenum graecum plant with a minimum germination rate in a large scale.

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Section: Journal Of Asian Scientific Researchmentioning

confidence: 99%

The Influence of Different Concentrations of Plant Hormones in Vitro on Seeds Germination of Fenugreek (Trigonella Foenum-Graecum)

Agha

Sidik

Radzun

et al. 2022

5003

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“…2,4-D is the main growth regulator in the auxins group. It contributes to the growth and development of the plant cell through its ability to elongate it and its essential synergistic roles in which it participates in plant growth regulators [7]. BA is the most important of the cytokinins.…”

Section: Introductionmentioning

confidence: 99%

2,4-Dichlorophenoxyacetic Acid and 6-Benzyladenine Stimulate of Indication and Biomass Accumulation in a Phaseolus Vulgaris Callus Culture

Rashid,

Neamah

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Obtaining callus tissue is one of the essential aims of plant tissue culture technology due to its great importance in subsequent experiments. The present study aimed to determine the interactive effects of plant growth regulators in stimulating calli cultures from the hypocotyl of Phaseolus vulgaris. The study was conducted in the Plant Tissue Culture Laboratory, Desert Studies Center, University of Anbar. Different concentrations of 2,4-D were used, including 1.0, 2.0, 3.0, and 4.0 mg L−1, with concentrations of BA including 0, 0.5, 1.0, 1.5, and 2.0 mg L−1. Sodium hypochlorite (NaOCl) was used to obtain sterile seedlings. The seeds were sterilized with four concentrations of 0, 2, 4 and 6% for 5, 10 and 15 min. We use the complete random design (RCD) for coefficient distribution. The results showed the mixture of NaOCl 4.0% for 10 min recorded the highest percentage of sterile seedling production (SSP) at 90%. As for the induction of callus culture, the hypocotyl extracted from sterilized seedlings was grown in MS medium supplemented with 2,4-D and BA concentrations for 21 days. The results showed that the combination 2.0+1.5 mg L−1 caused the highest callus formation response (CFR) by 100%, the lowest complete callus formation (CCF) by 5.80 days, and the highest fresh weight (FW) by 4.318 g. At the same time, the combination of 2.0+2.0 mg L−1 reduced the start of callus formation (SCF) by 3.40 days.

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“…PTC relies on a phenomenon known as totipotency, which allows a single plant cell to grow, divide and differentiate into a complete new plant. PTC involves the cultivation of cells, tissues, organs in an appropriate nutrient medium, in a sterile environment to facilitate growth and maintain it, in vitro (Thorpe, 2007;George et al, 2008;Lone et al, 2020). PTC techniques have made it possible to eliminate plant pathogens like bacteria and fungus.…”

Section: Introductionmentioning

confidence: 99%

Efficient conditions for in vitro establishment and regeneration of disease-free Ugandan farmer-preferred cassava genotypes

Apio¹,

Alicai²,

Ogwok³

2021

Afr. J. Biotechnol.

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Cassava (Manihot esculentaCrantz) is majorly devastated by two viral diseases, cassava brown streak disease (CBSD) and cassava mosaic disease (CMD), resulting in 100% yield loss. Being a clonal plant, nodal cuttings (NC) and shoot apical meristems (SAMs) are the best explants for production of disease free planting materials. In this study, NCs and SAMs were used to determine reliable indicators for successful in vitro establishment of cassava. Eight cassava genotypes were used for the study. Leaf samples were collected from 30 stakes of each of the eight genotypes planted in the screen house. The leaf samples were pooled and screened for presence and/or absence of CBSD and CMD by PCR using virus specific primers. Nodal cuttings were excised from screen house grown plants, surface sterilized to rid-off contaminants and established on Murashige and Skoog (MS) Medium. Using the sprouted stakes, 5-mm sized SAMs were excised, surface sterilized and reduced to 0.5-1 and 2-3 mm sizes. The SAMs were established on MS medium with varying concentrations of plant growth regulators (0.5, 1, 2) ml/L Benzylaminopurine (BAP) and ( 2, 4) ml/L Naphthalene acetic acid (NAA), Kinetin (K) and BAP respectively. PCR results revealed the pooled leaf samples were free of both CBSD and CMD for all genotypes. Establishment and regeneration of NCs was possible with MS medium for all genotypes. For the SAMs, the concentrations of (2, 4) ml/LBAP followed by 2 ml/LNAA facilitated their establishment and regeneration in comparison to KIN.SAMs of 2-3 mm sizes regenerated better than 0.5 -1 mm size. Both NCs and SAMs of the different genotypes produced leaves, nodes, roots and there was an increase in plant length. These parameters are critical indicators for in vitro establishment and regeneration of cassava.

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Plant Propagation through Tissue Culture – A Biotechnological Intervention

Cited by 6 publications

References 3 publications

The Influence of Different Concentrations of Plant Hormones in Vitro on Seeds Germination of Fenugreek (Trigonella Foenum-Graecum)

The Influence of Different Concentrations of Plant Hormones in Vitro on Seeds Germination of Fenugreek (Trigonella Foenum-Graecum)

2,4-Dichlorophenoxyacetic Acid and 6-Benzyladenine Stimulate of Indication and Biomass Accumulation in a Phaseolus Vulgaris Callus Culture

Efficient conditions for in vitro establishment and regeneration of disease-free Ugandan farmer-preferred cassava genotypes

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