Plant NB-LRR proteins: tightly regulated sensors in a complex manner

Lee, Hyunah; Yeom, Seon-In

doi:10.1093/bfgp/elv012

Cited by 81 publications

(62 citation statements)

References 147 publications

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“…The NBS-encoding R gene family is one of the largest in the H. syriacus genome, with 472 genes, approximately three times greater than A. trichopoda and A. thaliana . These genes are divided into two clades based on presence of the distinct toll interleukin receptor (TIR) domain 50 . TIR genes in H. syriacus ( n = 76, 17%) are markedly over-represented compared to those of S .…”

Section: Resultsmentioning

confidence: 99%

Genome analysis ofHibiscus syriacusprovides insights of polyploidization and indeterminate flowering in woody plants

Kim

Koo

et al. 2016

DNA Res

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Hibiscus syriacus (L.) (rose of Sharon) is one of the most widespread garden shrubs in the world. We report a draft of the H. syriacus genome comprised of a 1.75 Gb assembly that covers 92% of the genome with only 1.7% (33 Mb) gap sequences. Predicted gene modeling detected 87,603 genes, mostly supported by deep RNA sequencing data. To define gene family distribution among relatives of H. syriacus, orthologous gene sets containing 164,660 genes in 21,472 clusters were identified by OrthoMCL analysis of five plant species, including H. syriacus, Arabidopsis thaliana, Gossypium raimondii, Theobroma cacao and Amborella trichopoda. We inferred their evolutionary relationships based on divergence times among Malvaceae plant genes and found that gene families involved in flowering regulation and disease resistance were more highly divergent and expanded in H. syriacus than in its close relatives, G. raimondii (DD) and T. cacao. Clustered gene families and gene collinearity analysis revealed that two recent rounds of whole-genome duplication were followed by diploidization of the H. syriacus genome after speciation. Copy number variation and phylogenetic divergence indicates that WGDs and subsequent diploidization led to unequal duplication and deletion of flowering-related genes in H. syriacus and may affect its unique floral morphology.

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Section: Resultsmentioning

confidence: 99%

Genome analysis ofHibiscus syriacusprovides insights of polyploidization and indeterminate flowering in woody plants

Kim

Koo

et al. 2016

DNA Res

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“…Most plant R genes belong to the NB-LRR superfamily containing a Nucleotide-Binding (NB) domain followed by multiple Leucine-Rich Repeats (LRR) at the C-terminal end. NB-LRR proteins are subdivided into two major groups – TNL (TIR-NB-LRR) and CNL (CC-NB-LRR) on the basis of the presence of either a TIR (similar to animal Toll-like/interleukin-1 receptors) or a CC (coiled-coil) domain at the N-terminus (Michelmore et al , 2013; Lee and Yeom, 2015).…”

