Plant-pathogen interactions: MicroRNA-mediated trans-kingdom gene regulation in fungi and their host plants

Mathur, Monika; Nair, Aswathy; Kadoo, Narendra Y.

doi:10.1016/j.ygeno.2020.05.021

Cited by 25 publications

(15 citation statements)

References 87 publications

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“…MiRNAs regulated the function of target genes either by cleaving or by blocking the protein translation via binding to the 3’ untranslated region (UTR) of the target mRNAs. In addition, miRNAs that assist or curtail the survival of plant pathogens in the host as well as the mechanisms by which plant pathogens modulate the levels of miRNAs in order to multiply in the host are to be deciphered yet ( Weiberg et al, 2013 ; Lin et al, 2016 ; Chopperla et al, 2020 ; Mathur et al, 2020 ; Molla et al, 2020 ; Wang and Dean, 2020 ; Wenlei et al, 2020 ). Therefore, in order to identify the rice miRNA profiles upon R. solani infection, large-scale time series (0, 12, 24, 36, 48, 64, and 72 hpi) sequencing was conducted.…”

Section: Resultsmentioning

confidence: 99%

An Insight Into Pentatricopeptide-Mediated Chloroplast Necrosis via microRNA395a During Rhizoctonia solani Infection

Srikakulam

Guria²,

Karanthamalai³

et al. 2022

Front. Genet.

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Sheath blight (ShB) disease, caused by Rhizoctonia solani, is one of the major biotic stress-oriented diseases that adversely affect the rice productivity worldwide. However, the regulatory mechanisms are not understood yet comprehensively. In the current study, we had investigated the potential roles of miRNAs in economically important indica rice variety Pusa Basmati-1 upon R. solani infection by carrying out in-depth, high-throughput small RNA sequencing with a total data size of 435 million paired-end raw reads from rice leaf RNA samples collected at different time points. Detailed data analysis revealed a total of 468 known mature miRNAs and 747 putative novel miRNAs across all the libraries. Target prediction and Gene Ontology functional analysis of these miRNAs were found to be unraveling various cellular, molecular, and biological functions by targeting various plant defense-related genes. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to validate the miRNAs and their putative target genes. Out of the selected miRNA-specific putative target genes, miR395a binding and its cleavage site on pentatricopeptide were determined by 5’ RACE-PCR. It might be possible that R. solani instigated chloroplast degradation by modulating the pentatricopeptide which led to increased susceptibility to fungal infection.

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Section: Resultsmentioning

confidence: 99%

An Insight Into Pentatricopeptide-Mediated Chloroplast Necrosis via microRNA395a During Rhizoctonia solani Infection

Srikakulam

Guria²,

Karanthamalai³

et al. 2022

Front. Genet.

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“…A similar observation was made on R. solani targeting maize genes [ 25 ]. Recently, cross kingdom regulation of genes by miRNAs during host–pathogen interaction has been demonstrated in several pathosystems [ 41 ]. Bidirectional cross-kingdom transport of microRNAs or small RNAs through naked form, combined with RNA-binding proteins or enclosed by vesicles between fungal pathogens and its host plants has been reported [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Expression Profiling of Small RNAs in Indian Strain of Rhizoctonia solani AG1-1A Reveals Differential Regulation of milRNAs during Pathogenesis and Crosstalk of Gene Regulation

Prathi

Rani

Balachandran

et al. 2021

JoF

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Rhizoctonia solaniAG1-1A is a necrotrophic fungus that causes sheath blight disease in rice. The reliable resistant source against this phytopathogenic fungus is not available in the gene pool of rice. Better understanding of pathogen genomics and gene regulatory networks are critical to devise alternate strategies for developing resistance against this noxious pathogen. In this study, miRNA-like RNAs (milRNAs) of an Indian strain of R. solani were identified by deep sequencing of small RNAs. We identified 128 known and 22 novel milRNAs from 20,963,123 sequence reads. These milRNAs showed 1725 target genes in the fungal genome which include genes associated with growth, development, pathogenesis and virulence of R. solani. Notably, these fungal milRNAs showed their target genes in host (rice) genome also which were later verified by qRT-PCR. The host target genes are associated with auxin metabolism, hypersensitive response, defense genes, and genes related to growth and development of rice. Osa-vacuolar-sorting receptor precursor: Rhi-milR-13, Osa-KANADI1:Rhi-milR-124, Osa-isoflavone reductase: Rhi-milR-135, Osa-nuclear transcription factor Y:Rhi-milR-131, Osa-NB-ARC domain containing protein: Rhi-milR-18, and Osa-OsFBX438: Rhi-milR-142 are notable potential regulons of host target genes: fungal milRNAs that need to be investigated for better understanding of the crosstalk of RNAi pathways between R. solani and rice. The detailed expression analysis of 17 milRNAs by qRT-PCR was analysed during infection at different time points of inoculation, at different growth stages of the host, in four different genotypes of the host, and also in four different strains of fungi which revealed differential regulation of milRNAs associated with pathogenesis and virulence. This study highlights several important findings on fungal milRNAs which need to be further studied and characterized to decipher the gene expression and regulation of this economically important phytopathogen.

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“…sRNA-like molecules from the oomycete pathogen Hyaloperonospora arabidopsidis also use the host RNA interference machinery to reduce transcript levels of defense-related genes in infected plant cells, thereby facilitating infection [100]. miRNA-like RNAs have been identified in several filamentous fungi, including Metarhizium anisopliae [101], S. sclerotiorum [102], Trichoderma reesei [103], and F. oxysporum [104], and some of these RNAs can mimic plant miRNAs and target plant genes [105]. Puccinia striiformis f. sp.…”

Section: Pathogens Secrete Hormone-like Molecules That Interfere With Plant Development and Defense Responsesmentioning

confidence: 99%

Emerging roles of pathogen-secreted host mimics in plant disease development

Wang

Zhang

et al. 2021

Trends in Parasitology

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Plant-pathogen interactions: MicroRNA-mediated trans-kingdom gene regulation in fungi and their host plants

Cited by 25 publications

References 87 publications

An Insight Into Pentatricopeptide-Mediated Chloroplast Necrosis via microRNA395a During Rhizoctonia solani Infection

An Insight Into Pentatricopeptide-Mediated Chloroplast Necrosis via microRNA395a During Rhizoctonia solani Infection

Genome-Wide Expression Profiling of Small RNAs in Indian Strain of Rhizoctonia solani AG1-1A Reveals Differential Regulation of milRNAs during Pathogenesis and Crosstalk of Gene Regulation

Emerging roles of pathogen-secreted host mimics in plant disease development

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