Plant Polyphenols Stimulate Adhesion to Intestinal Mucosa and Induce Proteome Changes in the Probiotic <i>Lactobacillus acidophilus</i> NCFM

Çelebioğlu, Hasan Ufuk; Delsoglio, Marta; Brix, Susanne; Pessione, Enrica; Svensson, Birte

doi:10.1002/mnfr.201700638

Cited by 36 publications

(38 citation statements)

References 68 publications

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“…Lactobacillus acidophilus LA-5 and Lactobacillus rhamnosus GG, which are kind gifts of Chr. Hansen, Turkey, were grown in Man, Rogosa and Sharpe (MRS) medium without shaking, at 37ºC (Celebioglu, Delsoglio, Brix, Pessione, & Svensson, 2018). The bacteria were divided into groups and treated with ipolamiide.…”

Section: Growth Of Probiotic Bacteria In the Presence Of Ipolamiide Amentioning

confidence: 99%

İpolamiidin Lactobacillus acidophilus and Lactobacillus rhamnosus Bakterilerinin Probiyotik Özellikleri Üzerine Etkileri

Taş

Köroğlu

Çelebioğlu

et al. 2019

European Journal of Science and Technology

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Iridoid glycosides are 2-cyclopentanoid derivatives of terpene origin naturally occuring in the leaves, fruits, seeds, bark, roots of various plants. They are medically important because used in the treatment of many diseases while normally protect plants from biotic and abiotic attacks. They have anti-microbial, anti-tumor, anti-cardiac and anti-inflammatory effects. Ipolamiide is one of the iridoid glycosides and naturally present in many plants. Ipolamiide is very little known compound in terms of the biological activities. The beneficial microorganisms in the body and the plant secondary metabolites can interact in the human gastrointestinal tract. Probiotics are live microorganisms that have many health benefits by improving microbial balance of the intestines. Among the most known and most studied probiotics are Lactobacillus acidophilus and Lactobacillus rhamnosus. The aim of the present study is to investigate the effects of ipolamiide on probiotic bacteria Lactobacillus rhamnosus GG (GG) and Lactobacillus acidophilus LA-5 (LA-5). For this purpose, ipolamiide was added to the growth of probiotics in different concentrations and its effects on bacterial growth kinetics, bacterial surface hydrophobicity (Microbial Adhesion to Solvents-MATS Test) and bacterial aggregation (Auto-Aggregation Test) were investigated. The results showed that ipolamiide did not show any important change in surface hydrophobicity of probiotic bacteria. Dose-dependent increases in auto-aggregation properties of the LA-5 and GG were observed. However, further detailed studies are required to give insight into other possible biological activities of ipolamiide.

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Section: Growth Of Probiotic Bacteria In the Presence Of Ipolamiide Amentioning

confidence: 99%

İpolamiidin Lactobacillus acidophilus and Lactobacillus rhamnosus Bakterilerinin Probiyotik Özellikleri Üzerine Etkileri

Taş

Köroğlu

Çelebioğlu

et al. 2019

European Journal of Science and Technology

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“…It is hypothesized that the utilization of oligosaccharides can modify the abundance of probiotic bacterial proteins important for adhesion to the GIT mucosa. Adhesion to mucin or HT-29 cells was assayed essentially according to the method based on labelling of bacteria with a fluorophore, allowing quantification of adhered cells [ 51 ] as reported for L. acidophilus NCFM [ 44 , 45 , 85 ] (refer to Section 4.1 ).…”

Section: Dietary Nutrients-lactobacillus Interactions Characterizementioning

confidence: 99%

“…Lactobacillus acidophilus NCFM grown on different carbon sources, including approved and emerging prebiotics, and using glucose as reference, demonstrated a connection between the in vitro adhesion capacity to a mucin coating or a confluent HT-29 cell layer, respectively, ( Table 1 ) and relative abundancy changes in bacterial proteomes ( Table 2 ) [ 44 , 45 , 85 ]. The carbon sources included raffinose [ 44 ], fructooligosaccharides (FOS), galactooligosaccharides (GOS), lactulose, cellobiose, melibiose, palatinose, trehalose, and polydextrose [ 45 ].…”