Section: Discussionmentioning

confidence: 99%

BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo Candida

Arora

Padmaja

Paritosh

et al. 2019

Preprint

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a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust 36 caused by Albugo candida 37 Running title 38 A CNL type R gene confers white rust resistance in B. juncea 39 Highlight 40 BjuWRR1, a CNL type R gene, was identified from an east European gene pool line of 41 Brassica juncea and validated for conferring resistance to white rust by genetic 42 transformation. 43 Abstract 44 White rust caused by oomycete pathogen Albugo candida is a significant disease of crucifer 45 crops including Brassica juncea (mustard), a major oilseed crop of the Indian subcontinent. 46Earlier a resistance-conferring locus named AcB1-A5.1 was mapped in an east European 47 gene pool line of B. juncea  Donskaja-IV. This line was tested along with some other lines 48 of B. juncea (AABB), B. rapa (AA) and B. nigra (BB) for resistance to six isolates of A. 49 candida collected from different mustard growing regions of India. Donskaja-IV was found 50 to be completely resistant to all the tested isolates. Sequencing of a BAC spanning the locus 51 AcB1-A5.1 showed the presence of a single CC-NB-LRR protein encoding R gene. The 52 genomic sequence of the putative R gene with its native promoter and terminator was used 53 for the genetic transformation of a susceptible Indian gene pool line Varuna and was found to 54 confer complete resistance to all the isolates. This is the first white rust resistance-conferring 55 gene described from Brassica species and has been named BjuWRR1. Allelic variants of the 56 gene in B. juncea germplasm and orthologues in the Brassicaceae genomes were studied to 57 understand the evolutionary dynamics of the BjuWRR1 gene. 58 Introduction 61White rust, caused by an oomycete pathogen Albugo candida (Pers.) Kuntze is a serious 62 disease of economically important Brassica species. A. candida is an obligate biotrophic 63 parasite and rated amongst the top ten oomycete pathogens based on its scientific and 64 economic importance (Kamoun et al., 2015). White rust has been reported to cause 65 A. candida isolates, infection and disease evaluation 129 5White rust infected B. juncea leaves were collected from five different mustard growing 130 regions of north India (Pantnagar, Meerut, Samastipur, Hisar and Alwar) and were purified 131 by performing five passages of inoculations for each isolate on a susceptible B. juncea line, 132Varuna. In each passage, zoosporangia collected from a single pustule were suspended in 200 133 L of sterilized double distilled water followed by drop inoculation on cotyledons of seven-134 day-old seedlings of Varuna. Inoculations were performed in a contained white rust infection 135 chamber maintained at a relative humidity of around 90% and 18°C of temperature with a 16-136 h light/8-h dark cycle. After repeated passages of single pustules, the isolates were 137 maintained on Varuna and the purified A. candida isolates were named as AcP1 (Pantnagar), 138

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“…Plants have multiple layers of defense against pathogen attacks, including preformed barriers for continuous defense and programmed immune responses based on pathogen recognition [20][21][22]. Resistance gene analogs (RGAs) are proteins involved in plant immune responses.…”

Section: Introductionmentioning

confidence: 99%

Development of Clustered Resistance Gene Analogs-Based Markers of Resistance toPhytophthora capsiciin Chili Pepper

Kim

Kang

Lee

et al. 2019

BioMed Research International

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The soil-borne pathogenPhytophthora capsicicauses severe destruction ofCapsicumspp. Resistance inCapsicumagainstP. capsiciis controlled by numerous minor quantitative trait loci (QTLs) and a consistent major QTL on chromosome 5. Molecular markers onCapsicumchromosome 5 have been developed to identify the predominant genetic contributor to resistance but have achieved little success. In this study, previously reported molecular markers were used to reanalyze the major QTL region on chromosome 5 (6.2 Mbp to 139.2 Mbp). Candidate resistance gene analogs (RGAs) were identified in the extended major QTL region including 14 nucleotide binding site leucine-rich repeats, 3 receptor-like kinases, and 1 receptor-like protein. Sequence comparison of the candidate RGAs was performed between twoCapsicumgermplasms that are resistant and susceptible, respectively, toP. capsici.11 novel RGA-based markers were developed through high-resolution melting analysis which were closely linked to the major QTL forP. capsiciresistance. Among the markers, CaNB-5480 showed the highest cosegregation rate at 86.9% and can be applied to genotyping of the germplasms that were not amenable by previous markers. With combination of three markers such as CaNB-5480, CaRP-5130 and CaNB-5330 increased genotyping accuracy for 61Capsicumaccessions. These could be useful to facilitate high-throughput germplasm screening and further characterize resistance genes againstP. capsiciin pepper.

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Plant NB-LRR proteins: tightly regulated sensors in a complex manner

Cited by 81 publications

References 147 publications

Genome analysis ofHibiscus syriacusprovides insights of polyploidization and indeterminate flowering in woody plants

Genome analysis ofHibiscus syriacusprovides insights of polyploidization and indeterminate flowering in woody plants

BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo Candida

Development of Clustered Resistance Gene Analogs-Based Markers of Resistance toPhytophthora capsiciin Chili Pepper

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