Plant Pathogens Utilize Effectors to Hijack the Host Endoplasmic Reticulum as Part of Their Infection Strategy

Jing, Maofeng; Wang, Yuanchao

doi:10.1016/j.eng.2020.03.003

Cited by 14 publications

(18 citation statements)

References 49 publications

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“…BiP is also the target of some Phytophthora ER-localised effectors, such as PsAvh262 from P. sojae [32]. The interaction BiPeffector has been suggested to decouple the SDF2/ ERdj3B/BiP complex [32,33], which might cause the release and secretion of SDF2 and ERdj3b, with negative consequences for the stable accumulation of PRRs at the plasma membrane and downstream defence responses. PxSDF2L1 chaperone has its ER retrieval signal and could rescue the system while BiP stays occupied with effectors.…”

Section: Discussionmentioning

confidence: 99%

Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae

et al. 2021

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Background The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. Here, we investigate whether a continuous expression of PxSDF2L1 in Nicotiana benthamiana can similarly confer resistance to plant pathogen, particularly, the black shank Phytophthora parasitica var. nicotianae. Results The N. benthamiana plants were inoculated with agrobacteria transformed with a PVX-based binary vector carrying the PxSDF2L1 gene; similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. In pot trials, agroinfected N. benthamiana plants constitutively expressing PxSDF2L1 showed a significant reduction of stem disease symptoms caused by the inoculation with P. parasitica, compared with controls. Conclusions We confirm a role of PxSDF2L1 in resistance to black shank, with a potential application to engineering active resistance against this oomycete in the commercial N. tabacum species and propose its evaluation in other crop families and plant pathogens.

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Section: Discussionmentioning

confidence: 99%

Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae

et al. 2021

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“…If the protein accumulation exceeds the ER capacity, the cell responds by upregulating specific ER-associated genes and enzymes to restore homeostasis. This process is termed the ER stress response ( Howell, 2013 ; Jing and Wang, 2020 ). Plant ER stress sensors can recognize ER stress and trigger the ER stress response, also called the unfolded protein response (UPR).…”

Section: Discussionmentioning

confidence: 99%

Combined analysis of the transcriptome and proteome of Eucommia ulmoides Oliv. (Duzhong) in response to Fusarium oxysporum

Dong

Huang

et al. 2022

Front. Chem.

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Eucommia ulmoides Oliv. (Duzhong), a valued traditional herbal medicine in China, is rich in antibacterial proteins and is effective against a variety of plant pathogens. Fusarium oxysporum is a pathogenic fungus that infects plant roots, resulting in the death of the plant. In this study, transcriptomic and proteomic analyses were used to explore the molecular mechanism of E. ulmoides counteracts F. oxysporum infection. Transcriptomic analysis at 24, 48, 72, and 96 h after inoculation identified 17, 591, 1,205, and 625 differentially expressed genes (DEGs), while proteomics identified were 66, 138, 148, 234 differentially expressed proteins (DEPs). Meanwhile, GO and KEGG enrichment analyses of the DEGs and DEPs showed that they were mainly associated with endoplasmic reticulum (ER), fructose and mannose metabolism, protein processing in the ER, type II diabetes mellitus, the ribosome, antigen processing and presentation, and the phagosome. In addition, proteome and transcriptome association analysis and RT-qPCR showed that the response of E. ulmoides to F. oxysporum was likely related to the unfolded protein response (UPR) of the ER pathway. In conclusion, our study provided a theoretical basis for the control of F. oxysporum.

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“…Calreticulin (CRT) is a highly conserved calcium-binding molecular chaperone that facilitates the folding of newly synthesized glycoproteins and regulates Ca 2+ homeostasis in the endoplasmic reticulum (ER) lumen 98 , 99 . It has been reported that CRT isoforms (CRT1, CRT2, and CRT3) are important regulators of plant innate immunity.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola

et al. 2021

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Wild grapevines can show strong resistance to the downy mildew pathogen P. viticola, but the associated mechanisms are poorly described, especially at early stages of infection. Here, we performed comparative proteomic analyses of grapevine leaves from the resistant genotype V. davidii “LiuBa-8” (LB) and susceptible V. vinifera “Pinot Noir” (PN) 12 h after inoculation with P. viticola. By employing the iTRAQ technique, a total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN, respectively. The majority of these DEPs were related to photosynthesis, respiration, cell wall modification, protein metabolism, stress, and redox homeostasis. Compared with PN, LB showed fewer downregulated proteins associated with photosynthesis and more upregulated proteins associated with metabolism. At least a subset of PR proteins (PR10.2 and PR10.3) was upregulated upon inoculation in both genotypes, whereas HSP (HSP70.2 and HSP90.6) and cell wall-related XTH and BXL1 proteins were specifically upregulated in LB and PN, respectively. In the incompatible interaction, ROS signaling was evident by the accumulation of H2O2, and multiple APX and GST proteins were upregulated. These DEPs may play crucial roles in the grapevine response to downy mildew. Our results provide new insights into molecular events associated with downy mildew resistance in grapevine, which may be exploited to develop novel protection strategies against this disease.

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Plant Pathogens Utilize Effectors to Hijack the Host Endoplasmic Reticulum as Part of Their Infection Strategy

Cited by 14 publications

References 49 publications

Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae

Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae

Combined analysis of the transcriptome and proteome of Eucommia ulmoides Oliv. (Duzhong) in response to Fusarium oxysporum

Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola

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