Plant-mediated synthesis of silver nanoparticles using parsley (Petroselinum crispum) leaf extract: spectral analysis of the particles and antibacterial study

Abstract

Synthesis of nanomaterials may involve various routes including physical, chemical and biological approaches. Here, the biological green route was chosen to prepare silver nanoparticles from silver salts to avoid the requirement of costly instruments and involvement of hazardous chemicals as well. To make the process clean and green, leaf extract of parsley (Petroselinum crispum) was used to synthesize Ag nanoparticles at room temperature. The formation of Ag-nanoparticles was monitored by UV-Vis spectroscopy. The presence of silver in the sample and its crystalline nature were verified by X-ray diffraction (XRD) analysis. The size distribution profile and particle size in the suspension were manipulated from dynamic light scattering (DLS) pattern. The shape, size and morphology of the biogenic nanoparticles were studied using high resolution transmission electron microscope (TEM). Fourier transform infra-red spectroscopy was used to detect the biomolecules responsible for reduction of silver ions. These biogenic Ag-nanoparticles showed appreciable antibacterial efficacy against three bacteria-Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus.

Biogenic synthesis of silver nanoparticles using guava (Psidium guajava) leaf extract and its antibacterial activity against Pseudomonas aeruginosa

“…It may be noted that average particle size estimated was 40 nm, and particle size ranges from 10 to 90 nm. The size of these biosynthesized Ag nanoparticles are in conformity with the size of earlier reported biosynthesized Ag nanoparticles using the leaf of Coleus aromaticus, 40-50 nm (Vanaja and Annadurai 2013), using living peanut seedling, 30-100 nm (Raju et al 2014), using leaf extract Petroselinum crispum, 30-32 nm (Roy et al 2014), using Justicia adhatoda L Leaf, 5-50 nm (Bose and Chatterjee 2015), etc.…”

“…Recently, the plant-mediated synthesis of silver nanoparticles using Coleus aromaticus leaf (Vanaja and Annadurai 2013), living peanut seedling (Raju et al 2014), Petroselinum crispum leaf (Roy et al 2014), Citrus limon leaf (Vankar and Shukla 2012), Avicennia marina mangrove plant leaf, bark and root (Gnanadesigan et al 2012) and Justicia adhatoda L. Leaf (Bose and Chatterjee 2015) have been reported.…”

Production of putrescine-capped stable silver nanoparticle: its characterization and antibacterial activity against multidrug-resistant bacterial strains

“…Consequently, diverse ranges of stabilizing or capping materials are used during the synthesis of silver nanoparticles for their controlled release (Liu et al 2010). Arrays of physical, chemical and biological methods have been used to synthesize and stabilize silver nanoparticles (Tolaymat et al 2010;Saha et al 2010Saha et al , 2011Roy et al 2015). Out of these methods, biosynthesis and biomimetic synthesis processes are most extensively accepted in recent years (Vijayaraghavan and Nalini 2010;Mohanpuria et al 2008).…”

Rapid colorimetric detection of Hg2+ ion by green silver nanoparticles synthesized using Dahlia pinnata leaf extract

“…Silver nanoparticles were first prepared through the green route by Gardea-Torresdey et al [16]. After that, many research groups synthesized Ag nanoparticles using leaf extract of Azadirachta indica [17], Hibiscus rosa-sinensis [18], Petroselinum crispum [19], Camellia sinensis [20], Cymbopogon sp. [21], etc.…”