Development of Clustered Resistance Gene Analogs-Based Markers of Resistance to<i>Phytophthora capsici</i>in Chili Pepper

Kim, Nayoung; Kang, Won-Hee; Lee, Jundae; Yeom, Seon-In

doi:10.1155/2019/1093186

Cited by 19 publications

(9 citation statements)

References 55 publications

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“…Thus, we examined a total of 102 RNA-seq datasets to analyze the expression of CaRLPs (Supplementary Table S5). Pepper accession ‘CM334,’ which was used for RNA-seq analysis in this study, is known to be resistant to TMV-P0, PepMoV, and P. capsici but susceptible to TMV-P2 45,46 .…”

Section: Resultsmentioning

confidence: 99%

Universal gene co-expression network reveals receptor-like protein genes conferring broad-spectrum resistance in pepper (Capsicum annuum L.)

Kang

et al. 2021

Preprint

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Receptor–like proteins (RLPs) on the plant cell surface have been implicated in immune responses and developmental processes. Although hundreds of RLP genes have been identified in plants, only a few RLPs have been functionally characterized in a limited number of plant species. Here, we identified RLPs in the pepper (Capsicum annuum) genome, and performed comparative transcriptomics coupled with the analysis of conserved gene co–expression networks (GCNs) to reveal the role of core RLP regulators in pepper–pathogen interactions. A total of 102 RNA–seq datasets of pepper plants infected with four pathogens were used to construct CaRLP-targeted GCNs (CaRLP–GCN). All resistance–responsive CaRLP–GCNs were merged to construct a universal GCN. Fourteen hub CaRLPs, tightly connected with defense related gene clusters, were identified in eight modules. Based on the CaRLP–GCNs, we experimentally tested whether hub CaRLPs in the universal GCN are involved in biotic stress response. Of the nine hub CaRLPs tested by virus–induced gene silencing (VIGS), three genes (CaRLP264, CaRLP277, and CaRLP351) showed defense suppression with less hypersensitive response (HR)–like cell death in race–specific and non-host resistance response to viruses and bacteria, respectively, and consistently enhanced susceptibility to Ralstonia solanacearum and/or Phytophthora capsici. These data suggest that key CaRLPs exhibit conserved functions in response to multiple biotic stresses and can be used for engineering of a plant with broad–spectrum resistance. Altogether, we show that generation of a universal GCN using comprehensive transcriptome datasets could provide important clues for uncovering genes involved in various biological processes.

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Section: Resultsmentioning

confidence: 99%

Universal gene co-expression network reveals receptor-like protein genes conferring broad-spectrum resistance in pepper (Capsicum annuum L.)

Kang

et al. 2021

Preprint

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“…In the next step, high-resolution melt (HRM) analysis was performed to validate the SNP markers. The HRM analysis was performed as described by [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Genotyping-by-sequencing-based QTL mapping reveals novel loci for Pepper yellow leaf curl virus (PepYLCV) resistance in Capsicum annuum

Siddique

Lee

Ahn³

et al. 2022

PLoS ONE

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Disease caused by Pepper yellow leaf curl virus (PepYLCV) is one of the greatest threats to pepper (Capsicum spp.) cultivation in the tropics and subtropics. Resistance to PepYLCV was previously identified in a few Capsicum accessions, but no resistance QTLs have been mapped. This study aimed to elucidate the genetics of PepYLCV resistance in C. annuum L. Augmented inoculation by the viruliferous whitefly Bemisia tabaci was used to evaluate parental lines and an F2 segregating population derived from a cross between resistant C. annuum line LP97 and susceptible C. annuum line ECW30R. Final evaluation was performed six weeks after inoculation using a standardized 5-point scale (0 = no symptoms to 4 = very severe symptoms). A high-density linkage map was constructed using genotyping-by-sequencing (GBS) to identify single-nucleotide polymorphism (SNP) markers associated with PepYLCV resistance in the F2 population. QTL analysis revealed three QTLs, peplcv-1, peplcv-7, and peplcv-12, on chromosomes P1, P7, and P12, respectively. Candidate genes associated with PepYLCV resistance in the QTL regions were inferred. In addition, single markers Chr7-LCV-7 and Chr12-LCV-12 derived from the QTLs were developed and validated in another F2 population and in commercial varieties. This work thus provides not only information for mapping PepYLCV resistance loci in pepper but also forms the basis for future molecular analysis of genes involved in PepYLCV resistance.

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“…At present, most of the inbred lines resistant to MRDD in China have been derived from the American hybrid P78599; therefore, the resources for resistance to MRDD in China are relatively narrow (Liu et al 2015). Discovering QTL for resistance to MRDD in elite maize germplasm can be useful for pyramiding disease resistance genes through MAS into different genetic backgrounds and thereby improving breeding efficiency (He et al 2014, Kim et al 2019. Disease resistance in maize is quantitatively inherited, often via QTL.…”

Section: The Genetic Effects Of the Disease Resistance Qtl Qmrdd8 In mentioning

confidence: 99%

Marker-assisted selection of <i>qMrdd8</i> to improve maize resistance to rough dwarf disease

Hua

Wang

et al. 2020

Breed. Sci.

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Maize rough dwarf disease (MRDD) is caused by viruses in the Fijivirus genus in the family Reoviridae. MRDD resistance can be improved by a combination of marker-assisted selection (MAS) and conventional breeding strategies. In our previous study, we fine-mapped a major QTL qMrdd8 and developed the functional Indel marker IDP25K. In the present study, qMrdd8 from the donor parent X178 was introgressed into elite inbred lines derived from the three corn heterotic groups using multi-generation backcrossing and MAS. Recipient lines included Huangzao4, Chang7-2, Ye478, Zheng58, Zhonghuang68, B73, and Ji846. Markers used for foreground selection included IDRQ4, IDRQ47, IDP25K, and IDP27K. Background selection was carried out in the BC 3 or BC 4 using 107 SSR markers to select lines with the highest rate of recovery of the particular recurrent parent genome. Plants from BC 4 F 2 and BC 3 F 2 that carried the shortest qMrdd8 interval from X178 and those with the highest rate of recovery of the recurrent parent genome were then selected to create converted homozygous inbred lines. In 2017, seven converted inbred lines and five hybrids exhibited enhanced resistance to MRDD, while other agronomic traits were not affected under nonpathogenic stress conditions. Thus, the MRDD resistance allele at the qMrdd8 locus, or IDP25K, should be valuable for maize breeding programs in China.

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Development of Clustered Resistance Gene Analogs-Based Markers of Resistance toPhytophthora capsiciin Chili Pepper

Cited by 19 publications

References 55 publications

Universal gene co-expression network reveals receptor-like protein genes conferring broad-spectrum resistance in pepper (Capsicum annuum L.)

Universal gene co-expression network reveals receptor-like protein genes conferring broad-spectrum resistance in pepper (Capsicum annuum L.)

Genotyping-by-sequencing-based QTL mapping reveals novel loci for Pepper yellow leaf curl virus (PepYLCV) resistance in Capsicum annuum

Marker-assisted selection of <i>qMrdd8</i> to improve maize resistance to rough dwarf disease

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