Section: Dietary Nutrients-lactobacillus Interactions Characterizementioning

confidence: 99%

Dietary Nutrients, Proteomes, and Adhesion of Probiotic Lactobacilli to Mucin and Host Epithelial Cells

Çelebioğlu

Svensson

2018

Microorganisms

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The key role of diet and environment in human health receives increasing attention. Thus functional foods, probiotics, prebiotics, and synbiotics with beneficial effects on health and ability to prevent diseases are in focus. The efficacy of probiotic bacteria has been connected with their adherence to the host epithelium and residence in the gut. Several in vitro techniques are available for analyzing bacterial interactions with mucin and intestinal cells, simulating adhesion to the host in vivo. Proteomics has monitored and identified proteins of probiotic bacteria showing differential abundance elicited in vitro by exposure to food components, including potential prebiotics (e.g., certain carbohydrates, and plant polyphenols). While adhesion of probiotic bacteria influenced by various environmental factors relevant to the gastrointestinal tract has been measured previously, this was rarely correlated with changes in the bacterial proteome induced by dietary nutrients. The present mini-review deals with effects of selected emerging prebiotics, food components and ingredients on the adhesion of probiotic lactobacilli to mucin and gut epithelial cells and concomitant abundancy changes of specific bacterial proteins. Applying this in vitro synbiotics-like approach enabled identification of moonlighting and other surface-located proteins of Lactobacillus acidophilus NCFM that are possibly associated with the adhesive mechanism.

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“…Food components provide the necessary nutrient for GI microbiota and they also determine adhesion and colonization of microbes on GI surface [51,52]. Animal fats preferentially support growth and establishment of Bacteroides, whereas carbohydrates encourage the Prevotella [18,53].…”

Section: Microbe-diet Interactionmentioning

confidence: 99%

Personalized Probiotics Based on Phenotypes and Dietary Habit: A Critical Evaluation

Lee¹

2018

J Prob Health

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It is now established that probiotics are strain-dependent, to further advance the concept of probiotic in the maintenance of health and treatment of dysbiosis, the scope of personalized probiotics need to be defined. Currently, probiotic products are marketed worldwide, with the assumption that probiotics with demonstrated health effects work on all people, irrespective of the genetic (ethnicity), environment (geographical location), dietary habit and life style. The effectiveness of probiotics are determined by 1) interactions with prevailing gastrointestinal (GI) commensal microbes, in promoting the establishment of beneficial microbes and elimination of pathogens, 2) Interaction with the host, in achieving a desirable probiotic effect, 3) Interaction with diet, to survive, proliferate and colonize GI, albeit temporary, and in the production of beneficial bioactive metabolites, such as short-chain-fatty acids (e.g. butyric acid), bile acid derivatives and trimethylamines. The benefits acquired from a probiotic are personal, depending on the health status, dietary habit and prevailing GI microbiota. Personalized probiotics should be established to achieve precision administration of specific probiotic effects for targeted population. Globalization and urbanization of human activities have led to merging of dietary habit, thus effective probiotics should evolve in tandem. Ultimately the probiotics of choice should be directed at specific physiological stage, health condition and targeted diseases.

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Plant Polyphenols Stimulate Adhesion to Intestinal Mucosa and Induce Proteome Changes in the Probiotic Lactobacillus acidophilus NCFM

Abstract: The five plant phenolics found in various foods stimulate the adhesive capacity of NCFM in diverse ways and elicit relative abundancy changes of specific proteins, providing molecular level insight into the mechanism of the putative beneficial effects of the polyphenols.

Cited by 36 publications

References 68 publications

İpolamiidin Lactobacillus acidophilus and Lactobacillus rhamnosus Bakterilerinin Probiyotik Özellikleri Üzerine Etkileri

İpolamiidin Lactobacillus acidophilus and Lactobacillus rhamnosus Bakterilerinin Probiyotik Özellikleri Üzerine Etkileri

Dietary Nutrients, Proteomes, and Adhesion of Probiotic Lactobacilli to Mucin and Host Epithelial Cells

Personalized Probiotics Based on Phenotypes and Dietary Habit: A Critical Evaluation

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