Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola

Abstract

Wild grapevines can show strong resistance to the downy mildew pathogen P. viticola, but the associated mechanisms are poorly described, especially at early stages of infection. Here, we performed comparative proteomic analyses of grapevine leaves from the resistant genotype V. davidii “LiuBa-8” (LB) and susceptible V. vinifera “Pinot Noir” (PN) 12 h after inoculation with P. viticola. By employing the iTRAQ technique, a total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN, respectively. The majority of these DEPs were related to photosynthesis, respiration, cell wall modification, protein metabolism, stress, and redox homeostasis. Compared with PN, LB showed fewer downregulated proteins associated with photosynthesis and more upregulated proteins associated with metabolism. At least a subset of PR proteins (PR10.2 and PR10.3) was upregulated upon inoculation in both genotypes, whereas HSP (HSP70.2 and HSP90.6) and cell wall-related XTH and BXL1 proteins were specifically upregulated in LB and PN, respectively. In the incompatible interaction, ROS signaling was evident by the accumulation of H2O2, and multiple APX and GST proteins were upregulated. These DEPs may play crucial roles in the grapevine response to downy mildew. Our results provide new insights into molecular events associated with downy mildew resistance in grapevine, which may be exploited to develop novel protection strategies against this disease.

Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean

“…The main documented responses so far are the local deposition of CW materials, known as papillae or the cross-linking among components of the matrix, in order to hinder the access of the pathogens to the protoplast [ 22 , 31 , 32 ]. Recently, several transcriptomic and proteomic analysis would indicate that CW remodeling is a widely extended process in nature [ 33 , 34 , 35 , 36 ], however the detailed characterization of the changes that take place in muro after bacteria attack remain poorly characterized.…”

CsWRKY25 Improves Resistance of Citrus Fruit to Penicillium digitatum via Modulating Reactive Oxygen Species Production

“…By using DLR and EMSA assays, infection site (Torres and Dangl, 2005;Lehmann et al, 2015;Camejo et al, 2016;Segal and Wilson, 2018). And as a signal molecule, it participates in the strengthening of cell wall and promotes pathogenesis-related genes (PRs) (Liu et al, 2021).…”

“…NADPH oxidase enzymes encoded by the Rboh gene could cause the accumulation of ROS, which is critical in the defense system of plants, because it can inhibit the pathogens infection process by directly inhibiting pathogen or inducing hypersensitivity at the infection site ( Torres and Dangl, 2005 ; Lehmann et al, 2015 ; Camejo et al, 2016 ; Segal and Wilson, 2018 ). And as a signal molecule, it participates in the strengthening of cell wall and promotes pathogenesis-related genes ( PRs ) ( Liu et al, 2021 ).…”

Transcriptional Profiling of Resistant and Susceptible Cultivars of Grapevine (Vitis L.) Reveals Hypersensitive Responses to Plasmopara viticola

“…Interestingly, Torres et al (2002) research confirmed the absence of the NADPH oxidase genes, AtRbohD and AtRbohF, suppress ROS production and the defense response of Arabidopsis against pathogen attack. ROS levels depend on the balance between ROS production and scavenging, and excess ROS could injure cells ( Mittler et al, 2004 ; Liu et al, 2021 ). ROS amounts depend both on enzymatic and non-enzymatic scavenging molecules such as PrxII, APX, dehydroascorbate reductase (DHAR), and 1-Cys peroxiredoxin (1-CysPrx), which offer a highly efficient system for maintaining ROS homeostasis ( Supplementary Table 6 ).…”